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Breeding medicinal plant, periwinkle [Catharanthus roseus (L) G. Don]: a review

Part of: Evolving Trends in Plant Based Drug Discovery

Published online by Cambridge University Press:  02 May 2016

R. N. Kulkarni ,
K. Baskaran  and
Tripta Jhang
R. N. Kulkarni*
Affiliation:
CSIR – Central Institute of Medicinal Plants (CIMAP) Research Centre, Bangalore 560 065, Karnataka, India
K. Baskaran
Affiliation:
CSIR – Central Institute of Medicinal Plants (CIMAP) Research Centre, Bangalore 560 065, Karnataka, India
Tripta Jhang
Affiliation:
Genetics & Plant Breeding Division, CSIR–CIMAP, Lucknow 226 015, UP, India
*
*Corresponding author. E-mail: krnpbg@yahoo.co.in
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Abstract

Periwinkle [Catharanthus roseus (L) G. Don] has become one of the very extensively investigated medicinal plants after the discovery of two powerful anti-cancer alkaloids, vinblastine and vincristine, in its leaves more than 50 years ago. These alkaloidal drugs are still in clinical use. Also, periwinkle is still the only source of these alkaloids and their precursors, catharanthine and vindoline. Low concentrations of these alkaloids in the plant and, therefore, high costs of their extraction have led to tremendous efforts towards understanding their biosynthesis and exploration of alternate ways of their production such as, chemical synthesis, cell, tissue and hairy root cultures, and metabolic engineering of heterologous organisms. Literature on this plant is quite voluminous, with an average of about 80 publications per year during last three decades (1985–2015). Nearly 60% of these publications are on physiology, biochemistry, cell and tissue culture, phytochemistry, metabolic and genetic engineering aspects. In spite of these efforts, an economically viable alternative to field-grown periwinkle plants as a source of these alkaloids has not yet been found. Biosynthesis of C. roseus alkaloids is a complex process involving many genes, enzymes, regulators, inter- and intra-cellular transporters, cell types, organelles and tissues and its current understanding is still considered to be incomplete to produce C. roseus alkaloids through metabolic engineering/synthetic biology. Till such time, breeding periwinkle varieties with higher concentrations of anti-cancer alkaloids for cultivation can be an alternate approach to meet the demand for these alkaloids and reduce their costs. While literature on cell and tissue culture, phytochemistry, metabolic and genetic engineering aspects of periwinkle has been reviewed periodically, crop production and plant breeding aspects have received little attention. In this paper, an attempt has been made to bring together published information on genetics and breeding of periwinkle as a medicinal plant. Some probable constraints which may have hindered taking up periwinkle breeding are identified. Initially, quite a few attempts have been made at genetic improvement of periwinkle through induced polyploidy, and subsequently through induced mutagenesis. Mutations, both natural and induced, provide a valuable resource for use in breeding and in functional and reverse genomics research. It is only during last 6–7 years, genetic diversity has been assessed using molecular markers and very recently molecular markers have been identified for marker-assisted selection for alkaloid yield.

Keywords

anti-cancer alkaloidsautotetraploidsbreeding behaviourcleistogamycytologyinduced mutantsinheritancemarker-assisted selectionsecondary metabolism
Type
Research Article
Information
Plant Genetic Resources , Volume 14 , Special Issue 4: Evolving trends in plant based drug discovery , December 2016 , pp. 283 - 302
Copyright
Copyright © NIAB 2016 

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References

Arens, H, Stockigt, J, Weiler, EW and Zenk, MH (1978) Radioimmunoassays for the determination of the indole alkaloids ajmalicine and serpentine in plants. Planta Medica 34: 37–46.CrossRefGoogle Scholar
Baskaran, K, Srinivas, KVNS and Kulkarni, RN (2013) Two induced macro-mutants of periwinkle with enhanced contents of leaf and root alkaloids and their inheritance. Industrial Crops and Products 43: 701–703.CrossRefGoogle Scholar
Belhaj, K, Chaparro-Garcia, A, Kamoun, S and Nekrasov, V (2013) Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods 9: 39.CrossRefGoogle ScholarPubMed
Boke, NH (1948) Development of perianth in Vinca rosea L. American Journal of Botany 35: 413–423.Google Scholar
