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A baseline study of vicine–convicine levels in faba bean (Vicia faba L.) germplasm

Published online by Cambridge University Press:  30 April 2013

Khalil Khamassi ,
Fayçal Ben Jeddi ,
Doug Hobbs ,
Jose Irigoyen ,
Fred Stoddard ,
Donal M. O'Sullivan  and
Huw Jones
Khalil Khamassi
Affiliation:
National Institute of Agricultural Botany, Huntingdon Road, CambridgeCB3 0LE, UK Institut National Agronomique de Tunisie (INAT), Unité de Recherche Cultures Maraîchères et Florale, 43 Avenue Charles Nicolle 1082 cité Mahrajène, Tunis, Tunisia
Fayçal Ben Jeddi
Affiliation:
Institut National Agronomique de Tunisie (INAT), Unité de Recherche Cultures Maraîchères et Florale, 43 Avenue Charles Nicolle 1082 cité Mahrajène, Tunis, Tunisia
Doug Hobbs
Affiliation:
National Institute of Agricultural Botany, Huntingdon Road, CambridgeCB3 0LE, UK
Jose Irigoyen
Affiliation:
National Institute of Agricultural Botany, Huntingdon Road, CambridgeCB3 0LE, UK
Fred Stoddard
Affiliation:
University of Helsinki, Helsinki, Finland
Donal M. O'Sullivan
Affiliation:
National Institute of Agricultural Botany, Huntingdon Road, CambridgeCB3 0LE, UK Department of Agriculture, Policy and Development, University of Reading, Reading, UK
Huw Jones*
Affiliation:
National Institute of Agricultural Botany, Huntingdon Road, CambridgeCB3 0LE, UK
*
*Corresponding author. E-mail: huw.jones@niab.com
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Abstract

Vicine and convicine are anti-nutritional compounds that accumulate in the cotyledons of faba beans. When humans consume beans with high levels of these compounds, it can cause a condition called favism in individuals harbouring a deficiency in the activity of their glucose-6-phosphate dehydrogenase. When faba beans are used in animal feeds, there can be effects on performance. These concerns have resulted in increasing interest within plant breeding in developing low vicine and convicine faba bean germplasm. In order to facilitate this objective, we developed a rapid and robust screening method for vicine and convicine, capable of distinguishing between faba beans that are either high (wild type) or low in vicine and convicine. In the absence of reliable commercial reference materials, we report an adaptation of a previously published method where a biochemical assay and spectral data were used to confirm the identity of our analytes, vicine and convicine. This method could be readily adopted in other facilities and open the way to the efficient exploitation of diverse germplasm in regions where faba beans play a significant role in human nutrition. We screened a collection of germplasm of interest to a collaborative plant breeding programme developing between the National Institute for Agricultural Botany in the UK and L'Institut Nationale d'Agronomie de Tunisie in Tunisia. We report the results obtained and discuss the prospects for developing molecular markers for the low vicine and convicine trait.

Keywords

faba beanHPLCViciavicine–convicine
Type
Research Article
Information
Plant Genetic Resources , Volume 11 , Issue 3 , December 2013 , pp. 250 - 257
Copyright
Copyright © NIAB 2013 

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