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Use of larval, parasitic female and egg antigens from Strongyloides venezuelensis to detect parasite-specific IgG and immune complexes in immunodiagnosis of human strongyloidiasis

Published online by Cambridge University Press:  06 February 2012

A. L. R. GONÇALVES ,
D. S. NUNES ,
M. R. F. GONÇALVES-PIRES ,
M. T. UETA  and
J. M. COSTA-CRUZ
A. L. R. GONÇALVES
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
D. S. NUNES
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
M. R. F. GONÇALVES-PIRES
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
M. T. UETA
Affiliation:
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil
J. M. COSTA-CRUZ*
Affiliation:
Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil
*
*Corresponding author. Laboratório de Diagnóstico de Parasitoses, Instituto de Ciências Biomédicas, Universidade Federal de Uberlândia, Uberlândia, MG, Brazil. Avenida Pará 1720, 38400-902, Uberlândia, MG, Brasil. Tel: +55 34 3218 2187. Fax: +55 34 3118 2333. E-mail: costacruz@ufu.br
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Summary

The aim of this study was to use larval, parasitic female and egg antigens from Strongyloides venezuelensis to detect parasite-specific IgG and immune complexes in human serum samples by enzyme-linked immunosorbent assay (ELISA). In total, 95 serum samples were analysed, consisting of 30 patients harbouring S. stercoralis larvae, 30 healthy subjects and 35 patients with other parasites. Sensitivity, specificity and diagnostic efficiency were calculated. A significant statistical difference was found in the detection of immune complexes and antibodies in patients harbouring S. stercoralis larvae from larval and eggs antigens, with higher positivity using larval antigen. The larval antigen showed the highest values for sensitivity, specificity and diagnostic efficiency in ELISA from detection of immune complexes. For the first time we used IgG anti-larvae, IgG anti-parasitic females or IgG anti-eggs for immune complex detection. We concluded that the association of antibody and immune complex detection could be used in the diagnosis of human strongyloidiasis.

Keywords

antigendiagnosisimmune complexstrongyloidiasis
Type
Research Article
Information
Parasitology , Volume 139 , Issue 7 , June 2012 , pp. 956 - 961
Copyright
Copyright © Cambridge University Press 2012