Boke, NH (1949) Development of the stamens and carpels in Vinca rosea L. American Journal of Botany 36: 535–547.CrossRefGoogle Scholar
Bowman, RN (2000) Method of producing hybrid Catharanthus using male sterility. US Patent 6,166,306 A, 26 December 2000.Google Scholar
Brown, S, Clastre, M, Courdavault, V and O'Connor, SE (2015) De novo production of the plant-derived alkaloid strictosidine in yeast. Proceedings of the National Academy of Sciences of the United States of America 112: 3205–3210. doi: 10.1073/pnas.1423555112.CrossRefGoogle ScholarPubMed
Brumlop, S and Finckh, MR (2011) Applications and Potentials of Marker Assisted Selection (MAS) in Plant Breeding. Bonn, Germany: Federal Agency for Nature Conservation.Google Scholar
Bruneton, J (1995) Pharmacognosy, Phytochemistry, Medicinal Plants. Paris: Lavoisier.Google Scholar
Campos-Tamayo, F, Hernandez-Dominguez, E, and Vazquez-Flota, F (2008) Vindoline formation in shoot cultures of Catharanthus roseus is synchronously activated with morphogenesis through the last biosynthetic step. Annals of Botany 102: 409–415.CrossRefGoogle ScholarPubMed
Chaudhary, S, Sharma, V, Prasad, M, Bhatia, S, Tripathi, BN, Yadava, G and Kumar, S (2011) Characterization and genetic linkage mapping of the horticulturally important mutation leafless inflorescence (lli) in periwinkle Catharanthus roseus . Scientia Horticulturae 129: 142–153.Google Scholar
Chaudhary, S, Pandey, R, Sharma, V, Tripathi, BN and Kumar, S (2013) Detection and mapping of QTLS affecting contents of pharmaceutical alkaloids in leaf and root of Catharanthus roseus . Agricultural Research 2: 9–23.CrossRefGoogle Scholar
Comai, L, Young, K, Till, BJ, Reynolds, SH, Greene, EA, Codomo, CA, Enns, LC, Johnson, JE, Burtner, C, Odden, AR and Henikoff, S (2004) Efficient discovery of nucleotide polymorphisms in populations by ECOTILLING. The Plant Journal 37: 778–786.CrossRefGoogle ScholarPubMed
Cragg, GM and Newman, DJ (2005) Plants as a source of anti-cancer agents. Journal of Ethnopharmacology 100: 72–79.CrossRefGoogle ScholarPubMed
Dehghan, E, Häkkinen, ST, Oksman-Caldentey, K-M and Ahmadi, FS (2012) Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.). Plant Cell Tissue and Organ Culture 110: 35–44.Google Scholar
De Padua, DS, Barrion, AA, Casal, CMV and De La Cruz, MaPR (1992) Karyomorphology of chichirica Catharanthus roseus (L.) Don. Philippine Journal of Science 121: 299–303.Google Scholar
Dhawan, OP and Lavania, UC (1996) Enhancing the productivity of secondary metabolites via induced polyploidy: a review. Euphytica 87: 81–89.Google Scholar
Dias, DA, Urban, S and Roessner, U (2012) A historical overview of natural products in drug discovery. Metabolites 2: 303–336.Google Scholar
Di Fiore, S, Li, Q, Leech, MJ, Schuster, F, Emans, N, Fischer, R and Schillberg, S (2002) Targeting tryptophan decarboxylase to selected subcellular compartments of tobacco plants affects enzyme stability and in vivo function and leads to a lesion mimic phenotype. Plant Physiology 129: 1160–1169.CrossRefGoogle ScholarPubMed
Dnyansagar, VR and Sudhakaran, IV (1968) Meiotic Studies in Vinca rosea Linn. Cytologia 33: 453–464.Google Scholar
Dnyansagar, VR and Sudhakaran, IV (1970) Induced tetraploidy in Vinca rosea Linn. Cytologia 35: 227–241.CrossRefGoogle Scholar
Dnyansagar, VR and Sudhakaran, IV (1977) Seed development in diploid and tetraploid of Vinca rosea syn. Catharanthus roseus (Lochnera rosea). Proceedings of the National Academy of Sciences, India 43 (Part B): 133–141.Google Scholar
Duge de Bernonville, T, Clastre, M, Besseau, S, Oudin, A, Burlat, V, Glevarec, G, Lanoue, A, Papon, N, Giglioli-Guivarc'h, N, Benoit St-Pierre, B and Courdavault, V (2015) Phytochemical genomics of the Madagascar periwinkle: unravelling the last twists of the alkaloid engine. Phytochemistry 113: 9–23.CrossRefGoogle ScholarPubMed
Dutta, A, Batra, J, Pandey-Rai, S, Singh, D, Kumar, S and Sen, J (2005) Expression of terpenoid indole alkaloid biosynthetic pathway genes corresponds to accumulation of related alkaloids in Catharanthus roseus (L.) Don. Planta 220: 376–383.Google Scholar
Dwivedi, S, Singh, M, Singh, AP, Sharma, S, Uniyal, GC and Kumar, S (1999) Genetic variability, heritability and genetic advance foralkaloid yield attributing traits in 26 genotypes of periwinkle Catharanthus roseus . Journal of Medicinal and Aromatic Plant Sciences 21: 320–324.Google Scholar
Dwivedi, S, Singh, M, Singh, AP, Sharma, S, Uniyal, GC and Kumar, S (2000) Assessment of genetic divergence for its purposeful exploitation in periwinkle Catharanthus roseus (Apocynaceae). Journal of Genetics and Breeding 54: 95–99.Google Scholar
