Hostname: page-component-78c5997874-fbnjt Total loading time: 0 Render date: 2024-11-13T03:24:04.608Z Has data issue: false hasContentIssue false
  • English
  • Français

Genetic diversity analyses of Brassica napus accessions using SRAP molecular markers

Published online by Cambridge University Press:  26 July 2013

Riaz Ahmad ,
Farhatullah ,
Carlos F. Quiros ,
Hidayatur Rahman  and
Zahoor Ahmad Swati
Riaz Ahmad
Affiliation:
Department of Plant Sciences, University of California, Davis, CA95616, USA
Farhatullah*
Affiliation:
Department of Plant Breeding and Genetics, The University of Agriculture, Peshawar, Pakistan
Carlos F. Quiros
Affiliation:
Department of Plant Sciences, University of California, Davis, CA95616, USA
Hidayatur Rahman
Affiliation:
Department of Plant Breeding and Genetics, The University of Agriculture, Peshawar, Pakistan
Zahoor Ahmad Swati
Affiliation:
Institute of Biotechnology and Genetic Engineering, The University of Agriculture, Peshawar, Pakistan
*
*Corresponding author. E-mail: drfarhat@aup.edu.pk
Get access Rights & Permissions [Opens in a new window]

Abstract

Knowledge about genetic diversity among Brassica napus cultivars developed for many growing regions and their possible use as potential inbred lines for hybrid seed production is limited. We studied the genetic diversity and relationships among B. napus accessions using Sequence Related Amplified Polymorphism (SRAP) markers, which preferentially amplify open reading frames. A total of 60 spring-type B. napus accessions were screened using 20 SRAP primers, which revealed 162 polymorphic fragments with an average of eight markers per primer combination. Genetic similarity estimates ranged from 40 to 100, which indicated sufficient diversity among the accessions. The majority of the accessions were uniquely identified by the markers with the exception of near-isogenic inbred lines. Cluster analysis displayed five major groups. The first major cluster comprised 23 accessions mostly of Australian origin, whereas the second cluster included 13 accessions mostly of Canadian origin. The accessions in the first and second clusters were identified as maintainers of cytoplasmic male sterility. The two restorer lines R-111 and R-101 along with their corresponding backcross progeny constituted the third cluster. Scandinavian cultivars made the fourth separate cluster. One cultivar Salam and its respective inbred line were the most divergent lines. Variations in the number of markers between open-pollinated cultivars and their respective selfed inbred lines were also observed. The clustering pattern mostly supported their respective pedigree and characteristic traits. Genetic diversity in genetically distinct groups in the tested maintainer and restorer lines can be exploited for hybrid development in B. napus.

