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Flavonoid content in different legume germplasm seeds quantified by HPLC

Published online by Cambridge University Press:  01 April 2008

M. L. Wang ,
A. G. Gillaspie ,
J. B. Morris ,
R. N. Pittman ,
J. Davis  and
G. A. Pederson
M. L. Wang*
Affiliation:
USDA-ARS, PGRCUUniversity of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
A. G. Gillaspie
Affiliation:
USDA-ARS, PGRCUUniversity of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
J. B. Morris
Affiliation:
USDA-ARS, PGRCUUniversity of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
R. N. Pittman
Affiliation:
USDA-ARS, PGRCUUniversity of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
J. Davis
Affiliation:
Experimental Statistics, University of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
G. A. Pederson
Affiliation:
USDA-ARS, PGRCUUniversity of Georgia, 1109 Experiment Street, Griffin, GA 30223, USA
*
*Corresponding author. E-mail: mingli.wang@ars.usda.gov
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Abstract

Flavonoid content was quantified by high performance liquid chromatography (HPLC) and seed-coat colour was recorded from different legume seeds. Soybean seeds generally contained significantly higher amounts of daidzein (315–354 μg/g), genistein (438–458 μg/g), kaempferol (38–68 μg/g) and total measured flavonoids (892–917 μg/g), while cowpea and peanut seeds contained a significantly higher amount of quercetin (214–280 μg/g and 133–289 μg/g, respectively) than the other legumes tested. Significant variation for flavonoid content existed among and within legume species. Daidzein was significantly correlated with genistein and kaempferol (r = 0.92, P < 0.0001; r = 0.68, P < 0.0001), respectively. Genistein was also significantly correlated with kaempferol (r = 0.84, P < 0.0001). Due to differences in genetic background, no consistent relationship was observed between seed-coat colour and flavonoid content. Variation observed in flavonoid content and seed-coat colour would be useful for legume breeding programmes and consumer use.

Keywords

flavonoid contenthigh performance liquid chromatographylegume germplasmseed-coat colour
Type
Research Article
Information
Plant Genetic Resources , Volume 6 , Issue 1 , April 2008 , pp. 62 - 69
Copyright
Copyright © NIAB 2008

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References

Brim, CA and Burton, JW (1979) Recurrent selection in soybeans. II. Selection for increased percent protein in seeds. Crop Science 19: 494–498.CrossRefGoogle Scholar
Charron, CS, Allen, FL, Johnson, RD, Pantalone, VR and Sams, CE (2005) Correlations of oil and protein with isoflavone concentration in soybean [Glycine max (L.) Merr.]. Journal of Agricultural and Food Chemistry 53: 7128–7135.CrossRefGoogle ScholarPubMed
Dixon, RA and Steele, CL (1999) Flavonoids and isoflavonoids – a gold mine for metabolic engineering. Trends in Plant Science 4: 394–400.CrossRefGoogle Scholar
Eĺias, LG, De Ferńandez, DG and Bressani, R (1979) Possible effects of seed coat polyphenolics on the nutritional quality of bean protein. Journal of Food Science 44: 524–527.CrossRefGoogle Scholar
Islam, FMA, Rengifo, J, Redden, RJ, Basford, KE and Beebe, SE (2003) Association between seed coat polyphenolics (tannins) and disease resistance in common bean. Plant Food for Human Nutrition 58: 285–297.CrossRefGoogle ScholarPubMed
Ma, L and Bliss, FA (1978) Tannin content and inheritance in common bean. Crop Science 18: 201–204.CrossRefGoogle Scholar
McCue, P and Shetty, K (2004) Health benefits of soy isoflavonoids and strategies for enhancement: a review. CRC Critical Reviews in Food Science and Nuturition 44: 361–367.CrossRefGoogle ScholarPubMed
Messina, M (1999) Legumes and soybeans: overview of their nutritional profiles and health effects. American Journal of Clinical Nutrition 70(Suppl): 439s–450s.CrossRefGoogle ScholarPubMed
Mol, J, Grotewold, E and Koes, R (1998) How genes paint flowers and seeds. Trends in Plant Science 3: 212–217.CrossRefGoogle Scholar
Nzaramba, MN, Hale, AL, Scheuring, DC and Miller, JC Jr (2005) Inheritance of antioxidant activity and its association with seed-coat color in cowpea. Journal of the American Society for Horticultural Science 130: 386–391.CrossRefGoogle Scholar
Plahar, WA, Annan, NT and Nti, CA (1997) Cultivar and processing effects on the pasting characteristics, tannin content and protein quality and digestibility of cowpea (Vigna unguiculata). Plant Foods for Human Nutrition 51: 343–356.CrossRefGoogle ScholarPubMed
Ross, JA and Kasum, CM (2002) Dietary flavonoids: bioavailability, metabolic effects, and safety. Annual Review of Nutrition 22: 19–34.CrossRefGoogle ScholarPubMed
Salunkhe, DK, Jadhav, SJ, Kadam, SS and Chavan, JK (1982) Chemical, biochemical, and biological significance of polyphenols in cereal and legumes. CRC Critical Reviews in Food Science and Nutrition 17: 277–305.CrossRefGoogle ScholarPubMed
Todd, JJ and Vodkin, LO (1993) Pigmented soybean (Glycine max) seed coats accumulate proanthocyanidins during development. Plant Physiology 102: 663–670.CrossRefGoogle ScholarPubMed
Wang, ML and Morris, JB (2007) Flavonoid content in seeds of guar germplasm using HPLC. Plant Genetic Resources 5: 96–99.CrossRefGoogle Scholar