El-Sayed, M and Verpoorte, R (2007) Catharanthus terpenoid indole alkaloids: biosynthesis and regulation. Phytochemical Reviews 6: 277–305.CrossRefGoogle Scholar
Fabricant, DS and Farnsworth, NR (2001) The value of plants used in traditional medicine for drug discovery. Environmental Health Perspectives 109 (Supplement 1): 69–75.Google Scholar
Facchini, PJ and De Luca, V (2008) Opium poppy and Madagascar periwinkle: model non-model systems to investigate alkaloid biosynthesis in plants. Plant Journal 54: 763–784.Google Scholar
Flory, WS Jr (1944) Inheritance studies of flower colour in periwinkle. Proceedings of American Society of Horticultural Science 44: 525–526.Google Scholar
Goswami, R, Tyagi, BR, Rani, A, Uniyal, GC and Kumar, S (1996) Colchicine induced autotetraploids in periwinkle Catharanthus roseus . Journal of Medicinal and Aromatic Plant Sciences 18: 38–45.Google Scholar
Guimarães, G, Cardoso, L, Oliveira, H, Santos, C, Duarte, P and Sottomayor, M (2012) Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don. AoB PLANTS 2012: pls002. doi: 10.1093/aobpla/pls002.Google Scholar
Guirimand, G, Guihur, A, Poutrain, P, Héricourt, F, Mahroug, S, St-Pierre, B, Burlat, V, and Courdavault, V (2011) Spatial organization of the vindoline biosynthetic pathway in Catharanthus roseus . Journal of Plant Physiology 168: 549–557.CrossRefGoogle ScholarPubMed
Gupta, S, Pandey-Rai, S, Srivastava, S, Naithani, SC, Prasad, M and Kumar, S (2007) Construction of genetic linkage map of the medicinal and ornamental plant Catharanthus roseus . Journal of Genetics 86: 259–268.CrossRefGoogle ScholarPubMed
Henikoff, S, Till, BJ and Comai, L (2004) TILLING. Traditional mutagenesis meets functional genomics. Plant Physiology 135: 630–636.CrossRefGoogle ScholarPubMed
Husain, A (1993) Medicinal Plants and their Cultivation. Lucknow, India: Central Institute of Medicinal and Aromatic Plants.Google Scholar
Janaki Ammal, EK and Bezbaruah, HP (1963) Induced teteraploidy in Catharanthus roseus (L.) Don. Proceedings of the National Academy of Sciences, India, Section B 57: 339–342.CrossRefGoogle Scholar
Jhang, T, Gautam, TP, Shukla, S, Fatayal, D, Annula, and Kulkarni, RN (2012) Development of microsatellite marker resource for genome analysis of Catharanthus roseus. In: Proceedings of International Plant Conference on “Molecular Mapping & Marker Assisted Selection”, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, Austria. Vienna (8–11th February 2012) International Plant Conference Association, p 39.Google Scholar
Jia, C-H, Dai, Z-F, Xu, B-Y, Jin, Z-Q, Zhang, L, Chen, Y-Y and Wang, J-B (2008) Analysis of karyotype of Catharanthus roseus (Apocynaceae). Journal of Tropical and Subtropical Botany 16: 169–172.Google Scholar
Johal, GS, Hulbert, SH, and Briggs, SP (1995) Disease lesion mimics of maize: a model for cell death in plants. Bioessays 17: 685–692.Google Scholar
Joshi, RK, Kar, B and Nayak, S (2011) Exploiting EST databases for the mining and characterization of short sequence repeat (SSR) markers in Catharanthus roseus L. Bioinformation 5: 378–81.CrossRefGoogle ScholarPubMed
Julio, E, Laporte, F, Reis, S, Rothan, C and Dorlhac de Borne, F (2008) Reducing the content of nornicotine in tobacco via targeted mutation breeding. Molecular Breeding 21: 369–381.Google Scholar
Knuth, P (1909) Handbook of Flower Pollination, vol. III, Oxford: Clarendon Press.Google Scholar
Krishnan, R (1995) Periwinkle. In: Chadda, KL and Gupta, R (eds) Advances in Horticulture, vol. 11. New Delhi: Malhotra Publishing House, pp. 409–428.Google Scholar
Krishnan, R, Naragund, VR and Vasantha Kumar, T (1979) Evidence for outbreeding in Catharanthus roseus . Current Science 48: 80–82.Google Scholar
Krishnan, R, Chandravadana, MV, Mohan Kumar, GN and Ramachander, PR (1985) Effect of induced autotetrapoidy on alkaloid content and root weight in Catharanthus roseus (L.) G. Don. Herba Hungarica 24: 43–51.Google Scholar
Ku, C, Chung, W-C, Chen, L-L and Kuo, C-H (2013) The Complete plastid genome sequence of Madagascar periwinkle Catharanthus roseus (L.) G. Don: plastid genome evolution, molecular marker identification, and phylogenetic implications in Asterids. PLoS ONE 8: e68518. doi: 10.1371/journal pone.0068518 CrossRefGoogle Scholar
Kulkarni, RN (1984) Relative resistance of diploid tetraploid plants of Catharanthus roseus plants to die back disease. Current Science 53: 577.Google Scholar
Kulkarni, RN (1999) Evidence for phenotypic assortative mating for flower colour n periwinkle. Plant Breeding 118: 561–564.CrossRefGoogle Scholar
