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Assessing genetic diversity, population structure and gene flow in the Korean red bean [Vigna angularis (Willd.) Ohwi & Ohashi] using SSR markers

Published online by Cambridge University Press:  06 February 2012

Kim Banni ,
Kyaw Thu Moe  and
Yong-Jin Park
Kim Banni
Affiliation:
Department of Plant Resources, College of Industrial Sciences, Kongju National University, Yesan340-702, Republic of Korea
Kyaw Thu Moe
Affiliation:
Department of Plant Resources, College of Industrial Sciences, Kongju National University, Yesan340-702, Republic of Korea
Yong-Jin Park*
Affiliation:
Department of Plant Resources, College of Industrial Sciences, Kongju National University, Yesan340-702, Republic of Korea Legume Bio-Resource Center of Green Manure (LBRCGM), Kongju National University, Yesan340-702, Republic of Korea
*
*Corresponding author. E-mail: yjpark@kongju.ac.kr
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Abstract

Red bean, also known as azuki bean [Vigna angularis (Willd.) Ohwi & Ohashi], belongs to a group of legumes (family Fabaceae). The name azuki is a transliteration of the native Japanese name from the Chinese word Shōzu, which means small bean. In Korea, it is known as pat. In total, 178 red bean accessions were taken to analyse the genetic diversity, population structure and gene flow using 39 polymorphic simple sequence repeat markers. A total of 431 alleles were detected, with an average of 11 alleles per locus, among the 178 tested red bean accessions. Forty-six specific alleles were identified with 20 loci. Locus CEDG090 had the highest number (n = 22) of alleles, whereas only two alleles were observed at loci CEDG144 and CEDC018. The proportion of different alleles for microsatellite loci was analysed using a microsatellite toolkit. In locus CEDG029, one allele was shared in all the three groups of varieties and species, and three alleles were shared between the wild ancestors and cultivated varieties, while in locus CEDG090, one allele was shared in all the three groups and 12 alleles were shared between the wild ancestors and cultivated varieties. Our findings describe the genetic relationships and population structure of the red bean in Korea and will be useful for designing effective breeding programmes and broadening the genetic base of commercial varieties. Moreover, the results demonstrate substantial gene flow from the red bean to nearby wild relatives in a given region.

Keywords

gene flowgenetic diversityintrogressionpopulation structurered beanSSR markers
Type
Research Article
Information
Plant Genetic Resources , Volume 10 , Issue 1 , April 2012 , pp. 74 - 82
Copyright
Copyright © NIAB 2012

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Supplementary material: File

Park Supplementary Table

Supplementary Online material Table S1. Information on Korean red bean (178) accessions used in this study and their inferred clusters by model based analysis.

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