Hostname: page-component-78c5997874-4rdpn Total loading time: 0 Render date: 2024-11-10T08:28:12.376Z Has data issue: false hasContentIssue false
  • English
  • Français

Development and identification of new synthetic T. turgidum–T. monococcum amphiploids

Published online by Cambridge University Press:  03 August 2018

Hongyu Li ,
Xiaojuan Liu ,
Minghu Zhang ,
Zhen Feng ,
Dengcai Liu ,
Michael Ayliffe ,
Ming Hao ,
Shunzong Ning ,
Zhongwei Yuan  and
Zehong Yan
...Show all authors
Hongyu Li
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Xiaojuan Liu
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Minghu Zhang
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Zhen Feng
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Dengcai Liu
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Michael Ayliffe
Affiliation:
CSIRO Agriculture, Box 1600, Clunies Ross Street, Canberra, ACT 2601, Australia
Ming Hao
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Shunzong Ning
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Zhongwei Yuan
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Zehong Yan
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Xuejiao Chen
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
Lianquan Zhang*
Affiliation:
Triticeae Research Institute, Sichuan Agricultural University, 211 Huimin Road, Wenjiang, Chengdu, Sichuan 611130, China
*
*Corresponding author. E-mail: zhanglianquan1977@126.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Triticum monococcum ssp. monococcum has useful traits for bread wheat improvement. The synthesis of Triticum turgidum–T. monococcum amphiploids is an essential step for transferring genes from T. monococcum into bread wheat. In this study, 264 wide hybridization combinations were done by crossing 60 T. turgidum lines belonging to five subspecies with 83 T. monococcum accessions. Without embryo rescue and hormone treatment, from the 10,810 florets pollinated, 1983 seeds were obtained, with a mean crossability of 18.34% (range 0–89.29%). Many hybrid seeds (90.73%, 923/1017) could germinate and produce plants. A total of 56 new amphiploids (AABBAmAm) were produced by colchicine treatment of T. turgidum × T. monococcum F1 hybrids. The chromosome constitution of amphiploids was characterized by fluorescence in situ hybridization using oligonucleotides probes with different chromosome and sub-chromosome specificities. Sodium dodecyl sulphate polyacrylamide gel electrophoresis analysis indicated that the Glu-A1m-b, Glu-A1m-c, Glu-A1m-d and Glu-A1m-h proteins of T. monococcum were expressed in some amphiploids. Despite resistance reduction in several cases, 45 out of 56 amphiploids exhibited resistance to the current predominant Chinese stripe rust races at both the seedling and adult plant stage. These novel amphiploids provide new germplasm for the potential improvement of bread wheat quality and stripe rust resistance.