Kulkarni, RN and Baskaran, K (2003) Inheritance of resistance to Pythium dieback in the medicinal plant, periwinkle. Plant Breeding 122: 184–187.CrossRefGoogle Scholar
Kulkarni, RN and Baskaran, K (2008) Inheritance of pollen-less anthers and ‘thrum’ and ‘pin’ flowers in periwinkle. Journal of Heredity 99: 426–431.Google Scholar
Kulkarni, RN and Baskaran, K (2013a) From Herkogamy to Cleistogamy – development of cleistogamy in periwinkle. Journal of Heredity 104: 140–148.CrossRefGoogle ScholarPubMed
Kulkarni, RN and Baskaran, K (2013b) Individual and combined effects of genes producing opposite effects on plant height in periwinkle (Catharanthus roseus). Journal of Crop Science and Biotechnology 16: 123–129.Google Scholar
Kulkarni, RN and Baskaran, K (2014) Increasing total leaf alkaloid concentrations in periwinkle (Catharanthus roseus) by combining the macro-mutant traits of two induced leaf mutants (‘necrotic leaf’ and ‘nerium leaf’). Journal of Horticultural Science and Biotechnology 89: 513–518.CrossRefGoogle Scholar
Kulkarni, RN and Ravindra, NS (1988) Resistance to Pythium aphanidrmatum in diploids and induced autotetraploids of Catharanthus roseus . Planta Medica 54: 356–359.Google Scholar
Kulkarni, RN and Ravidra, NS (1997) Integration of host resistance with fungicide in the control of dieback of periwinkle. Tropical Agriculture 74: 321–323.Google Scholar
Kulkarni, RN, Chandrashekar, RS and Dimri, BP (1984) Induced autotetraploidy in Catharanthus roseus: a preliminary report. Current Science 53: 484–486.Google Scholar
Kulkarni, RN, Rajagopal, K, Chandrashekar, RS, Dimri, BP, Suresh, N and Rao, BRR (1987) Performance of diploids and induced autotetraploids of Catharanthus roseus under different levels of nitrogen and plant spacing. Plant Breeding 98: 136–140.CrossRefGoogle Scholar
Kulkarni, RN, Kalra, A and Ravindra, NS (1992) Integration of soil solarization with host resistance in the control of die back and collar rot of periwinkle. Tropical Agriculture 69: 217–222.Google Scholar
Kulkarni, RN, Chandrashekara, RS and Chadrashekara, G (1995) Nitrogen utilization efficiency of diploid and induced autotetraploids strains of periwinkle. Tropical Agriculture 72: 249–251.Google Scholar
Kulkarni, RN, Baskaran, K, Chandrashekara, RS and Kumar, S (1999) Inheritance of morphological traits of periwinkle mutants with modified contents and yields of leaf and root alkaloids. Plant Breeding 118: 71–74.CrossRefGoogle Scholar
Kulkarni, RN, Sreevalli, Y, Baskaran, K and Kumar, S (2001) The mechanism and inheritance of intraflower self-pollination in self-pollinating strains of periwinkle. Plant Breeding 120: 247–250.Google Scholar
Kulkarni, RN, Sreevalli, Y and Baskaran, K (2005a) Allelic genes at two loci govern different mechanisms of intraflower self-pollination in self-pollinating strains of periwinkle. Journal of Heredity 95: 71–77.Google Scholar
Kulkarni, RN, Baskaran, K and Sreevalli, Y (2005b) Genetics of novel corolla colours in periwinkle. Euphytica 144: 101–107.Google Scholar
Kulkarni, RN, Baskaran, K and Sreevalli, Y (2008) Genetics of corolla colour in periwinkle: relationship between genes determining violet, orange-red and magenta corolla. Journal of Applied Horticulture 10: 20–23.Google Scholar
Kulkarni, RN, Baskaran, K, Shyamaprasad, DV and Kulkarni, SS (2009) Individual and combined effects of plant height reducing genes in periwinkle. Euphytica 170: 309–316.Google Scholar
Kumar, S, Chaudhary, S, Kumari, R, Sharma, V and Kumar, AA (2012) Development of improved horticultural genotypes characterized by novel over-flowering inflorescence trait in periwinkle Catharanthus roseus . Proceedings of the National Academy of Sciences, India, Section B, Biological Sciences 82: 399–404.Google Scholar
Kumar, S, Shah, S, Garg, V and Bhatia, S (2014) Large scale in-silico identification and characterization of simple sequence repeats (SSRs) from de novo assembled transcriptome of Catharanthus roseus (L.) G. Don. Plant Cell Reports 33: 905–918.CrossRefGoogle ScholarPubMed
Kumari, R, Sharma, V, Sharma, V and Kumar, S (2013a) Pleiotropic phenotypes of the salt-tolerant and cytosine hypomethylated leafless inflorescence, evergreen dwarf and irregular leaf lamina mutants of Catharanthus roseus possessing Mendelian inheritance. Journal of Genetics 92: 369–394.Google Scholar
Kumari, R, Yadav, G, Sharma, V, Sharma, V and Kumar, S (2013b) Cytosine hypomethylation at CHG and CHH sites in the pleiotropic mutants of Mendelian inheritance in Catharanthus roseus . Journal of Genetics 92: 499–511.Google Scholar
