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Genetic architecture, physio-biochemical characterization and identification of elite cytoplasmic male sterile (pt-CMS) based combiners in developing antioxidant-rich carrot

Published online by Cambridge University Press:  19 January 2022

Hemant Ghemeray Open the ORCID record for Hemant Ghemeray [Opens in a new window] ,
Raj Kumar ,
T. K. Behera ,
V. K. Sharma ,
Saurabh Singh Open the ORCID record for Saurabh Singh [Opens in a new window] ,
Reeta Bhatia Open the ORCID record for Reeta Bhatia [Opens in a new window]  and
S. S. Dey Open the ORCID record for S. S. Dey [Opens in a new window]
Hemant Ghemeray
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
Raj Kumar
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
T. K. Behera
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
V. K. Sharma
Affiliation:
Division of Germplasm Evaluation, ICAR-National Bureau of Plant Genetic Resources, New Delhi 110012, India
Saurabh Singh*
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
Reeta Bhatia
Affiliation:
Division of Floriculture and Landscaping, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
S. S. Dey*
Affiliation:
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India
*
Author for correspondence: S. S. Dey, E-mail: shyam.iari@gmail.com
Author for correspondence: S. S. Dey, E-mail: shyam.iari@gmail.com
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Abstract

Existence of genetic divergence, appropriate characterization of breeding lines for economically important traits and determining parents with favourable alleles is the crux of crop genetic improvement programmes. This study is the first report of unravelling genetics and potential of petaloid-type cytoplasmic male sterile (pt-CMS) lines in carrot. Ten pt-CMS lines were crossed with 10 inbreds in line × tester mating fashion to generate 100 testcross progenies. Nutritional profiling of the 100 testcrosses progenies along with 20 parental types was carried out for two consecutive years for eight important traits to identify superior combiners. The pooled analysis revealed that the carotenoid content in root is under the genetic control of major genes (oligogenic). The pooled analysis revealed less than unity value of σ2A/D and σgca2/σsca2 for majority of the traits depicting preponderance of non-additive gene effects. The pt-CMS lines KT-28A, Kt-62A, KT-80A and KT-95A were identified as good combiners for carotenoids. The cross combination, KT-98A × KS-50 identified as the best heterotic combiner for CUPRAC and FRAP content over the years. Similarly, the combinations, KT-62A × KS-21, KT-80A × New Kuroda and KT-62A × KS-59 were found promising across the years for developing nutritionally rich F1 hybrids. The interaction analysis among the different antioxidant traits and plant pigments unveiled the scope of simultaneous improvement.

Keywords

Antioxidant traitscombining abilityDaucus carotagenetic architectureheterosispetaloid CMSyear × genotype interaction
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 6 , December 2021 , pp. 484 - 496
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

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