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Collection of mutants for functional genomics in the legume Medicago truncatula

Published online by Cambridge University Press:  24 May 2011

O. Calderini ,
M. Carelli ,
F. Panara ,
E. Biazzi ,
C. Scotti ,
A. Tava ,
A. Porceddu  and
S. Arcioni
O. Calderini*
Affiliation:
CNR – Istituto di Genetica Vegetale, Perugia, Italy
M. Carelli
Affiliation:
C.R.A. – Centro per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy
F. Panara
Affiliation:
CNR – Istituto di Genetica Vegetale, Perugia, Italy
E. Biazzi
Affiliation:
C.R.A. – Centro per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy
C. Scotti
Affiliation:
C.R.A. – Centro per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy
A. Tava
Affiliation:
C.R.A. – Centro per le Produzioni Foraggere e Lattiero-Casearie, Lodi, Italy
A. Porceddu
Affiliation:
Dipartimento Di Scienze Agronomiche e Genetica Vegetale Agraria, Università degli Studi di Sassari, Sassari, Italy
S. Arcioni
Affiliation:
CNR – Istituto di Genetica Vegetale, Perugia, Italy
*
*Corresponding author. E-mail: ornella.calderini@igv.cnr.it
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Abstract

We have established mutant collections of the model species Medicago truncatula according to current protocols. In particular, we used a transposon (Tnt1) tagging method and an ethyl methanesulfonate (EMS) mutagenesis approach (TILLING). The collections were subjected to both forward and reverse genetics screenings, and several mutants were isolated that affect plant traits (e.g. shoot, root developments, flower morphology, etc.) and also biosynthetic pathways of secondary compounds (saponins and tannins). Genes responsible for some of the mutations were cloned and further characterized.

Keywords

Medicago truncatulamutantsTransposon taggingTILLING
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 174 - 176
Copyright
Copyright © NIAB 2011

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References

Anè, JM, Zhu, H and Frugoli, J (2008) Recent advances in Medicago truncatula genomics. International Journal of Plant Genomics 2008: 256597.CrossRefGoogle ScholarPubMed
Balestrazzi, A, Gonfalonieri, M, Odoardi, M, Ressegotti, V, Allegro, G, Tava, A and Carbonera, D (2004) A trypsin inhibitor cDNA from a novel source, snail medic (Medicago scutellata L.): cloning and functional expression in response to wounding, herbivore, jasmonic and salicylic acid. Plant Science 167: 337–346.CrossRefGoogle Scholar
Cannon, SB, May, GD and Jackson, SA (2009) Three sequenced legume genomes and many crop species: rich opportunities for translational genomics. Plant Physiology 151: 970–977.CrossRefGoogle ScholarPubMed
d'Erfurth, I, Cosson, V, Eschstruth, A, Lucas, H, Kondorosi, A and Ratet, P (2003) Efficient transposition of the Tnt1 tobacco retrotransposon in the model legume Medicago truncatula. Plant Journal 34: 95–106.CrossRefGoogle ScholarPubMed
Iantcheva, A, Chabaud, M, Cosson, V, Barascud, M, Schutz, B, Primard-Brisset, C, Durand, P, Barker, DG, Vlahova, M and Ratet, P (2009) Osmotic shock improves Tnt1 transposition frequency in Medicago truncatula cv Jemalong during in vitro regeneration. Plant Cell Reports 28: 1563–1572.CrossRefGoogle ScholarPubMed
Porceddu, A, Panara, F, Calderini, O, Molinari, L, Taviani, P, Lanfaloni, L, Scotti, C, Carelli, M, Scaramelli, L, Bruschi, G, Cosson, V, Ratet, P, de Larembergue, H, Duc, G, Piano, E and Arcioni, S (2008) An Italian functional genomic resource for Medicago truncatula. BMC Research Notes 1: 129.CrossRefGoogle ScholarPubMed
Tadege, M, Wen, J, He, J, Tu, H, Kwak, Y, Eschstruth, A, Cayrel, A, Endre, G, Zhao, PX, Chabaud, M, Ratet, P and Mysore, KS (2008) Large-scale insertional mutagenesis using the Tnt1 retrotransposon in the model legume Medicago truncatula. The Plant Journal 54: 335–347.CrossRefGoogle ScholarPubMed
Tadege, M, Wang, TL, Wen, J, Ratet, P and Mysore, KS (2009) Mutagenesis and beyond(Tools for understanding legume biology. Plant Physiology 151: 978–984.CrossRefGoogle ScholarPubMed
Weigel, D, Ahn, JH, Blázquez, MA, Borevitz, JO, Christensen, SK, Fankhauser, C, Ferrándiz, C, Kardailsky, I, Malancharuvil, EJ, Neff, MM, Nguyen, JT, Sato, S, Wang, ZY, Xia, Y, Dixon, RA, Harrison, MJ, Lamb, CJ, Yanofsky, MF and Chory, J (2000) Activation tagging in Arabidopsis. Plant Physiology 122: 1003–1014.CrossRefGoogle ScholarPubMed
Xiao, K, Harrison, MJ and Wang, ZY (2005) Transgenic expression of a novel M. truncatula phytase gene results in improved acquisition of organic phosphorus by Arabidopsis. Planta 222: 27–36.CrossRefGoogle Scholar