Lahlou, M (2013) The success of natural products in drug discovery. Pharmacology & Pharmacy 4: 17–31.Google Scholar
Lavania, UC (2005) Genomic and ploidy manipulation for enhanced production of phyto-pharmaceuticals. Plant Genetic Resources: Characterization and Utilization 3: 170–177.Google Scholar
Lavania, UC, Srivastava, S, Lavania, S, Basu, S, Misra, NK and Mukai, Y (2012) Autopolyploidy differentially influences body size in plants, but facilitates enhanced accumulation of secondary metabolites, causing increased cytosine methylation. Plant Journal 71: 539–549.CrossRefGoogle ScholarPubMed
Levy, A (1982) Natural and induced genetic variation in the biosynthesis of alkaloids and other secondary metabolites. In: Improvement of Oil Seed and Industrial Crops by Induced Mutations. Vienna, Austria: IAEA, pp. 213–222.Google Scholar
Levy, A, Milo, J, Ashri, A and Palevitch, D (1983) Heterosis and correlation analysis of the vegetative components and ajmalicine content in the roots of the medicinal plant – Catharanthus roseus (L.) G. Don. Euphytica 32: 557–564.Google Scholar
Lin, X, Zhou, Y, Zhang, J, Lu, X, Zhang, F, Shen, Q, Wu, S, Chen, Y, Wang, T and Tang, K (2011) Enhancement of artemisinin content in tetraploid Artemisia annua plants by modulating the expression of genes in artemisinin biosynthetic pathway. Biotechnology and Applied Biochemistry 58: 50–57.Google Scholar
Mackay, JF, Wright, CD and Bonfiglioli, RG (2008) A new approach to varietal identification in plants by microsatellite high resolution melting analysis: application to the verification of grapevine and olive cultivars. Plant Methods 4: 8.Google Scholar
Madani, H, Hosseini, B, Dehghan, E and Rezaei-chiyaneh, E (2015) Enhanced production of scopolamine in induced autotetraploid plants of Hyoscyamus reticulatus L. Acta Physiologiae Plantarum 37: 55. doi: 10.1007/s11738–015–1795-x CrossRefGoogle Scholar
Magnotta, M, Murata, J, Chen, J and De Luca, V (2006) Identification of a low vindoline accumulating cultivar of Catharanthus roseus (L.) G. Don by alkaloid and enzymatic profiling. Phytochemistry 67: 1758–1764.Google Scholar
Matsuura, HN, Rau, MR and Fett-Neto, AG (2014) Oxidative stress and production of bioactive monoterpene indole alkaloids: biotechnological implications. Biotechnology Letters 36: 191–200.CrossRefGoogle ScholarPubMed
Mba, C (2013) Induced mutations unleash the potentials of plant genetic resources for food and agriculture. Agronomy 3: 200–231.Google Scholar
McCallum, CM, Comai, L, Greene, EA and Henikoff, S (2000) Targeted screening for induced mutations. Nature Biotechnology 18: 455–457.CrossRefGoogle ScholarPubMed
Micke, A (1988) Genetic improvement of grain legumes using induced mutations: an overview. In: Improvement of Grain Legume Production using Induced Mutations. Vienna, Austria: IAEA, pp. 1–51.Google Scholar
Miettinen, K, Dong, L, Navrot, N, Schneider, T, Burlat, V, Pollier, J, Woittiez, L, van der Krol, S, Lugan, R, Ilc, T, Verpoorte, R, Oksman-Caldentey, KM, Martinoia, E, Bouwmeester, H, Goossens, A, Memelink, J and Werck-Reichhart, D (2014) The seco-iridoid pathway from Catharanthus roseus . Nature Communications 5, Article number: 3606. doi: 10.1038/ncomms4606.Google ScholarPubMed
Milo, J, Levy, A, Akavia, N, Ashri, A, and Palevitch, D (1985) Inheritance of corolla colour and anthocyanin pigments in periwinkle Catharanthus roseus (L) G. Don. Zeitschrift für Pflanzenzüchtung 95: 352–360.Google Scholar
Mishra, P and Kumar, S (2001) A monogenic recessive mutant with precocious in situ pollen germination in periwinkle Catharanthus roseus . Journal of Medicinal and Aromatic Plant Sciences 22/4A &23/1A: 277–279.Google Scholar
Mishra, P and Kumar, S (2003) Manifestation of heterostyle character by induction of recessive hsf mutation responsible for thrum type herkogamous flowers in Catharanthus roseus . Journal of Medicinal and Aromatic Plant Sciences 25: 2–7.Google Scholar
Mishra, P, Uniyal, GC, Sharma, S and Kumar, S (2001) Pattern of diversity for morphological and alkaloid yield related traits among the periwinkle Catharanthus roseus accessions collected from in and around Indian subcontinent. Genetic Resources and Crop Evolution 48: 273–286.Google Scholar
Mishra, RK, Gangadhar, BH, Yu, JW, Kim, DH and Park, SP (2011) Development and characterization of EST based SSR markers in Madagascar periwinkle (Catharanthus roseus) and their transferability in other medicinal plants. Plant Omics Journal 4: 154–162.Google Scholar