Keywords

FISHSDS-PAGEstripe rust resistancequality improvement
Type
Research Article
Information
Plant Genetic Resources , Volume 16 , Issue 6 , December 2018 , pp. 555 - 563
Copyright
Copyright © NIAB 2018 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahmed, S, Bux, H, Rasheed, A, Gul Kazi, A, Rauf, A, Mahmood, T and Mujeeb-kazi, A (2014) Stripe rust resistance in Triticum durum–T. monococcum and T. durum–T. urartu amphiploids. Australasian Plant Pathology 43: 109–113.Google Scholar
Aslan, D, Ordu, B and Zencirci, N (2016) Einkorn wheat (Triticum monococcum ssp. monococcum) tolerates cold stress better than bread wheat (Triticum aestivum L.) during germination. Journal of Field Crops Central Research Institute 25: 182–192.Google Scholar
Bhagyalakshmi, K, Vinod, KK, Kumar, M, Arumugachamy, S, Prabhakaran, AJ and Raveendran, TS (2008) Interspecific hybrids from wild × cultivated Triticum crosses – a study on the cytological behaviour and molecular relations. Journal of Crop Science and Biotechnology 11: 257–262.Google Scholar
Brandolini, A, Hidalgo, A and Moscaritolo, S (2008) Chemical composition and pasting properties of einkorn (Triticum monococcum L. subsp. monococcum) whole meal flour. Journal of Cereal Science 47: 599–609.Google Scholar
Cakmak, I, Cakmak, O, Eker, S, Ozdemir, A, Watanabe, N and Braun, HJ (1999) Expression of high zinc efficiency of Aegilops tauschii and Triticum monococcum in synthetic hexaploid wheats. Plant Soil 215: 203–209.Google Scholar
Cao, W, Armstrong, K and Fedak, G (2000) A synthetic zhukovskyi wheat. Wheat Information Service 91: 30–32.Google Scholar
Chhuneja, P, Kaur, S, Garg, T, Ghai, M, Kaur, S, Prashar, M, Bains, NS, Goel, RK, Keller, B, Dhaliwal, HS and Singh, K (2008) Mapping of adult plant stripe rust resistance genes in diploid A genome wheat species and their transfer to bread wheat. Theoretical and Applied Genetics 116: 313–324.Google Scholar
Cox, TS, Harrell, LG, Chen, P and Gill, BS (1991) Reproductive behavior of hexaploid/diploid wheat hybrids. Plant Breeding 107: 105–118.Google Scholar
Dorofeev, VF, Udachin, RA, Semenova, LV, Novikova, MV, Grazhdaninova, OD, Shitova, IP, Merezhko, AF and Filatenko, AA (1987) World Wheat. Agropromizdat: Leningrad (in Russian).Google Scholar
Dvorák, J, Terlizzi, P, Zhang, HB and Resta, P (1993) The evolution of polyploid wheats: identification of the A genome donor species. Genome 36: 21–31.Google Scholar
Gill, RS, Dhaliwal, HS and Multani, DS (1988) Synthesis and evaluation of Triticum durum–T. monococcum amphiploids. Theoretical and Applied Genetics 75: 912–916.Google Scholar
Goncharov, NP, Bannikova, SV and Kawahara, T (2007) Wheat artificial amphiploids involving the Triticum timopheevii genome: their studies, preservation and reproduction. Genetic Resources and Crop Evolution 54: 1507–1516.Google Scholar
Gul Kazi, A, Rasheed, A, Bashir, F, Bux, H, Aziz Napar, A and Mujeeb-Kazi, A (2011) A-genome based diversity status and its practical utilization in wheat. Annual Wheat Newsletter 57: 92–114.Google Scholar
Hussien, T, Bowden, RL, Gill, BS and Cox, TS (1998) Chromosomal locations in common wheat of three new leaf rust resistance genes from Triticum monococcum. Euphytica 101: 127–131.Google Scholar
Kema, GH, Lange, W and van Silfhout, CH (1995) Differential suppression of stripe rust resistance in synthetic wheat hexaploid derived from Triticum turgidum subsp. dicoccoides and Aegilops squarrosa. Phytopathology 85: 425–429.Google Scholar
Knott, DR (2000) Inheritance of resistance to stem rust in Medea durum wheat and the role of suppressors. Crop Science 40: 98–102.Google Scholar
Kostov, D (1936) Investigation of polyploid plants. XI. Amphiploid T. timopheevii zhuk. × T. monococcum l. Doklady Academy of Sciences USSR 1: 32–36 (in Russian).Google Scholar
Li, HY, Li, ZL, Zeng, XX, Zhao, LB, Chen, G, Kou, CL, Ning, SZ, Yuan, ZW, Zheng, YL, Liu, DC and Zhang, LQ (2016) Molecular characterization of different Triticum monococcum ssp. monococcum Glu-A1mx alleles. Cereal Research Communications 44: 444–452.Google Scholar
Li, ZL, Li, HY, Chen, G, Liu, XJ, Kou, CL, Ning, SZ, Yuan, ZW, Hao, M, Liu, DC and Zhang, LQ (2017) Molecular characterization of seven novel Glu-A1mx alleles from Triticum monococcum ssp. monococcum. Cereal Research Communications 45: 647–654.Google Scholar
Ma, H, Singh, RP and Mujeeb-Kazi, A (1997) Resistance to stripe rust in durum wheats, A-genome diploids, and their amphiploids. Euphytica 94: 279–286.Google Scholar
Mather, K (1936) Chromosome behavior in a triploid wheat hybrid. Cell and Tissue Research 23: 117–138.Google Scholar
Megyeri, M, Mikó, P, Molnár, I and Kovács, G (2011) Development of synthetic amphiploids based of Triticum turgidum × T. monococcum crosses to improve the adaptability of cereals. Acta Agronomica Hungarica 59: 267–274.Google Scholar
Megyeri, M, Farkas, A, Varga, M, Kovács, G, Molnár-Láng, M and Molnár, I (2012) Karyotypic analysis of Triticum monococcum using standard repetitive DNA probes and simple sequence repeats. Acta Agronomica Hungarica 60: 87–95.Google Scholar
