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Genetic diversity analysis of kenaf (Hibiscus cannabinus L.) using AFLP (amplified fragment length polymorphism) markers

Published online by Cambridge University Press:  10 September 2008

Rouxlene Coetzee ,
Liezel Herselman  and
Maryke T. Labuschagne
Rouxlene Coetzee
Affiliation:
Department of Plant Sciences, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa
Liezel Herselman
Affiliation:
Department of Plant Sciences, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa
Maryke T. Labuschagne*
Affiliation:
Department of Plant Sciences, University of the Free State, PO Box 339, Bloemfontein 9300, South Africa
*
*Corresponding author. E-mail: labuscm.sci@ufs.ac.za
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Abstract

Nineteen kenaf genotypes from Cuba, Taiwan, the USA, El Salvador, Guatemala, Russia, Spain and Indonesia, and three wild types collected in South Africa were analysed for genetic diversity using AFLP analysis. All could be uniquely distinguished from one another, but only a low level of genetic diversity was present. The most distinct accession, Guatemala 4, was 85% similar to all other accessions. The accessions clustered more or less according to known pedigree and/or origin. Two of the three wild types (Hibiscus cannabinus c and H. cannabinus a) clustered separately from the commercial and Russian accessions. One of the wild types, H. cannabinus b clustered with some of the commercial accessions. Commercial accessions in the first subgroup all originated from central and North America, and surrounding islands (Cuba and El Salvador). The Russian accessions are all grouped together. The second subgroup was the only group that contained accessions from different geographical origins.

Keywords

DNA fingerprintingfibremolecular characterization
Type
Research Article
Information
Plant Genetic Resources , Volume 7 , Issue 2 , August 2009 , pp. 122 - 126
Copyright
Copyright © NIAB 2008

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