Mohan Kumar, GN (1980) Comparative studies on growth and alkaloid of autotetraploids and their diploid progenitors in Catharanthus roseus (L.) G. Don . M. Sc. (Hort) Thesis, University of Agricultural Sciences, Bangalore, India.Google Scholar
Moreno, PRH, van der Heijden, R and Verpoorte, R (1995) Cell and tissue cultures of Catharanthus roseus (L.) G. Don: a literature survey II. Updating from 1988 to 1993. Plant Cell Tissue and Organ Culture 42: 1–25.Google Scholar
Moudi, M, Go, R, Yien, CYS, Nazre, M (2013) Vinca alkaloids. International Journal of Preventive Medicine 4: 1231–1235.Google Scholar
Murata, J, Bienzle, D, Brandle, JE, Sensen, CW and De Luca, V (2006) Expressed sequence tags from Madagascar periwinkle (Catharanthus roseus). FEBS Letters 580: 4501–4507.CrossRefGoogle ScholarPubMed
Nef, C, Rio, B and Chrestin, H (1991) Induction of catharanthine synthesis and stimulation of major indole alkaloids production by Catharanthus roseus cells under non-growth altering treatment with Pythium vexans extracts. Plant Cell Reports 10: 26–29.Google Scholar
Noble, RL, Beer, CT and Cutts, JH (1958) Role of chance observations in chemotherapy: Vinca rosea . Annals of the New York Academy of Sciences 76: 882–894.Google Scholar
Ou, X, Long, L, Wu, Y, Yu, Y, Lin, X, Qi, X and Liu, B (2010) Spaceflight-induced genetic and epigenetic changes in the rice (Oryza sativa L.) genome are independent of each other. Genome 53: 524–32.Google Scholar
Pareek, SK, Singh, S, Srivastava, VK, Mandal, S, Maheshwari, ML and Gupta, R (1981) Advances in periwinkle cultivation. Indian Farming 31: 18–21.Google Scholar
Palazon, J, Cusido, RM, Gonzalo, J, Bonfill, M, Morales, C and Pinol, MT (1998) Relation between the amount of rolC gene product and indole alkaloid accumulation in Catharanthus roseus transformed root cultures. Journal of Plant Physiology 153: 712–718.Google Scholar
Pasquali, G, Porto, DD and Fett-Neto, AG (2006) Metabolic engineering of cell cultures versus whole plant complexity in production of bioactive monoterpene indole alkaloids: recent progress related to old dilemma. Journal of Bioscience and Bioengineering 101: 287–296.CrossRefGoogle ScholarPubMed
Pathirana, R (2011) Plant mutation breeding in agriculture. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 6: No. 032: doi: 10.1079/PAVSNNR20116032m.CrossRefGoogle Scholar
Pezzuto, JM (1997) Plant-derived anticancer agents. Biochemical Pharmacology 53: 121–133.Google Scholar
Rai, SP and Kumar, S (2001) Heterocarpus flowers resulting from a recessive mutation in periwinkle Catharanthus roseus . Current Science 80: 1581–1584.Google Scholar
Rai, SP, Luthra, R and Kumar, S (2001) Differential expression of proteins in monogenic salt resistant mutants with and without thermal stress in periwinkle Catharanthus roseus . Journal of Medicinal and Aromatic Plant Sciences 22/4A &23/1A: 287–290.Google Scholar
Rai, SP, Luthra, R and Kumar, S (2003) Salt-tolerant mutants in glycophytic salinity response (GSR) genes in Catharanthus roseus . Theoretical and Applied Genetics 106: 221–230.Google Scholar
Rani, N and Kumar, K (2011) Karyomorphological studies in the genus Catharanthus . Indian Journal of Genetics and Plant Breeding 71: 55–60.Google Scholar
Rendle, AB (1971) The Classification of Flowering Plants, II. Cambridge: Cambridge University Press.Google Scholar
Roepke, J, Wu, M, Salim, V, Thamm, AMK, Murata, J, Ploss, K, Boland, W and De Luca, V (2010) Vinca drug components accumulate exclusively in leaf exudates of Madagascar periwinkle. Proceedings of the National Academy of Sciences of the United States of America 107: 15287–15292.Google Scholar
Ross, IA (1999) Medicinal Plants of the World: Chemical Constituents, Traditional and Modern Medicinal Uses. New Jersey: Humana Press.Google Scholar
Roychowdhury, R and Tah, J (2013) Mutagenesis – a potential approach for crop improvement. In: Hakeem, KR, Ahmad, P and Ozturk, M (eds) Crop Improvement: New Approaches and Modern Techniques. USA: Springer, pp. 149–187.Google Scholar
Saika, H, Oikawa, A, Matsuda, F, Onodera, H, Saito, K, and Toki, S (2011) Application of gene targeting to designed mutation breeding of high-tryptophan rice. Plant Physiology 156: 1269–1277.CrossRefGoogle ScholarPubMed
Saiman, MZ (2014) Terpenoids and terpenoid indole alkaloids in Catharanthus roseus cell suspension cultures. http://hdl.handle.net/1887/29812 Google Scholar
Salim, V and De Luca, V (2013) Towards complete elucidation of monoterpene indole alkaloid biosynthesis pathway: Catharanthus roseus as a pioneer system. In: Giglioli-Guivarc'h, N (ed.) Advances in Botanical Research Volume 68, New Light on Alkaloid Biosynthesis and Future Prospects . Amsterdam, The Netherlands: Academic Press, pp. 1–37.Google Scholar
