Hostname: page-component-78c5997874-lj6df Total loading time: 0 Render date: 2024-11-13T11:15:48.671Z Has data issue: false hasContentIssue false
  • English
  • Français

Analysis of genetic diversity in Citrus

Published online by Cambridge University Press:  15 June 2011

François Luro ,
Julia Gatto ,
Gilles Costantino  and
Olivier Pailly
François Luro*
Affiliation:
Unité de Recherche 1103 Génétique et Ecophysiologie de la Qualité des Agrumes (GEQA) station de recherche INRA, 20230 San Giuliano, Corse, France
Julia Gatto
Affiliation:
Unité de Recherche 1103 Génétique et Ecophysiologie de la Qualité des Agrumes (GEQA) station de recherche INRA, 20230 San Giuliano, Corse, France
Gilles Costantino
Affiliation:
Unité de Recherche 1103 Génétique et Ecophysiologie de la Qualité des Agrumes (GEQA) station de recherche INRA, 20230 San Giuliano, Corse, France
Olivier Pailly
Affiliation:
Unité de Recherche 1103 Génétique et Ecophysiologie de la Qualité des Agrumes (GEQA) station de recherche INRA, 20230 San Giuliano, Corse, France
*
*Corresponding author. E-mail: luro@corse.inra.fr
Get access Rights & Permissions [Opens in a new window]

Abstract

Sugar and acidity levels are the main criteria of general fruit quality and for citrus juices pulp, in particular. The constituents of the acidity (organic acids) and the sweetness (glucose, fructose and sucrose) and the genes involved in their regulation have seldom been used to explore Citrus genetic diversity. We evaluated the juice composition of primary metabolic components for 87 varieties belonging to the eight major Citrus species grown under the same environmental and cultivation conditions by HPLC. We investigated the sequence polymorphism of nine candidate genes encoding for key enzymes of sugars and organic acids metabolic pathways by single strand conformation polymorphism (SSCP). Whatever the biochemical or molecular analyses, the observed structure of Citrus diversity was organized around three groups corresponding to the ancestral species (mandarin, pummelo and citron). As expected, the secondary species were closely related to their putative ancestors except for Citrus aurantium. Biochemical diversity was strongly correlated to molecular SSCP diversity at the genus level but not at the intraspecific level. Compared with other molecular marker types, higher diversity has been observed with SSCP technology, which makes it suitable for future quantitative trait loci mapping approach on gene polymorphism in citrus pulp acidity and sweetness regulation.

Keywords

acidityCitrus sppgenetic diversitysingle strand conformation polymorphismsweetness
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 218 - 221
Copyright
Copyright © NIAB 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Albertini, MV, Carcouet, E, Pailly, O, Gambotti, C, Luro, F and Berti, L (2006) Changes in organic acids and sugars during early stages of development of acidic and acidless citrus fruit. Journal of Agricultural and Food Chemistry 54: 8335–8339.CrossRefGoogle ScholarPubMed
Bain, JM (1958) Morphological, anatomical and physiological changes in the developing fruit of the Valencia orange Citrus sinensis (L.) Osbeck. Ausralian Journal of Botany 6: 1–24.CrossRefGoogle Scholar
Barkley, NA, Roose, ML, Krueger, RR and Federici, CT (2006) Assessing genetic diversity and population structure in a Citrus germplasm collection utilizing simple sequence repeat markers (SSRs). Theoritical and Applied Genetics 112: 1519–1531.CrossRefGoogle Scholar
Barret, HC and Rhodes, AM (1976) A numerical taxonomic study of affinity relationships in cultivated Citrus and its close relatives. Systematic Botany 1: 105–136.CrossRefGoogle Scholar
Bogin, E and Wallace, A (1966) Organic acid synthesis and accumulation in sweet and sour lemon fruits. Journal of American Society of Horticultural Science 89: 182–194.Google Scholar
Canel, C, Bailey-Serres, JN and Roose, ML (1995) In vitro [14C] citrate uptake by tonoplast vesicles of acidless Citrus juice cells. Journal of American Society of Horticultural Science 120: 510–514.CrossRefGoogle Scholar
Cercos, M, Soler, G, Iglesias, DJ, Gadea, J, Forment, J and Talon, M (2006) Global analysis of gene expression during development and ripening of citrus fruit flesh: a proposed mechanism for citric acid utilization. Plant Molecular Biology 62: 513–527.CrossRefGoogle ScholarPubMed
Dice, LR (1945) Measures of the amount of ecologic association between species. Ecology 26: 297–302.CrossRefGoogle Scholar
Doyle, JJ and Doyle, JL (1987) Isolation of DNA from fresh plant tissue. Focus 12: 13–15.Google Scholar
Luro, F, Rist, D and Ollitrault, P (2001) Evaluation of genetic relationships in Citrus genus by means of sequence tagged microsatellites. Acta Horticultarae 546: 537–542.Google Scholar
Luro, F, Costantino, G, Argout, X, Froelicher, Y, Terol, J, Talon, M, Wincker, P, Ollitrault, P and Morillon, R (2008) Transferability of the EST-SSRs developed on Nules clementine (Citrus clementina Hort ex Tan) to other Citrus species and their effectiveness for genetic mapping. BMC Genomics 9: 287.CrossRefGoogle Scholar
Markoff, A, Savov, A, Vladimirov, V, Bogdanova, N, Kremensky, I and Ganev, V (1997) Optimization of single-strand conformation polymorphism analysis in the presence of polyethylene glycol. Clinical Chemistry 43: 30–33.CrossRefGoogle ScholarPubMed
Marsh, KB, Richardson, AC and Erner, Y (2003) Effect of environmental conditions and horticultural practices on citric acid content In: International Society of Citriculture (ed.). Proceedings of the 9th International Citriculture Congress, Orlando, pp. 640–643.Google Scholar
Nicolosi, E, Deng, ZN, Gentile, A, La Malfa, S, Continella, G and Tribulato, E (2000) Citrus phylogeny and genetic origin of important species as investigated by molecular markers. Theoritical and Applied Genetics 100: 1155–1166.CrossRefGoogle Scholar
Perrier, X, Flori, A and Bonnot, F (2003) Data analysis methods. In: Hamon, P, Seguin, M, Perrier, X and Glaszmann, JC (eds) Genetic Diversity of Cultivated Tropical Plants. Montpellier: Enfield Science Publishers, pp. 43–76.Google Scholar
Sinclair, WB (1984) Organic acids of lemon fruits. In: The Regents of the University of California (ed.) The Biochemistry and Physiology of the Lemon and Other Citrus Fruits. Oakland, CA: University of California, pp. 109–156.Google Scholar
Talon, M and Gmitter, F Jr (2008) Citrus genomics. International Journal of Plant Genomics. 2008: 528361.CrossRefGoogle ScholarPubMed
Ting, SV and Attaway, JA (1971) Citrus fruits. In: Hulme, AC (ed.) The Biochemistry of Fruits and their Products. London: Academic Press, pp. 107–169.Google Scholar
Tucker, GA (1993) Introduction. In: Seymour, G, Taylor, J and Tucker, G (eds) Biochemistry of Fruit Ripening. London: Chapman and Hall, pp. 1–37.Google Scholar