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Protein disulphide isomerase promoter sequence analysis of Triticum urartu, Aegilops speltoides and Aegilops tauschii

Published online by Cambridge University Press:  15 March 2011

Arun Prabhu Dhanapal ,
Mario Ciaffi ,
Enrico Porceddu  and
Elisa d'Aloisio
Arun Prabhu Dhanapal
Affiliation:
International Doctoral Programme on Agrobiodiversity, Scuola Superiore Sant'Anna – Plant Genetic Resources, Centro Ricerche ENEA Cassacia, Rome00123, Italy
Mario Ciaffi
Affiliation:
Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Viterbo, Italy
Enrico Porceddu*
Affiliation:
Dipartimento di Agrobiologia e Agrochimica, Università della Tuscia, Viterbo, Italy
Elisa d'Aloisio
Affiliation:
International Doctoral Programme on Agrobiodiversity, Scuola Superiore Sant'Anna – Plant Genetic Resources, Centro Ricerche ENEA Cassacia, Rome00123, Italy
*
*Corresponding author. E-mail: porceddu@unitus.it
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Abstract

Protein disulphide isomerase (PDI) catalyses the formation, reduction and isomerization of disulphide bonds in the newly synthesized secretory proteins. Plant PDIs have been shown to be involved in the folding and deposition of seed storage proteins, which makes this enzyme particularly interesting in wheat, as flour quality is strongly affected by composition and structure of seed storage proteins. In hexaploid wheat cultivar (AABBDD) Chinese Spring (CS), the genomic, complementary DNA and promoter sequences of the three homoeologous gene encoding PDI had been isolated and characterized in a previous study revealing high levels of sequence conservation. In this study, we report the isolation and sequencing of a ~700 bp region, comprising ~600 bp of the putative promoter region and 88 bp of the first exon of the typical PDI gene, in five accessions each from Triticum urartu (AA), Aegilops speltoides (BB) and Aegilops tauschii (DD). Sequence analysis indicated large variation among sequences belonging to the different genomes, while close similarity was found within each species and with the corresponding homoeologous PDI sequences of Triticum aestivum cv. CS (AABBDD) resulting in an overall high conservation of the regulatory motifs conferring endosperm-specific expression.

Keywords

promoterprotein disulphide isomeraseregulatory elementsvariability and wild species
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 338 - 341
Copyright
Copyright © NIAB 2011

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