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Development and identification of new synthetic T. turgidum–T. monococcum amphiploids

Published online by Cambridge University Press:  03 August 2018

Hongyu Li ,
Xiaojuan Liu ,
Minghu Zhang ,
Zhen Feng ,
Dengcai Liu ,
Michael Ayliffe ,
Ming Hao ,
Shunzong Ning ,
Zhongwei Yuan  and
Zehong Yan
...Show all authors
Hongyu Li
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Xiaojuan Liu
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Minghu Zhang
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Zhen Feng
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Dengcai Liu
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Michael Ayliffe
Affiliation:
CSIRO Agriculture, Box 1600, Clunies Ross Street, Canberra, ACT 2601, Australia
Ming Hao
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Shunzong Ning
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Zhongwei Yuan
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Zehong Yan
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Xuejiao Chen
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Lianquan Zhang*
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
*
*Corresponding author. E-mail: zhanglianquan1977@126.com
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Abstract

Triticum monococcum ssp. monococcum has useful traits for bread wheat improvement. The synthesis of Triticum turgidum–T. monococcum amphiploids is an essential step for transferring genes from T. monococcum into bread wheat. In this study, 264 wide hybridization combinations were done by crossing 60 T. turgidum lines belonging to five subspecies with 83 T. monococcum accessions. Without embryo rescue and hormone treatment, from the 10,810 florets pollinated, 1983 seeds were obtained, with a mean crossability of 18.34% (range 0–89.29%). Many hybrid seeds (90.73%, 923/1017) could germinate and produce plants. A total of 56 new amphiploids (AABBAmAm) were produced by colchicine treatment of T. turgidum × T. monococcum F1 hybrids. The chromosome constitution of amphiploids was characterized by fluorescence in situ hybridization using oligonucleotides probes with different chromosome and sub-chromosome specificities. Sodium dodecyl sulphate polyacrylamide gel electrophoresis analysis indicated that the Glu-A1m-b, Glu-A1m-c, Glu-A1m-d and Glu-A1m-h proteins of T. monococcum were expressed in some amphiploids. Despite resistance reduction in several cases, 45 out of 56 amphiploids exhibited resistance to the current predominant Chinese stripe rust races at both the seedling and adult plant stage. These novel amphiploids provide new germplasm for the potential improvement of bread wheat quality and stripe rust resistance.

Keywords

FISHSDS-PAGEstripe rust resistancequality improvement
Type
Research Article
Information
Plant Genetic Resources , Volume 16 , Issue 6 , December 2018 , pp. 555 - 563
Copyright
Copyright © NIAB 2018 

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