Salim, AA, Chin, Y-W and Kinghorn, AD (2008) Drug discovery from plants. In: Ramawat, KG, Merillon, JM (eds) Bioactive Molecules and Medicinal Plants. New York: Springer, pp. 1–25.Google Scholar
Schippmann, U, Cunningham, AB and Leaman, DJ (2003) Impact of cultivation and gathering of medicinal plants on biodiversity: global trends and issues. In: Biodiversity and the Ecosystem Approach in Agriculture, Forestry and Fisheries Satellite Event on the Occasion of the Ninth Regular Session of the Commission on Genetic Resources for Food and Agriculture. Rome 12–13 October 2002, pp. 141–167. URL: http://www.fao.org/docrep/005/y4586e/y4586e00.htm Google Scholar
Schmelzer, GH (2007) Catharanthus roseus (L.) G.Don. In: Schmelzer, GH and Gurib-Fakim, A (eds) Prota 11(1): Medicinal plants/Plantes médicinales 1. [CD-Rom]. PROTA, Wageningen, The Netherlands. URL: http://database.prota.org/PROTAhtml/Catharanthus%20roseus_En.htm Google Scholar
Sevestre-Rigouzzo, M, Nef-Campa, C, Ghesquiere, A and Chrestin, H (1993) Genetic diversity and alkaloid production in Catharanthus roseus, C. trichophyllus and their hybrids. Euphyitca 66: 151–159.Google Scholar
Sharma, V, Chaudhary, S, Srivastava, S, Pandey, R and Kumar, S (2012) Characterization of variation and quantitative trait loci related to terpenoid indole alkaloid yield in a recombinant inbred line mapping population of Catharanthus roseus . Journal of Genetics 91: 49–69.Google Scholar
Shokeen, B, Sethy, NK, Choudhary, S and Bhatia, S (2005) Development of STMS markers from the medicinal plant Madagascar periwinkle [Catharanthus roseus (L.) G. Don.]. Molecular Ecology Notes 5: 818–820.Google Scholar
Shokeen, B, Sethy, NK, Kumar, S and Bhatia, S (2007) Isolation and characterization of microsatellite markers for analysis of molecular variation in the medicinal plant Madagascar periwinkle (Catharanthus roseus (L.) G. Don). Plant Science 172: 441–451.Google Scholar
Shokeen, B, Choudhary, S, Sethy, NK and Bhatia, S (2011) Development of SSR and gene-targeted markers for construction of a framework linkage map of Catharanthus roseus . Annals of Botany 108: 321–336.Google Scholar
Simmonds, NW (1960) Flower colour in Lochnera rosea . Heredity 14: 253–261.Google Scholar
Singh, J (1996) Ajmalicine (Raubasine): a medicinally important alkaloid from Catharanthus roseus (Vinca rosea). In: Handa, SS and Kaul, MK (eds) Supplement to Cultivation and Utilization of Medicinal Plants. Jammu-Tawi, India: Regional Research Laboratory, pp. 199–206.Google Scholar
Singh, D, Rai, SK, Pandey-Rai, S, Srivastava, S, Mishra, K, Sharma, S and Kumar, S (2008) Predominance of the serpentine route in monoterpenoid indole alkaloid pathway of Catharanthus roseus . Proceedings of Indian National Science Academy 74: 97–109.Google Scholar
Snoeijer, W (2001) International Register of Catharanthus roseus. Leiden: Leiden/Amsterdam Centre for Drug Research, Division of Pharmacognosy.Google Scholar
Sreevalli, Y (2002) Inheritance of some morphological, floral, reproductive and economically important traits in the medicinal plant, periwinkle [Catharanthus roseus (L.) G. Don] . Ph.D. Thesis, Department of Botany, Bangalore, India.Google Scholar
Sreevalli, Y, Baskaran, K, Kulkarni, RN and Kumar, S (2000) Further evidence for the absence of automatic and intra-flower self-pollination in periwinkle. Current Science 79: 1648–1649.Google Scholar
Sreevalli, Y, Kulkarni, RN and Baskaran, K (2002) Inheritance of flower colour in periwinkle: orange-red corolla and white eye. Journal of Heredity 93: 55–58.Google Scholar
Sreevalli, Y, Baskaran, K, and Kulkarni, RN (2003) Inheritance of functional male sterility in the medicinal plant periwinkle. Indian Journal of Genetics and Plant Breeding 63: 365–366.Google Scholar
Stearn, WT (1975) A synopsis of the genus Catharanthus (Apocynaceae). In: Taylor, RW and Farnsworth, NR (eds) The Catharanthus Alkaloids. Botany, Chemistry, Pharmacology, and Clinical Use. New York: Marcel Dekkar, pp. 9–44.Google Scholar
Svoboda, GH (1975) Introduction. In: Taylor, RW and Farnsworth, NR (eds) The Catharanthus Alkaloids. Botany, Chemistry, Pharmacology, and Clinical Use. New York: Marcel Dekkar, pp. 1–7.Google Scholar
Svoboda, GH and Blake, DA (1975) The phytochemistry and pharmacology of Catharanthus roseus (L.) G. Don. In: Taylor, RW and Farnsworth, NR (eds) The Catharanthus Alkaloids. Botany, Chemistry, Pharmacology, and Clinical Use. New York: Marcel Dekkar, pp. 45–83.Google Scholar
