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Use of species-specific satellite DNAs as diagnostic probes in the identification of Steinernematidae and Heterorhabditidae entomopathogenic nematodes

Published online by Cambridge University Press:  06 April 2009

E. Grenier ,
E. Bonifassi ,
P. Abad  and
C. Laumond
E. Grenier
Affiliation:
Laboratoire de Biologie des Invertébrés, INRA, BP2078, 06606 Antibes Cedex, France
E. Bonifassi
Affiliation:
Laboratoire de Biologie des Invertébrés, INRA, BP2078, 06606 Antibes Cedex, France
P. Abad
Affiliation:
Laboratoire de Biologie des Invertébrés, INRA, BP2078, 06606 Antibes Cedex, France
C. Laumond*
Affiliation:
Laboratoire de Biologie des Invertébrés, INRA, BP2078, 06606 Antibes Cedex, France
*
*Corresponding author. Tel: + 33 93 67 89 57 Fax: + 33 93 67 89 55. E-mail: laumond@antibes.inra.fr.
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Summary

Three satellite DNAs previously isolated from the entomopathogenic nematodes Steinernema carpocapsae, Heterorhabditis bacteriophora and Heterorhabditis indicus give hybridization signals only with the S. carpocapsae, H. bacteriophora and H. indicus populations tested, indicating that these satellite sequences are species-specific. Because of their reiteration and their variabilities, we have shown that these sequences are able to discriminate at the interspecific level between the Steinernema and Heterorhabditis species, but also at the intraspecific level between S. carpocapsae strains. Furthermore, in simple squashed nematode experiments, we are able to unambiguously identify S. carpocapsae, H. bacteriophora and H. indicus populations. This last procedure is effective even on a single infective juvenile, with the main advantage that it avoids time-consuming DNA extractions.

Keywords

Steinernema carpocapsaeHeterorhabditis bacteriophoraHeterorhabditis indicusspecies-specific probessatellite DNAsintraspecific discrimination
Type
Research Article
Information
Parasitology , Volume 113 , Issue 5 , November 1996 , pp. 483 - 489
Copyright
Copyright © Cambridge University Press 1996

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