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Assessment of genetic variability amongst cultivated populations of Khasi mandarin (Citrus reticulata Blanco) detected by ISSR

Published online by Cambridge University Press:  08 April 2021

Karishma Kashyap ,
Rasika M. Bhagwat  and
Sofia Banu Open the ORCID record for Sofia Banu [Opens in a new window]
Karishma Kashyap
Affiliation:
Department of Bioengineering and Technology, GUIST, Gauhati University, Guwahati781014, India
Rasika M. Bhagwat
Affiliation:
Teri, Western Regional Centre, Mumbai400614, India
Sofia Banu*
Affiliation:
Department of Bioengineering and Technology, GUIST, Gauhati University, Guwahati781014, India
*
*Corresponding author. E-mail: sofiabanu2@gmail.com
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Abstract

Khasi mandarin (Citrus reticulata Blanco) is a commercial mandarin variety grown in northeast India and one of the 175 Indian food items included in the global first food atlas. The cultivated plantations of Khasi mandarin grown prominently in the lower Brahmaputra valley of Assam, northeast India, have been genetically eroded. The lack in the efforts for conservation of genetic variability in this mandarin variety prompted diversity analysis of Khasi mandarin germplasm across the region. Thus, the study aimed to investigate genetic diversity and partitioning of the genetic variations within and among 92 populations of Khasi mandarin collected from 10 cultivated sites in Kamrup and Kamrup (M) districts of Assam, India, using Inter-Simple Sequence Repeat (ISSR) markers. The amplification of genomic DNA with 17 ISSR primers yielded 216 scorable DNA amplicons of which 177 (81.94%) were polymorphic. The average polymorphism information content was 0.39 per primer. The total genetic diversity (HT = 0.28 ± 0.03) was close to the diversity within the population (HS = 0.20 ± 0.01). A high mean coefficient of gene differentiation (GST = 0.29) reflected a high level of gene flow (Nm = 1.22), indicating high genetic differentiation among the populations. Analysis of Molecular Variance (AMOVA) showed 78% of intra-population differentiation, 21% among the population and 1% among the districts. The obtained results indicate the existence of a high level of genetic diversity in the cultivated Khasi mandarin populations, indicating the need for preservation of each existing population to revive the dying out orchards in northeast India.

Keywords

AMOVAgene flowgenetic diversityKhasi mandarinpolymorphism
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 2 , April 2021 , pp. 122 - 132
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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