Keywords

cytoplasmic male sterilityfertility restorationgenetic diversityoilseed rapeSRAP markers
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue 1 , April 2014 , pp. 14 - 21
Copyright
Copyright © NIAB 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Ahmad, R, Potter, D and Southwick, SM (2004) Genotyping of peach and nectarine cultivars with SSR and SRAP molecular markers. Journal of American Society of Horticulture Science 129: 204–210.Google Scholar
Ahmad, R, Farhatullah, and Carlos, FQ (2011) Inter- and intra-cluster heterosis in spring type oilseed rape (Brassica napus L.) hybrids and prediction of heterosis using SRAP molecular markers. SABRAO Journal of Breeding and Genetics 43: 27–43.Google Scholar
Ahmad, R, Farhatullah, , Khan, RS and Carlos, FQ (2013) Inheritance of fertility restorer gene for cytoplasmic male-sterility in B. napus and identification of closely linked molecular markers to it. Euphytica (May 2013) Advance online publication. DOI: 10.1007/s10681-013-0942-y .Google Scholar
Becker, HC, Engqvist, GM and Karlsson, B (1995) Comparison of rapeseed cultivars and resynthesised lines based on allozyme and RFLP markers. Theoretical and Applied Genetics 91: 62–67.Google Scholar
Budak, H, Shearman, RC, Parmaksiz, I, Gaussoin, RE, Riordan, TP and Dweikat, I (2004) Molecular characterization of buffalograss germplasm using sequence-related amplified polymorphism markers. Theoretical and Applied Genetics 108: 328–334.Google Scholar
Bus, A, Körber, N, Snowdon, RJ and Stich, B (2011) Patterns of molecular variation in a species-wide germplasm set of Brassica napus . Theoretical and Applied Genetics 123: 1413–1423.Google Scholar
Chen, S, Nelson, MN, Ghamkhar, K, Fu, T and Cowling, WA (2008) Divergent patterns of allelic diversity from similar origins: the case of oilseed rape (Brassica napus L.) in China and Australia. Genome 51: 1–10.Google Scholar
Cowling, WA (2007) Genetic diversity in Australian canola and implications for crop breeding for changing future environments. Field Crops Research 104: 103–111.CrossRefGoogle Scholar
Diers, BW and Osborn, TC (1994) Genetic diversity of oilseed Brassica napus germplasm based on restriction fragment length polymorphisms. Theoretical and Applied Genetics 88: 662–668.Google Scholar
Downey, RK and Rimmer, SR (1993) Agronomic improvement in oilseed Brassicas. Advances in Agronomy 50: 1–66.Google Scholar
Doyle, JJ and Doyle, JL (1990) Isolation of plant DNA from fresh tissue. Focus 12: 13–15.Google Scholar
Ferriol, M, Pico, B and Nuez, F (2003) Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theoretical and Applied Genetics 107: 271–282.Google Scholar
Figdore, SS, Kennard, WC, Song, KM, Slocum, MK and Osborn, TC (1988) Assessment of the degree of restriction fragment length polymorphism in brassica. Theoretical and Applied Genetics 75: 833–840.Google Scholar
Grant, I and Beversdorf, WD (1985) Heterosis and combining ability estimates in spring oilseed rape (Brassica napus L.). Canadian Journal of Genetics and Cytology 27: 472–478.Google Scholar
Gehringer, A, Snowdon, R, Spiller, T, Basunanda, P and Friedt, W (2007) New oilseed rape (Brassica napus) hybrids with high levels of heterosis for seed yield under nutrient poor conditions. Breeding Science 57: 315–320.Google Scholar
Guo, DL and Luo, ZR (2006) Genetic relationships of some PCNA persimmons (Diospyros kaki Thunb.) from China and Japan revealed by SRAP analysis. Genetic Resources and Crop Evolution 53: 1597–1603.Google Scholar
Hasan, M, Seyis, F, Badani, AG, Pons-Kuhnemann, J, Friedt, W, Lühs, W and Snowdon, RJ (2006) Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers. Genetic Resource and Crop Evolution 53: 793–802.Google Scholar
Hu, J and Quiros, CF (1991) Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Reports 10: 505–511.Google Scholar
Iñiguez Luy, FL and Federico, ML (2010) The genetics of Brassica napus L. In: Bancroft, I and Schmidt, R (eds) Plant Genetics and Genomics Series: Genetics and Genomics of the Brassicaceae. New York, NY: Springer Science.Google Scholar
Li, G and Quiros, CF (2001) Sequence-related amplified polymorphism (SRAP) a new marker system based on a simple, PCR reaction: its application to mapping, and gene tagging in Brassica. Theoretical and Applied Genetics 103: 455–461.Google Scholar
Lombard, V, Baril, CP, Dubreuil, P, Blouet, F and Zhang, D (2000) Genetic relationship and fingerprinting of rapeseed cultivars by AFLP: consequences for varietal registration. Crop Science 40: 1417–1425.Google Scholar
Mailer, RJ, Scarth, R and Fristensky, B (1994) Discrimination among cultivars of rapeseed (Brassica napus L.) using DNA polymorphisms amplified from arbitrary primers. Theoretical and Applied Genetics 87: 697–704.Google Scholar
Malhi, SS, Brandt, S, Ulrich, D, Lafond, GP, Johnston, AM and Zentner, RP (2007) Comparative nitrogen response and economic evaluation for optimum yield of hybrid and open-pollinated canola. Canadian Journal of Plant Science 87: 449–460.Google Scholar
Nei, M and Li, W (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences USA 79: 5269–5273.CrossRefGoogle Scholar
Palmer, JD, Shields, CR, Cohen, DB and Orton, TJ (1983) Chloroplast DNA evolution and the origin of amphidiploid Brassica species. Theoretical and Applied Genetics 65: 181–189.Google Scholar
Quazi, MH (1988) Interspecific hybrids between Brassica napus L. and B. oleracea L. developed by embryo culture. Theoretical and Applied Genetics 75: 309–318.CrossRefGoogle Scholar
Sernyk, JL (1990) Catalogue of Oilseed Rape Cultivars: 1990 Edition. Madison, WI: Agrigenetics Company.Google Scholar
Sernyk, JL and Stefansson, BR (1983) Heterosis in summer rape (Brassica napus L.). Canadian Journal of Plant Science 63: 407–413.CrossRefGoogle Scholar
Sneath, PHA and Sokal, RR (1973) Numerical Taxonomy: The Principles and Practice of Numerical Classification. San Francisco, CA: Freeman.Google Scholar
Song, K and Osborn, TC (1992) Polyphyletic origins of Brassica napus: New evidence based on organelle and nuclear RFLP analyses. Genome 35: 992–1001.CrossRefGoogle Scholar
Sun, SJ, Gao, W, Lin, SQ, Zhu, J, Xie, BG and Lin, ZB (2006) Analysis of genetic diversity in Ganoderma population with a novel molecular marker SRAP. Applied Microbiology and Biotechnology 72: 537–543.Google Scholar
Turi, NA, Farhatullah, , Malik, AR and Zabta, KS (2012) Genetic diversity in the locally collected Brassica species of Pakistan based on microsatellite markers. Pakistan Journal of Botany 44: 1029–1035.Google Scholar
Wu, X, Chen, B, Lu, G, Wang, H, Xu, K and Guizhan, G (2009) Genetic diversity in oil and vegetable mustard (Brassica juncea) landraces revealed by SRAP markers. Genetic Resources and Crop Evolution 56: 1011–1022.Google Scholar
Supplementary material: File

Ahmad Supplementary Material

Table S1

Download Ahmad Supplementary Material(File)
File 109.1 KB