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Cloning and characterization of γ-glutamylcysteine synthetase in the salt- and oxidative stress-tolerant wild tomato species Solanum pennellii under abiotic stresses

Published online by Cambridge University Press:  13 April 2016

Waseim Barriah ,
Naim Najami  and
Hilal Zaid
Waseim Barriah*
Affiliation:
Department of Life Sciences, Ben Gurion University of the Negev, Beer Sheva 84105, Israel Qasemi Research Center, Al-Qasemi Academy, P.O. Box 124, Baqa El-Gharbia 30100, Israel
Naim Najami
Affiliation:
Institute of Applied Research, Affiliated with University of Haifa, The Galilee Society, P.O. Box 437, Shefa-Amr 20200, Israel The Academic Arab College for Education, 22 Hachashmal St., P.O. Box 8349, Haifa 33145, Israel
Hilal Zaid*
Affiliation:
Qasemi Research Center, Al-Qasemi Academy, P.O. Box 124, Baqa El-Gharbia 30100, Israel Faculty of Arts and Sciences, Arab American University Jenin, P.O. Box 240, Jenin, Palestine
*
*Corresponding authors. E-mail: barriah@post.bgu.ac.il; hilalz@qsm.ac.il
*Corresponding authors. E-mail: barriah@post.bgu.ac.il; hilalz@qsm.ac.il
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Abstract

The wild species of tomato Solanum pennellii (Lpa) is more tolerant to salt-induced oxidative stress than the cultivated species Solanum lycopersicum (Lem), due to the increase of several antioxidative metabolites and enzymes in this species under stress. The increase of reduced glutathione (GSH), one of these metabolites, in NaCl-treated Lpa, is due at least partly to the elevation of γ-glutamylcysteine synthetase (γ-ECS). Introgression line IL 8–3, which was found to include the Lpa orthologue of the γ-ECS gene (Lpa γ-ECS) in Lem's genetic background, was used to assign this gene to chromosome 8 and to assess its relative contribution to the effective antioxidative response of Lpa to stress. The growth of IL 8–3 and Lem plants responded similarly to NaCl and cadmium (Cd) stresses. In both genotypes, GSH and H2O2 levels responded also similarly to NaCl stress. NaCl and Cd stresses affected similarly the transcription of the γ-ECS gene in leaves of both IL 8–3 and Lpa plants. The effect of buthionine sulfoximine (BSO), a competitive inhibitor of the γ-ECS enzyme, on γ-ECS transcription was also similar in these two genotypes. Taken together, these results suggest that γ-ECS orthologues differ mainly in the regulation of their transcription and not at the post-transcriptional or translational levels. The mutation(s) led to these differences in the response of the two orthologues to the salinity and heavy metal stresses are expected to occur in a cis-regulatory element(s) located relatively close to γ-ECS.

Keywords

γ-glutamylcysteine synthetase (γ-ECS)glutathioneintrogression linesoxidative stressreactive oxygen species (ROS)
Type
Research Article
Information
Plant Genetic Resources , Volume 15 , Issue 5 , October 2017 , pp. 453 - 460
Copyright
Copyright © NIAB 2016 

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