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Whole genome genetic-typing in yeast using high-density oligonucleotide arrays

Published online by Cambridge University Press:  01 October 1999

E. A. WINZELER ,
B. LEE ,
J. H. McCUSKER  and
R. W. DAVIS
E. A. WINZELER
Affiliation:
Department of Biochemistry, Stanford University School of Medicine, Stanford, CA 94305–5307, USA
J. H. McCUSKER
Affiliation:
Department of Microbiology, 3020, Duke University Medical Center, Durham, NC 27710, USA
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Abstract

Genome sequence information in combination with new technologies has allowed researchers to approach genetic problems in new ways. High-density oligonucleotide arrays were used to probe the genome content of the yeast Saccharomyces cerevisiae. We show that these arrays, containing oligonucleotides complementary to the sequenced strain of S. cerevisiae, can be used to identify open reading frames that are missing or present in higher or lower copy number in related isolates of S. cerevisiae. We apply this method to the characterization of the genome of a strain derived from a clinical isolate of S. cerevisiae. Our results show that the telomeres are the regions with the most variability between the two strains.

Keywords

oligonucleotide arraystelomeresyeasthybridization
Type
Research Article
Information
Parasitology , Volume 118 , Issue 7 , October 1999 , pp. 73 - 80
Copyright
© 1999 Cambridge University Press

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