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Genetic polymorphisms among and between blast disease resistant and susceptible finger millet, Eleusine coracana (L.) Gaertn.

Published online by Cambridge University Press:  08 March 2016

Dipnarayan Saha Open the ORCID record for Dipnarayan Saha [Opens in a new window] ,
Rajeev Singh Rana ,
Lalit Arya ,
Manjusha Verma ,
M. V. Channabyre Gowda  and
Hari D. Upadhyaya
Dipnarayan Saha*
Affiliation:
Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India
Rajeev Singh Rana
Affiliation:
Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India
Lalit Arya
Affiliation:
Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India
Manjusha Verma
Affiliation:
Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi 110012, India
M. V. Channabyre Gowda
Affiliation:
All India Co-ordinated Small Millets Improvement Project, ICAR, GKVK, Bengaluru-560065, India
Hari D. Upadhyaya
Affiliation:
International Crops Research Institute for the Semi-Arid Tropics, Patancheru, Hyderabad 502324, India
*
*Corresponding author. E-mail: dipsaha72@yahoo.com
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Abstract

Fungal blast disease is one of the major constraints in finger millet production. Breeding for disease resistance in finger millet, needs characterization of genetic polymorphism among and between the resistant and susceptible genotypes. In total, 67 finger millet genotypes, which are resistant or susceptible to fungal blast disease, were analysed using sequence-related amplified polymorphism (SRAP) and simple sequence repeat (SSR) markers to assess genetic variations and select diverse parents. Twelve each of SRAP and SSR primers produced 95.1 and 93.1% polymorphic bands and grouped them into unweighted pair-group method with arithmetic average clusters. Two of the finger millet genotypes, IE 4709 (blast resistant) and INDAF 7 (susceptible) were distinguished as most diverse genotypes as parents. Several genotype-specific bands observed with SSR primers are potential in developing genotype-specific markers. A high genetic diversity within the resistant and susceptible genotypes, rather than between them, was revealed through Nei's gene diversity (h) index and analysis of molecular variance. The finding helps us to understand the extent of genetic polymorphism between blast disease resistant and susceptible finger millet genotypes to exploit in resistance breeding programs.

Keywords

finger milletgenetic variabilityresistant and susceptible genotypessequence related amplified polymorphism (SRAP)simple sequence repeat (SSR)
Type
Research Article
Information
Plant Genetic Resources , Volume 15 , Issue 4 , August 2017 , pp. 355 - 365
Copyright
Copyright © NIAB 2016 

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Footnotes

†

Present address: Division of Crop Improvement, ICAR-Central Research Institute for Jute and Allied Fibres, Barrackpore, Kolkata 700120, India.

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