Hostname: page-component-78c5997874-dh8gc Total loading time: 0 Render date: 2024-11-10T10:08:59.810Z Has data issue: false hasContentIssue false
  • English
  • Français

Distribution of genetic diversity in wild European populations of prickly lettuce (Lactuca serriola): implications for plant genetic resources management

Published online by Cambridge University Press:  25 May 2010

C. C. M. van de Wiel ,
T. Sretenović Rajičić ,
R. van Treuren ,
K. J. Dehmer ,
C. G. van der Linden  and
Th. J. L. van Hintum
C. C. M. van de Wiel*
Affiliation:
Wageningen University and Research Centre Plant Breeding, PO Box 16, 6700 AAWageningen, The Netherlands
T. Sretenović Rajičić
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany
R. van Treuren
Affiliation:
Centre for Genetic Resources, The Netherlands (CGN), Wageningen University and Research Centre, Wageningen, The Netherlands
K. J. Dehmer
Affiliation:
Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany
C. G. van der Linden
Affiliation:
Wageningen University and Research Centre Plant Breeding, PO Box 16, 6700 AAWageningen, The Netherlands
Th. J. L. van Hintum
Affiliation:
Centre for Genetic Resources, The Netherlands (CGN), Wageningen University and Research Centre, Wageningen, The Netherlands
*
*Corresponding author. E-mail: clemens.vandewiel@wur.nl
Get access Rights & Permissions [Opens in a new window]

Abstract

Genetic variation in Lactuca serriola, the closest wild relative of cultivated lettuce, was studied across Europe from the Czech Republic to the United Kingdom, using three molecular marker systems, simple sequence repeat (SSR, microsatellites), AFLP and nucleotide-binding site (NBS) profiling. The ‘functional’ marker system NBS profiling, targeting disease resistance genes of the NBS/LRR family, did not show marked differences in genetic diversity parameters to the other systems. The autogamy of the species resulted in low observed heterozygosity and high population differentiation. Intra-population variation ranged from complete homogeneity to nearly complete heterogeneity. The highest genetic diversity was found in central Europe. The SSR results were compared to SSR variation screened earlier in the lettuce collection of the Centre for Genetic Resources, the Netherlands (CGN). In the UK, practically only a single SSR genotype was found. This genotype together with a few other common SSR genotypes comprised a large part of the plants sampled on the continent. Among the ten most frequent SSR genotypes observed, eight were already present in the CGN collection. Overall, the CGN collection appears to already have a fair representation of genetic variation from NW Europe. The results are discussed in relation to sampling strategies for improving genebank collections of crop wild relatives.

Keywords

collectiongenetic diversityLactucamolecular markersplant genetic resourceswild relatives
Type
Research Article
Information
Plant Genetic Resources , Volume 8 , Issue 2 , August 2010 , pp. 171 - 181
Copyright
Copyright © NIAB 2010

