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TIR approach and stress tolerance indices to identify donor for high-temperature stress tolerance in pepper (Capsicum annuum L.)

Published online by Cambridge University Press:  17 January 2020

Smaranika Mishra Open the ORCID record for Smaranika Mishra [Opens in a new window] ,
R. H. Laxman ,
K. Madhavi Reddy  and
R. Venugopalan
Smaranika Mishra*
Affiliation:
ICAR – Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore560 089, India
R. H. Laxman
Affiliation:
ICAR – Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore560 089, India
K. Madhavi Reddy
Affiliation:
ICAR – Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore560 089, India
R. Venugopalan
Affiliation:
ICAR – Indian Institute of Horticultural Research, Hessaraghatta Lake Post, Bangalore560 089, India
*
*Corresponding author. E-mail: smaranika.mishra@icar.gov.in
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Abstract

Bell pepper or sweet pepper (Capsicum annuum L. var. grossum) is highly susceptible to high-temperature stress (HT). Hence, search for donor across C. annuum for HT tolerance was undertaken by following Temperature Induction Response (TIR) technique. The induction and challenging temperature requirement for TIR screening were standardized in 1 d-old Capsicum seedlings. Forty Capsicum genotypes were evaluated based on the recovery growth (RG) and per cent reduction in recovery growth (%RRG) of the seedlings. The genotypes Punjab Guchhedar and Ajeet 1 were found to have maximum cellular level tolerance (CLT) to HT with higher RG and lower %RRG compared to the non-induced seedlings and HDC 75 was found to have minimum CLT. In order to confirm the findings, another experiment was conducted under managed stress and control conditions. Absolute yield obtained from both the environments were used to calculate stress tolerance indices such as heat susceptibility index, tolerance index, stress tolerance index, mean productivity, geometric mean productivity and yield stability index. Based on these tolerance indices, Punjab Guchhedar and Ajeet 1 were found to be highly tolerant and HDC 75 as highly susceptible. Further, the combined result of TIR and tolerance indices also gave the same result confirming Punjab Guchhedar and Ajeet 1 can be used as a donor for the future breeding programme aimed at evolving high-temperature-tolerant bell pepper cultivars. The result also confirms the fitness of TIR technique to screen Capsicum genotypes for tolerance to HT based on variability in acquired thermotolerance.

Keywords

Capsicum annuumhigh-temperature stresspepperTemperature Induction Responsetolerance indices
Type
Research Article
Information
Plant Genetic Resources , Volume 18 , Issue 1 , February 2020 , pp. 19 - 27
Copyright
Copyright © NIAB 2020

