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Trypanosoma cruzi nucleoside diphosphate kinase 1 (TcNDPK1) has a broad nuclease activity

Published online by Cambridge University Press:  04 November 2008

M. R. MIRANDA ,
G. E. CANEPA ,
L. A. BOUVIER  and
C. A. PEREIRA
M. R. MIRANDA
Affiliation:
Laboratorio de Biología Molecular de Trypanosoma cruzi (LBMTC), Instituto de Investigaciones Médicas A. Lanari, Universidad de Buenos Aires and CONICET, Combatientes de Malvinas 3150, (1427) Buenos Aires, Argentina
G. E. CANEPA
Affiliation:
Laboratorio de Biología Molecular de Trypanosoma cruzi (LBMTC), Instituto de Investigaciones Médicas A. Lanari, Universidad de Buenos Aires and CONICET, Combatientes de Malvinas 3150, (1427) Buenos Aires, Argentina
L. A. BOUVIER
Affiliation:
Laboratorio de Biología Molecular de Trypanosoma cruzi (LBMTC), Instituto de Investigaciones Médicas A. Lanari, Universidad de Buenos Aires and CONICET, Combatientes de Malvinas 3150, (1427) Buenos Aires, Argentina
C. A. PEREIRA*
Affiliation:
Laboratorio de Biología Molecular de Trypanosoma cruzi (LBMTC), Instituto de Investigaciones Médicas A. Lanari, Universidad de Buenos Aires and CONICET, Combatientes de Malvinas 3150, (1427) Buenos Aires, Argentina
*
*Corresponding author: IDIM, Combatientes de Malvinas 3150, (1427) Bs. As., Argentina. Tel: +5411 4514 8701. Fax: +5411 4523 894. E-mail: cpereira@mail.retina.ar
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Summary

Here, we present the characterization of a trypanosomatid nucleoside diphosphate kinase (TcNDPK1) exhibiting nuclease activity. This is the first identification of a NDPK with this property in trypanosomatid organisms. The recombinant TcNDPK1 protein cleaves not only linear DNA, but also supercoiled plasmid DNA. Additionally, TcNDPK1 is capable of degrading Trypanosoma cruzi genomic DNA. ATP or ADP did not affect the nuclease activity, while the absence of Mg2+ completely inhibits this activity. NDPK and nuclease activities were inhibited at the same temperature, suggesting the presence of related catalytic sites. Furthermore, phenogram analysis showed that TcNDPK1 is close to Drosophila melanogaster and human NDPKs. The unspecific nuclease activity could suggest a participation in cellular processes such as programmed cell death.

Keywords

TrypanosomatidaeTrypanosoma cruziChagas diseasenucleoside diphosphate kinasenuclease
Type
Research Article
Information
Parasitology , Volume 135 , Issue 14 , December 2008 , pp. 1661 - 1666
Copyright
Copyright © 2008 Cambridge University Press

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