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Trypanosoma congolense: the distribution of enzymic variants in East and West Africa

Published online by Cambridge University Press:  06 April 2009

J. K. Gashumba ,
Rose D. Baker  and
D. G. Godfrey
J. K. Gashumba
Affiliation:
Department of Medical Protozoology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St Albans, Herts AL4 0XQ
Rose D. Baker
Affiliation:
Computing Services Section, University of Salford, Salford M5 4WT
D. G. Godfrey
Affiliation:
Department of Medical Protozoology, London School of Hygiene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St Albans, Herts AL4 0XQ
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Summary

A total of 114 stocks of Trypanosoma congolense originating from Kenya, Uganda, Tanzania, Zambia and Sudan, but including, for comparison, stocks from The Gambia, Liberia, Ivory Coast, Nigeria and Cameroun, were compared by isoenzyme electrophoresis for 6 enzymes. The zymodemes were grouped, both from a dendrogram and using a cladistic method, after calculating the dissimilarity, or distance, between profiles. Previous observations are broadly confirmed, the zymodemes clustering separately according to geographical origin and ecological zone. Thus, one group was composed almost entirely of East African stocks, and another of stocks from both East and West Africa, although each group was of savanna origin. A third group was composed of stocks from the humid, rain-forest zones of West Africa, and was particularly characterized by isoenzyme variants of superoxide dismutase and glucose-phosphate isomerase. Two stocks from the Kenyan coast formed a markedly separate group, which may be taxonomically distinct.

Type
Research Article
Information
Parasitology , Volume 96 , Issue 3 , June 1988 , pp. 475 - 486
Copyright
Copyright © Cambridge University Press 1988

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References

REFERENCE

Gashumba, J. K., (1985). Trypanosoma congolense: The distribution of enzymic variants in East Africa. Ph.D. thesis, University of London.Google Scholar
Gashumba, J. K., (1986). Two enzymically distinct stocks of Trypanosoma congolense. Research in Veterinary Science 40, 411–12.Google Scholar
Gashumba, J. K., Gibson, W. C., & Opiyo, E. A., (1986). A preliminary comparison of Trypanosoma simiae and T. congolense by isoenzyme electrophoresis. Acta Tropica 43, 15–19.Google Scholar
Gibson, W. C., (1986). Will the real Trypanosoma b. gambiense please stand up. Parasitology Today 2, 255–7.CrossRefGoogle ScholarPubMed
Gibson, W. C., Dukes, P., & Gashumba, J. K., (1988). Species-specific DNA probes for the identification of African trypanosomes in tsetse flies. Parasitology (in the Press).Google Scholar
Gibson, W. C., Marshall, T. F. de C., & Godfrey, D. G., (1980). Numerical analysis of enzyme polymorphism: a new approach to the epidemiology and taxonomy of the subgenus Trypanozoon. Advances in Parasitology 18, 175–246.CrossRefGoogle Scholar
Gibson, W. C., Mehlitz, D., Lanham, S. M., & Godfbey, D. G., (1978). The identification of Trypanosoma brucei gambiense in Liberian pigs and dogs by isoenzymes and by resistance to human plasma. Tropenmedizin und Parasitologie 29, 335–45.Google Scholar
Godfrey, D. G., (1979). The zymodemes of trypanosomes. Symposia of the British Society for Parasitology 17, 31–53.Google Scholar
Godfrey, D. G., & Kilgour, V., (1976). Enzyme electrophoresis in characterizing the causative organisms of Gambian trypanosomiasis. Transactions of the Royal Society of Tropical Medicine and Hygiene 70, 219–24.Google Scholar
Harris, H., & Hopkinson, D. A., (1976). Handbook of Enzyme Electrophoresis in Human Genetics. Amsterdam: North Holland.Google Scholar
Hoare, C. A., (1972). The Trypanosomes of Mammals. Oxford: Blackwell Scientific Publications.Google Scholar
Jennings, F. W., Whitelaw, D. D., Holmes, P. H., & Urquhart, G. M., (1978). The susceptibility of strains of mice to infection with Trypanosoma congolense. Research in Veterinary Science 25, 399–400.Google Scholar
Lanham, S. M., & Godfrey, D. G., (1970). Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Experimental Parasitology 28, 521–34.CrossRefGoogle ScholarPubMed
Majiwa, P. A. O., Hamers, R., Van Meirvenne, N., & Matthyssens, G., (1986). Evidence for genetic diversity in Trypanosoma (Nannomonas) congolense. Parasitology 93, 291–304.CrossRefGoogle ScholarPubMed
Majiwa, P. A. O., Masake, R. A., Nantulya, V. M., Hamers, R., & Matthyssens, G., (1985). Trypanosoma (Nannomonas) congolense: identification of two karyotypic groups. EMBO Journal 4, 3307–14.CrossRefGoogle ScholarPubMed
Mayr, E., (1953). Concepts of classification and nomenclature in higher organisms and microorganisms. Annals of the New York Academy of Sciences 56, 391–7.Google Scholar
Miles, M. A., (1983). The epidemiology of South American trypanosomiasis—biochemical and immunological approaches and their relevance to control. Transactions of the Royal Society of Tropical Medicine and Hygiene 77, 5–23.CrossRefGoogle ScholarPubMed
Miles, M. A., Povoa, M. M., de Souza, A. A., Lainson, R., Shaw, J. J., & Ketteridge, D. S., (1981). Chagas' disease in the Amazon Basin: II. The distribution of Trypanosoma cruzi zymodemes 1 and 3 in Para State, North Brazil. Transactions of the Royal Society of Tropical Medicine and Hygiene 75, 667–74.Google Scholar
Morrison, W. I., Roelants, G. E., Mayor-Withey, D. S., & Murray, M., (1978). Susceptibility of inbred strains of mice to Trypanosoma congolense: correlation with changes in spleen lymphocyte populations. Clinical and Experimental Immunology 32, 25–40.Google ScholarPubMed
Sneath, P. H. A., & Sokal, R. R., (1973). Numerical Taxonomy. The Principles and Practice of Numerical Taxonomy. San Fransisco: W. H. Freeman and Co.Google Scholar
Stephen, L. E., (1986). Trypanosomiasis. A Veterinary Perspective. Oxford: Pergamon Press.Google Scholar
Ward, J. H., (1963). Hierarchical grouping to optimize an objective function. Journal of the American Statistics Association 58, 236–44.CrossRefGoogle Scholar
World Health Organization (1978). Proposals for the nomenclature of salivarian trypanosomes and for the maintenance of reference collections. Bulletin of the World Health Organization 56, 467–80.Google Scholar
Wraxall, B. G. D., & Culliford, B. J., (1968). A thin-layer starch-gel method for enzyme typing of blood stains. Journal of the Forensic Science Society 8, 81–2.Google Scholar
Young, C. M., & Godfrey, D. G., (1983). Enzyme polymorphism and the distribution of Trypanosoma congolense isolates. Annals of Tropical Medicine and Parasitology 77, 467–81.Google Scholar