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References

Bouslama, M and Schapaugh, WT (1984) Stress tolerance in soybean Part 1: evaluation of three screening techniques for heat and drought tolerance. Crop Science 24: 933–937.CrossRefGoogle Scholar
Burke, JJ (2001) Identification of genetic diversity and mutations in higher plant acquired thermotolerance. Physiologia Plantarum 112: 167–170.CrossRefGoogle Scholar
Dorostkar, S, Dadkhodaie, A and Heidari, B (2015) Evaluation of grain yield indices in hexaploid wheat genotypes in response to drought stress. Archives of Agronomy and Soil Science 61: 397–413.CrossRefGoogle Scholar
Ehlers, JD and Hall, AE (1998) Heat tolerance of contrasting cowpea lines in short and long days. Field Crop Research 55: 11–21.CrossRefGoogle Scholar
Erickson, AN and Markhart, AH (2001) Flower production, fruit set and physiology of bell pepper during elevated temperature and vapor pressure deficit. Journal of American Society of Horticultural Science 126: 697–702.CrossRefGoogle Scholar
FAO (2017) Faostat. Rome, Italy: Food and Agriculture Organization of the United Nations.Google Scholar
Fernandez, GCJ (1992) Effective selection criteria for assessing plant stress tolerance. Crop Science 28: 13–16.Google Scholar
Fisher, RA and Maurer, R (1978) Drought resistance in wheat cultivars in grain yield response. Journal of Agricultural Research 29: 898–907.Google Scholar
Gangappa, E, Ravi, K and Kumar, GV (2006) Evaluation of groundnut (Arachis hypogaea L.) genotypes for temperature tolerance based on Temperature Induction Response (TIR) technique. Indian Journal of Genetics and Plant Breeding 66: 127–130.Google Scholar
Hassanpanah, D (2010) Evaluation of potato cultivars for resistance against water deficit stress under in vivo conditions. Potato Research 53: 383–392.CrossRefGoogle Scholar
Howarth, CJ, Pollock, CJ and Peacock, JM (1997) Development of laboratory-based methods for assessing seedling thermotolerance in pearl millet. New Phytology 137: 129–139.CrossRefGoogle Scholar
Jamshidi, A and Javanmard, HR (2018) Evaluation of barley (Hordeum vulgare L.) genotypes for salinity tolerance under field conditions using the stress indices. Ain Shams Engineering Journal 9: 2093–2099.CrossRefGoogle Scholar
Jayaprakash, TL, Ramamohan, G, Krishna Prasad, BT, Kumar, G, Prasad, TG and Mathew, MK (1998) Genotypic variability in differential expression of Lea2 and Lea3 genes and proteins in response to salinity stress in Finger millet (Eleusine Coracona Gaertn) and rice (Oryza sativa L.) seedlings. Annals of Botany 82: 513–522.CrossRefGoogle Scholar
Kamrani, M, Hoseini, Y and Ebadollahi, A (2018) Evaluation for heat stress tolerance in durum wheat genotypes using stress tolerance indices. Archives of Agronomy and Soil Science 64: 38–45.CrossRefGoogle Scholar
Keeler, SJ, Boettger, CM, Haynes, JG, Kuches, KA, Johnson, MM, Thureen, DL, Keeler, CL and Kitto, SL (2000) Acquired thermotolerance and expression of the HSP100/ClpB genes of lima bean. Plant Physiology 123: 1121–1132.CrossRefGoogle Scholar
Kheir, EA, Sheshshayee, MS, Prasad, TG and Udayakumar, M (2012) Temperature induction response as a screening technique for selecting high temperature-tolerant cotton lines.Journal of Cotton Science 16: 190–199.Google Scholar
Khodarahmpour, Z and Choukan, R (2011) Study of the genetic variation of maize (Zea mays L.) inbred lines in heat stress condition using cluster analysis. Seed and Plant improvement Journal 4: 1–27.Google Scholar
Krishnan, M, Nguyen, HT and Burke, JJ (1989) Heat-shock protein synthesis and thermal tolerance in wheat. Plant Physiology 90: 140–145.CrossRefGoogle Scholar
Kumar, G, Krishnaprasad, BT, Savitha, M, Gopalakrishna, R, Mukhopadhyay, K and Ramamohan, G (1999) Enhanced expression of heat-shock proteins in thermo-tolerant lines of sunflower and their progenies selected on the basis of temperature-induction response. Theoritical and Applied Genetics 99: 359–367.CrossRefGoogle Scholar
Lokesh, B, Srikanthbabu, V, Prasad, TG, Badigannavar, A and Udayakumar, M (2004) Membrane thermo stability as a screening tool to identify high temperature stress tolerant groundnut (Arachis hypogaea L.) lines using temperature induction response approach. Journal of Plant Biology 31: 53–59.Google Scholar
Raghavendra, T, Jayalakshmi, V and Venkatesh Babu, D (2017) Temperature Induction Response (TIR) – a novel physiological approach for thermotolerant genotypes in chickpea (Cicer arietinum L.). Indian Journal of Agricultural Research 51: 252.CrossRefGoogle Scholar
Rosielle, AA and Hamblin, J (1981) Theoretical aspects of selection for yield in stress and non-stress environments. Crop Science 21: 943–946.CrossRefGoogle Scholar
SAS Institute Inc. (2012) Statistical Analysis System, V 9.3. Carry, NC: SAS Institute Inc.Google Scholar
Senthil-Kumar, M and Udayakumar, M (2004) Development of thermotolerant tomato (Lycopersicon esculentum Mill.) lines: an approach based on mutagenesis. Journal of Plant Biology 31: 139–148.Google Scholar
Senthil-Kumar, M, Kumar, G, Srikanthbabu, V and Udayakumar, M (2006) Assessment of variability in acquired thermotolerance: potential option to study genotypic response and the relevance of stress genes. Journal of Plant Physiology 164: 111–125.CrossRefGoogle Scholar
Srikanthbabu, V, Kumar, G, Krishnaprasad, BT, Gopalakrishna, R, Savitha, M and Udayakumar, M (2002) Identification of pea genotypes with enhanced thermotolerance using temperature induction response technique (TIR). Journal of Plant Physiology 159: 535–545.CrossRefGoogle Scholar
Uma, S, Prasad, TG and Udayakumar, M (1995) Genetic variability in recovery growth and synthesis of stress proteins in response to polyethylene glycol and salt stress in Finger millet. Annals of Botany 76: 43–49.CrossRefGoogle Scholar
Venkatesh Babu, D, Sudhakar, P and Sharath Kumar Reddy, Y (2013) Screening of thermotolerant ragi genotypes at seedling stage using TIR technique. The Bioscan 8: 1493–1495.Google Scholar
Vidya, SM, Laxman, RH, Bhatt, RM, Rekha, A, Sunoj John, VS and Ravishankar, KV (2017) Temperature induction response technique: a screening tool for evaluation of banana cultivars for thermotolerance. Indian Journal of Plant Physiology 22: 79–84.CrossRefGoogle Scholar
Yan, J, Yu, L and Xuan, J (2016) De novo transcriptome sequencing and gene expression profiling of spinach (Spinacia oleracea L.) leaves under heat stress. Scientific Reports 6: 19473.CrossRefGoogle Scholar
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