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Assessing the leaf shape dynamic through marker–trait association under drought stress in a rice germplasm panel

Published online by Cambridge University Press:  07 January 2022

Mayuri D. Mahalle Open the ORCID record for Mayuri D. Mahalle [Opens in a new window] ,
S. K. Chetia ,
P. C. Dey ,
R. N. Sarma ,
A. R. Baruah ,
R. C. Kaldate ,
Rahul K. Verma  and
M. K. Modi
Mayuri D. Mahalle
Affiliation:
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam 785013, India
S. K. Chetia
Affiliation:
Regional Agricultural Research Station, Assam Agricultural University, Titabor, Assam 785630, India
P. C. Dey
Affiliation:
Regional Agricultural Research Station, Assam Agricultural University, Titabor, Assam 785630, India
R. N. Sarma
Affiliation:
Department of Plant Breeding and Genetics, Assam Agricultural University, Jorhat 785013, India
A. R. Baruah
Affiliation:
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam 785013, India
R. C. Kaldate
Affiliation:
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam 785013, India
Rahul K. Verma
Affiliation:
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam 785013, India
M. K. Modi*
Affiliation:
Department of Agricultural Biotechnology, Assam Agricultural University, Jorhat, Assam 785013, India
*
Author for correspondence: M. K. Modi, E-mail: mkmodi@gmail.com
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Abstract

The flag leaf acts as a functional leaf in rice, Oryza sativa L., primarily supplying photosynthate to the developing grains and influencing yields to a certain extent. Drought stress damages the leaf physiology, severely affecting grain fertility. Autumn rice of northeast India is called locally as ‘ahu’ rice, and is known for its drought tolerance. Exploring diverse germplasm resources at the morphological level using an association mapping approach can aid in identifying the genomic regions influencing leaf shape dynamics. A marker–trait association (MTA) study was carried out using 95 polymorphic SSR markers and a panel of 273 ahu rice germplasm accessions in drought stress and irrigated conditions. The trials suggest that at the vegetative stage, drought stress significantly affects leaf morphology. The leaf physiology of some tolerant accessions was relatively little affected by stress and these can be considered as ideal varieties for drought conditions. The phenotypic coefficient of variance and genotypic coefficient of variance values implied moderate to high variability for the leaf traits studied. Analysis of molecular variance inferred that 11% of variation in the germplasm panel was due to differences between populations, while the remaining 89% may be attributed to a difference within subgroups formed through STRUCTURE analysis. Using the mixed linear model approach revealed 11 MTAs explaining between 4.5 and 20.0% of phenotypic variance at P > 0.001 for all the leaf traits. The study concludes that ahu rice germplasm is extremely diverse and can serve as a valuable resource for mining desirable alleles for drought tolerance.

Keywords

Ahu riceflag leafgrain yieldlandracesmarker–trait associationOryza sativa L
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 6 , December 2021 , pp. 477 - 483
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

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