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Transcriptional profiling of chronic clinical hepatic schistosomiasis japonica indicates reduced metabolism and immune responses

Published online by Cambridge University Press:  28 July 2015

GEOFFREY N. GOBERT ,
MELISSA L. BURKE ,
DONALD P. MCMANUS ,
MAGDA K. ELLIS ,
CANDY CHUAH ,
GRANT A. RAMM ,
YUANYUAN WANG  and
YUESHENG LI
GEOFFREY N. GOBERT*
Affiliation:
QIMR Berghofer Medical Research Institute, Brisbane, Qld 4006, Australia
MELISSA L. BURKE
Affiliation:
QIMR Berghofer Medical Research Institute, Brisbane, Qld 4006, Australia
DONALD P. MCMANUS*
Affiliation:
QIMR Berghofer Medical Research Institute, Brisbane, Qld 4006, Australia
MAGDA K. ELLIS
Affiliation:
QIMR Berghofer Medical Research Institute, Brisbane, Qld 4006, Australia
CANDY CHUAH
Affiliation:
QIMR Berghofer Medical Research Institute, Brisbane, Qld 4006, Australia School of Veterinary Sciences, The University of Queensland, Gatton, Qld 4343, Australia School of Medical Sciences, Universiti Sains Malaysia, 16150, Kelantan, Malaysia
GRANT A. RAMM
Affiliation:
QIMR Berghofer Medical Research Institute, Brisbane, Qld 4006, Australia
YUANYUAN WANG
Affiliation:
Hunan Institute of Parasitic Diseases, WHO Collaborating Centre for Research and Control of Schistosomiasis in the Lake Region, China
YUESHENG LI
Affiliation:
QIMR Berghofer Medical Research Institute, Brisbane, Qld 4006, Australia Hunan Institute of Parasitic Diseases, WHO Collaborating Centre for Research and Control of Schistosomiasis in the Lake Region, China
*
*Corresponding authors. QIMR Berghofer Medical Research Institute, Brisbane, Qld 4006, Australia. E-mail: Don.McManus@qimrberghofer.edu.au; Geoffrey.Gobert@qimrberghofer.edu.au
*Corresponding authors. QIMR Berghofer Medical Research Institute, Brisbane, Qld 4006, Australia. E-mail: Don.McManus@qimrberghofer.edu.au; Geoffrey.Gobert@qimrberghofer.edu.au
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Summary

Schistosomiasis is a significant cause of human morbidity and mortality. We performed a genome-wide transcriptional survey of liver biopsies obtained from Chinese patients with chronic schistosomiasis only, or chronic schistosomiasis with a current or past history of viral hepatitis B. Both disease groups were compared with patients with no prior history or indicators of any liver disease. Analysis showed in the main, downregulation in gene expression, particularly those involved in signal transduction via EIF2 signalling and mTOR signalling, as were genes associated with cellular remodelling. Focusing on immune associated pathways, genes were generally downregulated. However, a set of three genes associated with granulocytes, MMP7, CLDN7, CXCL6 were upregulated. Differential gene profiles unique to schistosomiasis included the gene Granulin which was decreased despite being generally considered a marker for liver disease, and IGBP2 which is associated with increased liver size, and was the most upregulated gene in schistosomiasis only patients, all of which presented with hepatomegaly. The unique features of gene expression, in conjunction with previous reports in the murine model of the cellular composition of granulomas, granuloma formation and recovery, provide an increased understanding of the molecular immunopathology and general physiological processes underlying hepatic schistosomiasis.

Keywords

SchistosomiasisSchistosomahepatic fibrosistranscriptomicsclinicalliver
Type
Research Article
Information
Parasitology , Volume 142 , Issue 12 , October 2015 , pp. 1453 - 1468
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Copyright
Copyright © Cambridge University Press 2015 

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