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Boukema, IW, Hazekamp, Th and van Hintum, ThJL (1990) CGN Collection Reviews: The CGN Lettuce Collection. Wageningen: Centre for Genetic Resources, The Netherlands (CGN).Google Scholar
Crute, IR (1992) The role of resistance breeding in the integrated control of downy mildew (Bremia lactucae) in protected lettuce. Euphytica 63: 1–2.CrossRefGoogle Scholar
D'Andrea, L, Broennimann, O, Kozlowski, G, Guisan, A, Morin, X, Keller-Senften, J and Felber, F (2009) Climate change, anthropogenic disturbance and the northward range expansion of Lactuca serriola (Asteraceae). Journal of Biogeography 36: 1573–1587.CrossRefGoogle Scholar
Doyle, JJ and Doyle, JL (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13–15.Google Scholar
Guarino, L, Ramanatha Rao, V and Reid, R (eds) (1995) Collecting Plant Genetic Diversity: Technical Guidelines. Wallingford: CABI.Google Scholar
Hooftman, DAP, Oostermeijer, JGB and Den Nijs, JCM (2006) Invasive behaviour of Lactuca serriola (Asteraceae) in the Netherlands: spatial distribution and ecological amplitude. Basic and Applied Ecology 7: 507–519.CrossRefGoogle Scholar
Kuang, HH, Woo, SS, Meyers, BC, Nevo, E and Michelmore, RW (2004) Multiple genetic processes result in heterogeneous rates of evolution within the major cluster disease resistance genes in lettuce. Plant Cell 16: 2870–2894.CrossRefGoogle ScholarPubMed
Kuang, HH, van Eck, HJ, Sicard, D, Michelmore, R and Nevo, E (2008) Evolution and genetic population structure of prickly lettuce (Lactuca serriola) and its RGC2 resistance gene cluster. Genetics 178: 1547–1558.CrossRefGoogle ScholarPubMed
Lebeda, A, Doležalová, I and Astley, D (2004) Representation of wild Lactuca spp. (Asteraceae, Lactuceae) in world genebank collections. Genetic Resources and Crop Evolution 51: 167–174.CrossRefGoogle Scholar
Lebeda, A, Doležalová, I, Křístková, E, Dehmer, KJ, Astley, D, van de Wiel, CCM and van Treuren, R (2007) Acquisition and ecological characterization of Lactuca serriola L. germplasm collected in the Czech Republic, Germany, the Netherlands and United Kingdom. Genetic Resources and Crop Evolution 54: 555–562.CrossRefGoogle Scholar
Lebeda, A, Kitner, M, Dziechciarková, M, Doležalová, I, Křístková, E and Lindhout, P (2009) An insight into the genetic polymorphism among European populations of Lactuca serriola assessed by AFLP. Biochemical Systematics and Ecology 37: 597–608.CrossRefGoogle Scholar
Mantovani, P, van der Linden, G, Maccaferri, M, Sanguineti, MC and Tuberosa, R (2006) Nucleotide-binding site (NBS) profiling of genetic diversity in durum wheat. Genome 49: 1473–1480.CrossRefGoogle ScholarPubMed
Meudt, HM and Clarke, AC (2007) Almost forgotten or latest practice? AFLP applications, analyses and advances. Trends in Plant Science 12: 106–117.CrossRefGoogle ScholarPubMed
Nei, M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America 70: 3321–3323.CrossRefGoogle ScholarPubMed
Nei, M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89: 583–590.CrossRefGoogle ScholarPubMed
Schlötterer, C (2004) The evolution of molecular markers – just a matter of fashion? Nature Reviews Genetics 5: 63–69.CrossRefGoogle ScholarPubMed
Shen, KA, Chin, DB, Arroyo Garcia, R, Ochoa, OE, Lavelle, DO, Wroblewski, T, Meyers, BC and Michelmore, RW (2002) Dm3 is one member of a large constitutively expressed family of nucleotide binding site-leucine-rich repeat encoding genes. Molecular Plant Microbe Interactions 15: 251–261.CrossRefGoogle ScholarPubMed
Siol, M, Prosperi, J, Bonnin, I and Ronfort, J (2008) How multilocus genotypic pattern helps to understand the history of selfing populations: a case study in Medicago truncatula. Heredity 100: 517–525.CrossRefGoogle ScholarPubMed
Sretenović Rajičić, T, van Hintum, T, Lebeda, A and Dehmer, KJ (2008) Analysis of wild Lactuca accessions: conservation and identification of redundancy. Plant Genetic Resources: Characterization and Utilization 6: 153–163.CrossRefGoogle Scholar
Stahl, EA, Dwyer, G, Mauricio, R, Kreitman, M and Bergelson, J (1999) Dynamics of disease resistance polymorphism at the Rpm1 locus of Arabidopsis. Nature 400: 667–671.CrossRefGoogle ScholarPubMed
Syed, NH, Sorensen, AP, Antonise, R, van de Wiel, CCM, van der Linden, CG, van't Westende, W, Hooftman, DA, den Nijs, HC and Flavell, AJ (2006) A detailed linkage map of lettuce based on SSAP, AFLP and NBS markers. Theoretical and Applied Genetics 112: 517–527.CrossRefGoogle ScholarPubMed
Truco, MJ, Antonise, R, Lavelle, D, Ochoa, O, Kozik, A, Witsenboer, H, Fort, SB, Jeuken, MJW, Kesseli, RV, Lindhout, P, Michelmore, RW and Peleman, J (2007) A high-density, integrated genetic linkage map of lettuce (Lactuca spp.). Theoretical and Applied Genetics 115: 735–746.CrossRefGoogle ScholarPubMed
van de Wiel, C, Arens, P and Vosman, B (1999) Microsatellite retrieval in lettuce (Lactuca sativa L.). Genome 42: 139–149.CrossRefGoogle ScholarPubMed
van der Linden, CG, Wouters, DCAE, Mihalka, V, Kochieva, EZ, Smulders, MJM and Vosman, B (2004) Efficient targeting of plant disease resistance loci using NBS profiling. Theoretical and Applied Genetics 109: 384–393.CrossRefGoogle ScholarPubMed
van Hintum, TJL (2003) Molecular characterisation of a lettuce germplasm collection. In: van Hintum, TJL, Lebeda, A, Pink, D and Schut, J (eds) EUCARPIA Leafy Vegetables 2003 Proceedings of the EUCARPIA Meeting on Leafy Vegetables Genetics and Breeding, Noordwijkerhout, Netherlands, 19–21 March 2003. Wageningen: Centre for Genetic Resources the Netherlands (CGN), pp. 99–104.Google Scholar
van Hintum, TJL (2007) Data resolution: a jackknife procedure for determining the consistency of molecular marker datasets. Theoretical and Applied Genetics 115: 343–349.CrossRefGoogle ScholarPubMed
van Treuren, R and van Hintum, TJL (2009) Comparison of anonymous and targeted molecular markers for the estimation of genetic diversity in ex situ conserved Lactuca. Theoretical and Applied Genetics 119: 1265–1279.CrossRefGoogle ScholarPubMed
van Treuren, R, van Hintum, TJL and van de Wiel, CCM (2008) Marker-assisted optimization of an expert-based strategy for the acquisition of modern lettuce varieties to improve a genebank collection. Genetic Resources and Crop Evolution 55: 319–330.CrossRefGoogle Scholar
Wang, MQ, van den Berg, R, van der Linden, CG and Vosman, B (2008) The utility of NBS profiling for plant systematics: a first study in tuber-bearing Solanum species. Plant Systematics and Evolution 276: 137–148.CrossRefGoogle Scholar
Supplementary material: File

van de Weil supplementary material

van de Weil supplementary material

Download van de Weil supplementary material(File)
File 100.9 KB