Swaminathan, MS (1972) Mutational reconstruction of crop ideotypes. In: Induced Mutations and Plant Improvement. Vienna, Austria: IAEA, pp. 155–170.Google Scholar
Thamm, NK (2014) Induction and characterization of Catharanthus roseus mutant altered in monoterpenoid indole alkaloid biosynthesis. http://hdl.handle.net/10464/5682 Google Scholar
Tyler, VE (1988) Medicinal plant research: 1953–1987. Planta Medica 54: 95–100.CrossRefGoogle ScholarPubMed
Virk, SS, Singh, OS and Bhullar, BS (1988) Assessment of variability for different quantitative characters in periwinkle. Crop Improvement 15: 138–141.Google Scholar
van der Heijden, R, Verpoorte, R and Ten Hoopen, HJG (1989) Cell and tissue cultures of Catharanthus roseus (L.) G. Don: a literature survey. Plant Cell Tissue and Organ Culture 18: 231–280.Google Scholar
van der Heijden, R, Jacobs, DI, Snoeijer, W, Hallard, D and Verpoorte, R (2004) The Catharanthus Alkaloids: Pharmacognosy and Biotechnology. Current Medicinal Chemistry 11: 1241–1253.Google Scholar
Verma, P, Mathur, AK, Srivastava, A and Mathur, A (2011) Emerging trends in research on spatial and temporal organization of terpenoid indole alkaloid pathway in Catharanthus roseus: a literature update. Protoplasma 249: 255–268.Google Scholar
Wang, Y and Li, J (2006) Genes controlling plant architecture. Current Opinion in Biotechnology 17: 123–129.CrossRefGoogle ScholarPubMed
Wink, M, Alfermann, AW, Franke, R, Wetterauer, B, Distl, M, Windhövel, J, Krohn, O, Fuss, E, Garden, H, Mohagheghzadeh, A, Wildi, E and Ripplinger, P (2005) Sustainable bioproduction of phytochemicals by plant in vitro cultures: anticancer agents. Plant Genetic Resources: Characterization and Utilization 3: 90–100.Google Scholar
Wu, C, Bordeos, A, Madamba, RS, Baraoidan, M, Ramos, M, Wang, GL, Leach, JE and Leung, H (2008) Rice lesion mimic mutants with enhanced resistance to diseases. Molecular Genetics and Genomics 279: 605–619.CrossRefGoogle ScholarPubMed
Xin, Z, Wang, ML, Barkley, NA, Burow, G, Franks, C, Pederson, G and Burke, J (2008) Applying genotyping (TILLING) and phenotyping analyses to elucidate gene function in chemically induced sorghum mutant population. BMC Plant Biology 8: 103. doi: 10.1186/147-2229-8-103.Google Scholar
Xing, S-H, Guo, X-B, Wang, Q, Pan, Q-F, Tian, Y-S, Liu, P, Zhao, J-Y, Wang, G-F, Sun, X-F and Tang, K-X (2011) Induction and flow cytometry identification of tetraploids from seed-derived explants through colchicine treatments in Catharanthus roseus (L.) G. Don. Journal of Biomedicine and Biotechnology 2011, Article ID 793198, http://dx.doi.org/10.1155/2011/793198.Google Scholar
Yu, F and De Luca, V (2013) ATP-binding cassette transporter controls leaf surface secretion of anticancer drug components in Catharanthus roseus . Proceedings of the National Academy of Sciences of the United States of America 110: 15830–15835.Google Scholar
Yu, X, Wu, H, Wei, LJ, Cheng, ZL, Xin, P, Huang, CL, Zhang, KP and Sun, YQ (2007) Characteristics of phenotype and genetic mutations in rice after spaceflight. Advances in Space Research 40: 528–534.CrossRefGoogle Scholar
Zhao, J and Verpoorte, R (2007) Manipulating indole alkaloid production by Catharanthus roseus cell cultures in bioreactors: from biochemical processing to metabolic engineering. Phytochemical Reviews 6: 435–457.Google Scholar
Zhao, J, Hu, Q, Guo, Y-Q and Zhu, WH (2001) Effects of stress factors, bioregulators, and synthetic precursors on indole alkaloid production in compact callus clusters cultures of Catharanthus roseus . Applied Microbiology and Biotechnology 55: 693–698.Google Scholar
Zhao, L, Sander, GW and Shanks, JV (2013) Perspectives of the metabolic engineering of terpenoid indole alkaloids in Catharanthus roseus hairy roots. Advances in Biochemical Engineering/Biotechnology 134: 23–54.Google Scholar
Zárate, R and Verpoorte, R (2007) Strategies for the genetic modification of the medicinal plant Catharanthus roseus (L.) G. Don. Phytochemical Reviews 6: 475–491.Google Scholar
Zhou, M-L, Shao, J-R and Tang, Y-X (2009) Production and metabolic engineering of terpenoid indole alkaloids in cell cultures of the medicinal plant Catharanthus roseus (L.) G. Don (Madagascar periwinkle). Biotechnology and Applied Biochemistry 52: 313–323.Google Scholar
Zhu, X, Zeng, X, Sun, C and Chen, S (2014) Biosynthetic pathway of terpenoid indole alkaloids in Catharanthus roseus . Frontiers of Medicine 8: 285–293.Google Scholar
Zonneveld, BJM, Leitch, IJ and Bennett, MD (2005) First nuclear DNA amount in more than 300 Angiosperms. Annals of Botany 96: 229–244.CrossRefGoogle ScholarPubMed
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