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Development of chloroplast microsatellite markers for identification of Glycyrrhiza species

Published online by Cambridge University Press:  23 October 2018

Kyung Jun Lee ,
Sebastin Raveendar ,
Ji Seon Choi ,
Jinsu Gil ,
Jeong Hoon Lee ,
Yoon-Sup So  and
Jong-Wook Chung
Kyung Jun Lee
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju-54874, Republic of Korea
Sebastin Raveendar
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, Jeonju-54874, Republic of Korea
Ji Seon Choi
Affiliation:
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea
Jinsu Gil
Affiliation:
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea
Jeong Hoon Lee
Affiliation:
Herbal Crop Research Division, Department of Herbal Crop Research, NIHHS, RDA, Eumseong 27709, Republic of Korea
Yoon-Sup So*
Affiliation:
Department of Crop Science, Chungbuk National University, Cheongju 28644, Republic of Korea
Jong-Wook Chung*
Affiliation:
Department of Industrial Plant Science and Technology, Chungbuk National University, Cheongju, Chungbuk 28644, Republic of Korea
*
*Corresponding author. E-mail: yoonsupso@chungbuk.ac.kr and jwchung73@chungbuk.ac.kr
*Corresponding author. E-mail: yoonsupso@chungbuk.ac.kr and jwchung73@chungbuk.ac.kr
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Abstract

Licorice (Glycyrrhiza glabra) is an important medicinal herb and has long been used in traditional medicine for the treatment of several diseases worldwide. Understanding the genetic diversity within Glycyrrhiza species is important for the efficient conservation of these medicinal herbs. In this study, we have developed 20 polymorphic chloroplast microsatellite (cpSSR) markers using the chloroplast genome of G. lepidota. The cpSSR markers were tested on a total of 27 Glycyrrhiza individual plants. The number of alleles per locus ranged from two to eight among the Glycyrrhiza accessions. Overall, the Shannon index (I) for each cpSSR ranged from 0.315 to 1.694, the diversity indices (h) were 0.140–0.793 and the unbiased diversity indices (uh) were 0.145–0.825. In addition, the cpSSR markers were successfully divided and classified the 27 Glycyrrhiza individuals into four groups. The cpSSR markers developed in this study could be used in the assessment of genetic diversity and rapid identification of Glycyrrhiza species.

Keywords

chloroplastgenetic diversityGlycyrrhizamicrosatellites
Type
Short Communication
Information
Plant Genetic Resources , Volume 17 , Issue 1 , February 2019 , pp. 95 - 98
Copyright
Copyright © NIAB 2018 

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Footnotes

†

These authors contributed equally to this work.

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