Megyeri, M, Mikó, P, Farkas, A, Molnár-Láng, M and Molnár, I (2017) Cytomolecular discrimination of the Am chromosomes of Triticum monococcum and the A chromosomes of Triticum aestivum using microsatellite DNA repeats. Journal of Applied Genetics 58: 67–70.Google Scholar
Mikhova, S (1988) Sources of resistance to yellow rust (Puccinia striiformis West.) in the genus Triticum. Rasteniev dni-Nauki 25: 3–8 (English Abstract).Google Scholar
Mikó, P, Megyeri, M, Farkas, A, Molnár, I and Molnár-Láng, M (2015) Molecular cytogenetic identification and phenotypic description of a new synthetic amphiploid, Triticum timococcum (AtAtGGAmAm). Genetic Resources and Crop Evolution 62: 55–66.Google Scholar
Mujeeb-Kazi, A and Hettel, GP (1995) Utilizing wild grass biodiversity in wheat improvement: 15 years of wide cross research at CIMMYT. CIMMYT Research Report 2: 1–140.Google Scholar
Mujeeb-Kazi, A and Kimber, G (1985) The production, cytology and practicality of wide hybrids in the Triticeae. Cereal Research Communications 13: 111–124.Google Scholar
Payne, PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Annual Review of Plant Physiology 38: 141–153.Google Scholar
Plamenov, D, Belchev, I, Kiryakova, V and Spetsov, P (2009) Fungal resistance of Triticum durum–T. monococcum ssp. aegilopoides amphiploid. Journal of Plant Disease and Protection 116: 60–62.Google Scholar
Rouse, M and Jin, Y (2011a) Genetics of resistance to race TTKSK of Puccinia graminis f. sp. tritici in Triticum monococcum. Phytopathology 101: 1418–1423.Google Scholar
Rouse, M and Jin, Y (2011b) Stem rust resistance in A-genome diploid relatives of wheat. Plant Disease 95: 941–944.Google Scholar
Schmolke, M, Mohler, V, Hartl, L, Zeller, FJ, Sai, L and Hsam, K (2012) A new powdery mildew resistance allele at the Pm4 wheat locus transferred from einkorn (Triticum monococcum). Molecular Breeding 29: 449–456.Google Scholar
Sodkiewicz, W (2002) Diploid wheat: Triticum monococcum as a source of resistance genes to preharvest sprouting of triticale. Cereal Research Communications 30: 323–328.Google Scholar
Tang, ZX, Yang, ZJ and Fu, SL (2014) Oligonucleotides replacing the roles of repetitive sequences pAs1, pSc119.2, pTa-535, pTa71, CCS1, and pAWRC.1 for FISH analysis. Journal of Applied Genetics 55: 313–318.Google Scholar
The, TT and Baker, EP (1975) Basic studies relating to the transference of genetic characters from Triticum monococcum L. to hexaploid wheat. Australian Journal of Biological Sciences 28: 189–200.Google Scholar
Tranquilli, G, Cuniberti, M, Gianibelli, MC, Bullrich, L, Larroque, OR, MacRitchie, F and Dubcovsky, J (2002) Effect of Triticum monococcum glutenin loci on cookie making quality and on predictive tests for bread making quality. Journal of Cereal Science 36: 9–18.Google Scholar
Van Slageren, MW (1994) Wild wheats: a monograph of Aegilops L. and Amblyopyrum (Jaub. & Spach) Eig (Poaceae). Wageningen Agricultural University: Wageningen, pp. 88–94.Google Scholar
Watanabe, N, Kobayashi, S and Furuta, Y (1997) Effect of genome and ploidy on photosynthesis of wheat. Euphytica 94: 303–309.Google Scholar
Wellings, C and Bariana, H (2004) Assessment scale for recording stripe rust responses in field trials. Cereal Rust Report Season 2004, Plant Breeding Institute-Cereal Rust Laboratory, University of Sydney 2: 1–2.Google Scholar
Yan, ZH, Wan, YF, Liu, KF, Zheng, YL and Wang, DW (2002) Identification of a novel HMW glutenin subunit and comparison of its amino acid sequence with those of homologous subunits. Chinese Science Bulletin 47: 222–226.Google Scholar
Zaharieva, M and Monneveux, P (2014) Cultivated einkorn wheat (Triticum monococcum L. subsp. monococcum): the long life of a founder crop of agriculture. Genetic Resources and Crop Evolution 61: 677–706.Google Scholar
Zeng, DY, Luo, JT, Li, ZL, Chen, G, Zhang, LQ, Ning, SZ, Yuan, ZW, Zheng, YL, Hao, M and Liu, DC (2016) High transferability of homoeolog-specific markers between bread wheat and newly synthesized hexaploid wheat lines. PLoS ONE 11: e0162847.Google Scholar
Zhang, LQ, Yen, Y, Zheng, YL and Liu, DC (2007) Meiotic restriction in emmer wheat is controlled by one or more nuclear genes that continue to function in derived lines. Sexual Plant Reproduction 20: 159–166.Google Scholar
Zhang, LQ, Yan, ZH, Dai, SF, Chen, QJ, Yuan, ZW, Zheng, YL and Liu, DC (2008) The crossability of Triticum turgidum with Aegilops tauschii. Cereal Research Communications 36: 417–427.Google Scholar
Zhao, LB, Ning, SZ, Yu, JJ, Hao, M, Zhang, LQ, Yuan, ZW, Zheng, YL and Liu, DC (2016) Cytological identification of an Aegilops variabilis chromosome carrying stripe rust resistance in wheat. Breeding Science 66: 522–529.Google Scholar
Supplementary material: File

Li et al. supplementary material

Li et al. supplementary material Figures S1-S4 and Tables S1-S5

Download Li et al. supplementary material(File)
File 1.8 MB