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Genetic diversity of mungbean (Vigna radiata L.) germplasm in Indonesia

Published online by Cambridge University Press:  16 July 2014

Puji Lestari ,
Sue Kyung Kim ,
Reflinur ,
Yang Jae Kang ,
Nurwita Dewi  and
Suk-Ha Lee
Puji Lestari
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul151-921, Republic of Korea Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, IAARD, Bogor16111, Indonesia
Sue Kyung Kim
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul151-921, Republic of Korea
Reflinur
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul151-921, Republic of Korea Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, IAARD, Bogor16111, Indonesia
Yang Jae Kang
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul151-921, Republic of Korea
Nurwita Dewi
Affiliation:
Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, IAARD, Bogor16111, Indonesia
Suk-Ha Lee*
Affiliation:
Department of Plant Science and Research Institute for Agriculture and Life Sciences, Seoul National University, San 56-1, Sillim-dong, Gwanak-gu, Seoul151-921, Republic of Korea Plant Genomics and Breeding Institute, Seoul National University, Seoul151-921, Republic of Korea
*
* Corresponding author. E-mail: sukhalee@snu.ac.kr
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Abstract

Despite widespread mungbean [Vigna radiata (L.) Wilczek] consumption in Indonesia, few molecular studies have been carried out on accessions and available data are minimal. In this study, we used 30 newly developed simple sequence repeat (SSR) markers designed from the mapped sequence scaffolds of the Korean Sunhwanokdu and Gyeonggijaerae 5 mungbean genomes. These markers were used to examine loci in 83 mungbean accessions collected from diverse geographical areas in Indonesia. A total of 107 alleles were detected among the accessions with 29 polymorphic markers. However, the mean of polymorphic information content (0.33) value and diversity index (0.38) value was indicative of low genetic diversity in this germplasm. The mungbean population structure was not clearly differentiated and the number of subpopulations was unclear. Neighbour-joining tree analysis revealed that the genetic cluster did not reflect the geographical origin of the accessions. Interestingly, the most agriculturally improved varieties were genetically similar to some landraces from one of the main mungbean-producing regions. These newly developed SSR markers could be useful for detecting genetic variability as a basis for establishing a conservation strategy for mungbean germplasm with the aim of enhancing Indonesian breeding programmes.

Keywords

genetic diversityIndonesiamungbeanSSRs
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S91 - S94
Copyright
Copyright © NIAB 2014 

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