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References

REFERENCES

Baek, B. K., Islam, M. K. and Matsuda, K. (1998). Viability of eggs, filariform larvae and adults of Strongyloides venezuelensis (Nematoda: Strongyloidea) maintained in vitro. Korean Journal Parasitology 36, 99–107.CrossRefGoogle ScholarPubMed
Baermann, G. (1917). Eine einfache Methode zur Auffindung von Ankylostomun (Nematoden) Larven in Erdproben. Mededeel. mith H Geneesk. Batavia. Lab. Weltevreden Feestbundel 41–47.Google Scholar
Devi, U., Borkakoty, B. and Mahanta, J. (2011). Strongyloidiasis in Assam, India: A community-based study. Tropical Parasitology 1, 30–32.Google ScholarPubMed
Feliciano, N. D., Gonzaga, H. T., Gonçalves-Pires, M. R., Gonçalves, A. L., Rodrigues, R. M., Ueta, M. T. and Costa-Cruz, J. M. (2010). Hydrophobic fractions from Strongyloides venezuelensis for use in the human immunodiagnosis of strongyloidiasis. Diagnostic Microbiology and Infectious Disease 67, 153–161.CrossRefGoogle ScholarPubMed
Ferreira, C. M., Pereira, A. T. M., Souza, R. S., Cassali, G. D., Souza, D. G., Lemos, V. S., Teixeira, M. M. and Negrão-Corrêa, D. (2007). Prevention of changes in airway function facilitates Strongyloides venezuelensis infection in rats. Microbes and Infection 9, 813–820.CrossRefGoogle ScholarPubMed
Genta, R. M. (1989). Global prevalence of strongyloidiasis: critical review with epidemiologic insights into the prevention of disseminated disease. Reviews of Infectious Diseases 11, 755–767.CrossRefGoogle ScholarPubMed
Gonçalves, A. L. R., Rocha, C. A., Gonzaga, H. T., Gonçalves-Pires, M. R., Ueta, M. T. and Costa-Cruz, J. M. (2012). Specific IgG and IgA to larvae, parthenogenetic females and eggs of Strongyloides venezuelensis in the immunodiagnosis of human strongyloidiasis. Diagnostic Microbiology and Infectious Disease 72, 79–84.CrossRefGoogle ScholarPubMed
Gonçalves, A. L. R., Silva, C. V., Ueta, M. T. and Costa-Cruz, J. M. (2010). A new faecal antigen detection system for Strongyloides venezuelensis diagnosis in immunosuppressed rats. Experimental Parasitology 125, 338–341.CrossRefGoogle ScholarPubMed
Gonzaga, H. T., Ribeiro, V. S., Cunha-Júnior, J. P., Ueta, M. T. and Costa-Cruz, J. M. (2011 a). Usefulness of concanavalin-A non-binding fraction of Strongyloides venezuelensis larvae to detect IgG and IgA in human strongyloidiasis. Diagnostic Microbiology and Infectious Disease 70, 78–84.Google Scholar
Gonzaga, H. T., Ribeiro, V. S., Feliciano, N. D., Manhani, M. N., Silva, D. A., Ueta, M. T. and Costa-Cruz, J. M. (2011 b). IgG avidity in differential serodiagnosis of human strongyloidiasis active infection. Immunology Letters 139, 87–92.CrossRefGoogle ScholarPubMed
Gordon, H. M. and Whitlock, H. V. A. (1939). New technique for counting nematode eggs in sheep faeces. Journal of Communication Science in Industrial Organization 12, 17–18.Google Scholar
Koosha, S., Fesharaki, M. and Rokni, M. B. (2004). Evaluation of enzyme-linked immunosorbent assay for the serodiagnosis of strongyloidiasis. Pakistan Journal of Medical Sciences 20, 36–40.Google Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 29, 265–275.CrossRefGoogle Scholar
Lutz, A. (1919). O Schistosomun mansoni e a schistosomose segundo observações feitas no Brasil. Memórias do Instituto Oswaldo Cruz 11, 121–155.CrossRefGoogle Scholar
Machado, E. R., Ueta, M. T., Gonçalves-Pires, M. R. F., Oliveira, J. B. A., Faccioli, L. H. and Costa-Cruz, J. M. (2003). Strongyloides venezuelensis alkaline extract for the diagnosis of human strongyloidiasis by enzyme-linked immunosorbent assay. Memórias do Instituto Oswaldo Cruz 98, 849–851.Google Scholar
Marcos, L. A., Terashima, A., Canales, M. and Gotuzzo, E. (2011). Update on strongyloidiasis in the immunocompromised host. Current Infectious Disease Reports 13, 35–46.CrossRefGoogle ScholarPubMed
Mineo, J. R., Silva, D. A. O., Sopelete, M. C., Leal, G. S., Vidigal, L. H. G., Tápia, L. E. R. and Bacchin, M. I. (2005). Pesquisa na área biomédica: do planejamento á publicação. Uberlândia: EDUFU: Editora da Universidade Federal de Uberlândia, Brazil.CrossRefGoogle Scholar
Paula, F. M. and Costa-Cruz, J. M. (2011). Epidemiological aspects of strongyloidiasis in Brazil. Parasitology 138, 1331–1340.Google Scholar
Rigo, C. R., Lescano, S. Z., Marchi, C. R. and Amato-Neto, V. (2008). Avaliação da resposta imune humoral frente a antígenos de Strongyloides venezuelensis. Revista da Sociedade Brasileira de Medicina Tropical 41, 648–653.CrossRefGoogle ScholarPubMed
Rugai, E., Matton, T. and Brisola, A. P. (1954). Nova técnica para isolar larvas de nematóides das fezes: Modificação do método de Baermann. Revista do Instituto Adolfo Lutz 14, 5–8.CrossRefGoogle ScholarPubMed
Sato, Y. and Toma, H. (1990). Strongyloides venezuelensis infections in mice. International Journal for Parasitology 20, 57–62.Google Scholar
Schaffel, R., Nucci, M., Carvalho, E., Braga, M., Almeida, L., Portugal, R. and Pulcheri, W. (2001). The value of an immunoenzymatic test (enzyme-linked immunosorbent assay) for the diagnosis of strongyloidiasis in patients immunosuppressed by hematologic malignancies. American Journal Tropical Medicine and Hygiene 65, 346–350.CrossRefGoogle ScholarPubMed
Schilter, H. C., Pereira, A. T., Eschnazi, P. D., Fernandes, A., Shim, D., Sousa, A. L., Teixeira, M. and Negrão-Corrêa, D. (2010). Regulation of immune responses to Strongyloides venezuelensis challenge after primary infection with different larvae doses. Parasite Immunology 32, 184–192.CrossRefGoogle ScholarPubMed
Siddiqui, A. A. and Berk, S. (2001). Diagnosis of Strongyloides stercoralis infection. Clinical Infectious Diseases 33, 1040–1047.Google Scholar