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Vasoactive intestinal polypeptide-like and peptide histidine isoleucine-like proteins excreted/secreted by Nippostrongylus brasiliensis, Nematodirus battus and Ascaridia galli

Published online by Cambridge University Press:  06 April 2009

N. Foster  and
D. L. Lee
N. Foster*
Affiliation:
Gastrointestinal Drug Delivery Research Centre, Department of Physiological Sciences, The Medical School, Neivcastle upon Tyne NE2 4HH
D. L. Lee
Affiliation:
Department of Pure and Applied Biology, University of Leeds, Leeds LS2 9JT
*
* Corresponding author. Tel: 0191 2227772. Fax: 0191 2226706. E-mail: N.Foster@ncl.AC.UK.
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Summary

Vasoactive intestinal polypeptide (VIP)-Hke protein was detected by dot blot analysis in the excretions/secretions (E/S) of Nematodirus battus and Ascaridia galli and was confirmed in the E/S of Nippostrongylus brasiliensis. ELISA analysis showed that N. brasiliensis E/S contained the highest proportion of VIP-like protein (28·04 pmoles/mg of total E/S protein) and A. galli E/S contained the lowest (10·89 pmoles/mg of total E/S protein). Peptide histidine isoleucine (PHI)- like protein was detected by dot blot analysis in the E/S products of N. brasiliensis, N. battus and A. galli. ELISA analysis suggested that A. galli E/S contained the highest proportion of PHI (20·77 nmoles/mg of total E/S protein) and N. battus E/S contained the lowest (0·67 nmoles/mg of total E/S protein). The possible significance of VIP-like and PHI-like substances in the E/S of gastrointestinal nematodes is discussed.

Keywords

Nematodirus battusNippostrongylus brasiliensisAscaridia galliexcretions/secretionsvasoactive intestinal polypeptidepeptide histidine isoleucine
Type
Research Article
Information
Parasitology , Volume 113 , Issue 3 , September 1996 , pp. 287 - 292
Copyright
Copyright © Cambridge University Press 1996

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