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Circadian clock and photoperiodic flowering genes in adzuki bean (Vigna angularis [Willd.] Ohwi & H. Ohashi)

Published online by Cambridge University Press:  16 July 2014

Moon Young Kim ,
Yang Jae Kang ,
Taeyoung Lee  and
Suk-Ha Lee
Moon Young Kim
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul151-921, Republic of Korea Plant Genomics and Breeding Institute, Seoul National University, Seoul151-921, Republic of Korea
Yang Jae Kang
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul151-921, Republic of Korea Plant Genomics and Breeding Institute, Seoul National University, Seoul151-921, Republic of Korea
Taeyoung Lee
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul151-921, Republic of Korea
Suk-Ha Lee*
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul151-921, Republic of Korea Plant Genomics and Breeding Institute, Seoul National University, Seoul151-921, Republic of Korea
*
* Corresponding author. E-mail: sukhalee@snu.ac.kr
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Abstract

Adzuki bean (Vigna angularis [Willd.] Ohwi & H. Ohashi) is one of the most important legume crops cultivated in East Asia and northern South Asia. Despite its agronomic importance, the lack of available sequence information has made it difficult to improve important agronomic traits. In the present study, we performed de novo assembly of transcript sequences produced by short-read sequencing to construct 59,860 full-length protein-coding sequences in adzuki bean. These genes were subjected to a BLASTP search to identify putative homologues of the 84 Arabidopsis genes involved in the circadian clock and photoperiodic flowering pathway. A large proportion of these Arabidopsis genes were found to be conserved in adzuki bean. However, there were no homologues of six genes including FLOWER LOCUS D (FD) and LEAFY (LFY). Furthermore, the phylogenetic relationships of 25 highly homologous matches to CONSTANS (CO) or CONSTANS-LIKE (COL) of Arabidopsis indicated the lack of a CO orthologue in adzuki bean. FLOWER LOCUS T (FT) and its homologues were found to have two homologous counterparts in adzuki bean. This study provides primary genetic resources that may be useful for producing adzuki bean with improved flowering and fruiting performance in response to environmental changes.

Keywords

adzuki beancircadian clockde novo assemblyfull-length cDNAphotoperiodic flowering pathway
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S49 - S53
Copyright
Copyright © NIAB 2014 

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Table S1

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Supplementary material: File

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Table S2

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Table S3

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