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Analysis of the sequence of ITS1 and ITS2 regions of Three Mentha species

Published online by Cambridge University Press:  14 September 2016

Elena Apostolova ,
Krasimir Todorov ,
Ivanka Dimitrova-Dyulgerova ,
Plamen Stoyanov ,
Rumen Mladenov ,
Galina Yahubyan  and
Samir Naimov
Elena Apostolova*
Affiliation:
Department of Plant Physiology and Molecular Biology, University of Plovdiv Paisii Hilendarski, Plovdiv, Bulgaria
Krasimir Todorov
Affiliation:
Department of Botany and Teaching Methods in Biology, University of Plovdiv Paisii Hilendarski, Plovdiv, Bulgaria
Ivanka Dimitrova-Dyulgerova
Affiliation:
Department of Botany and Teaching Methods in Biology, University of Plovdiv Paisii Hilendarski, Plovdiv, Bulgaria
Plamen Stoyanov
Affiliation:
Department of Botany and Teaching Methods in Biology, University of Plovdiv Paisii Hilendarski, Plovdiv, Bulgaria
Rumen Mladenov
Affiliation:
Department of Botany and Teaching Methods in Biology, University of Plovdiv Paisii Hilendarski, Plovdiv, Bulgaria
Galina Yahubyan
Affiliation:
Department of Plant Physiology and Molecular Biology, University of Plovdiv Paisii Hilendarski, Plovdiv, Bulgaria
Samir Naimov
Affiliation:
Department of Plant Physiology and Molecular Biology, University of Plovdiv Paisii Hilendarski, Plovdiv, Bulgaria
*
*Corresponding author. E-mail: elena.apost@gmail.com
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Abstract

Mentha (Nepetoideae, Lamiaceae) is a taxonomically complex genus consisting of 29 species and 11 different hybrids including a number of economically important species among them medical plants used for more than 250 years. Because of its high incidence of polyploidy, diverse morphology, high degree of chromosome number variations and interspecies hybridization, Mentha is considered to be a taxonomical challenge. In this paper, we describe cloning and sequencing of internal transcribed spacers (ITS) 1 and 2 of three mint species: Mentha aquatic L., Mentha longifolia L. (Huds) and Mentha piperita L., collected from natural habitats of Bulgaria. The applicability of ITS1 and ITS2 for molecular fingerprinting and further comparative studies, including the newly obtained ITS1 and ITS2 sequences, was tested in phylogenetic analysis of all eight Mentha species, found in Bulgarian flora.

Keywords

ITS1ITS2M. aquaticaM. longifoliaM. piperita
Type
Short Communications
Information
Plant Genetic Resources , Volume 15 , Issue 6 , December 2017 , pp. 563 - 565
Copyright
Copyright © NIAB 2016 

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