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The truth about in vitro culture of Cryptosporidium species

Published online by Cambridge University Press:  16 November 2017

Panagiotis Karanis
Panagiotis Karanis*
Affiliation:
State Key Laboratory of Plateau Ecology and Agriculture, Centre for Biomedicine and Infectious Diseases, Qinghai Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining, Qinghai, People's Republic of China Medical School, University of Cologne, Cologne, Germany
*
Author for correspondence: Panagiotis Karanis, E-mail: panagiotis.karanis@uk-koeln.de
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Abstract

Cryptosporidium research has focused on the development of infection control, and effective therapy that has thus far been hampered by the inability to culture Cryptosporidium in vitro. Other limitations include inadequate animal models, cumbersome screening procedures for chemotherapeutic approaches and a lack of tools for genetic manipulation. These limitations can, however, be eased by the improvement and focused development of in vitro cultivation. The ability to culture relevant Cryptosporidium isolates in vitro and to propagate the life cycle stages that are responsible for causing disease in an infected host is still a critical link. This ability will facilitate other relevant approaches, e.g., the ability to knockout genes and the application of broader screening for drug discoveries and vaccine developments, in combination with new discoveries on the parasite's basic biology, genetic manipulation and new life cycle stages. Success in this effort represents an essential step towards significant progress in the control of cryptosporidiosis.

Keywords

Cryptosporidiumculturein vitroreview
Type
Review Article
Information
Parasitology , Volume 145 , Issue 7 , June 2018 , pp. 855 - 864
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Copyright
Copyright © Cambridge University Press 2017 

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