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Genetic variation of the granule-bound starch synthase I (GBSSI) genes in waxy and non-waxy accessions of Chenopodium berlandieri ssp. nuttalliae from Central Mexico

Published online by Cambridge University Press:  17 March 2015

Verónica Cepeda-Cornejo ,
Douglass C. Brown ,
Guadalupe Palomino ,
Eulogio de la Cruz ,
Melissa Fogarty ,
Peter J. Maughan  and
Eric N. Jellen
Verónica Cepeda-Cornejo
Affiliation:
Department of Plant and Wildlife Sciences, Brigham Young University, 4105 LSB, Provo, UT84602, USA Biotecnología Vegetal, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, Av. Normalistas No. 800, Col. Colinas de la Normal, CP 44270, Guadalajara, Jalisco, Mexico
Douglass C. Brown
Affiliation:
Department of Plant and Wildlife Sciences, Brigham Young University, 4105 LSB, Provo, UT84602, USA
Guadalupe Palomino
Affiliation:
Laboratorio de Citogenética, Jardín Botánico, Instituto de Biología, Universidad Nacional Autónoma de México, 3er. Circuito Exterior, Ciudad Universitaria, Coyoacán 04510, México, D.F., Mexico
Eulogio de la Cruz
Affiliation:
Depto de Biologia, Instituto Nacional de Investigaciones Nucleares, Carretera México-Toluca s/n, La Marquesa, Ocoyoacac, 52750, México
Melissa Fogarty
Affiliation:
Department of Plant and Wildlife Sciences, Brigham Young University, 4105 LSB, Provo, UT84602, USA
Peter J. Maughan
Affiliation:
Department of Plant and Wildlife Sciences, Brigham Young University, 4105 LSB, Provo, UT84602, USA
Eric N. Jellen*
Affiliation:
Department of Plant and Wildlife Sciences, Brigham Young University, 4105 LSB, Provo, UT84602, USA
*
*Corresponding author. E-mail: jellen@byu.edu
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Abstract

Huauzontle (Chenopodium berlandieri ssp. nuttalliae) is a locally important vegetable crop native to the highland valleys of Central Mexico and a potential source of genes for improving its Andean sister crop, quinoa (Chenopodium quinoa). A previous work involving two huauzontle lines identified one waxy genotype that lacked amylose due to mutations in granule-bound starch synthase I (GBSSI), major amylose-synthesis genes with two constituent subgenomes, A and B. We conducted this study to determine the extent of waxy genotypes and cryptic GBSSI mutations in 11 huauzontle accessions or landrace populations extending from Puebla in the southeast to Jalisco in the northwest. This represents one of the first published studies of genetic variation in C. berlandieri ssp. nuttalliae. Accessions were phenotyped for opaque versus translucent seed morphology and their seed starches were stained with Lugol's Stain. In addition, complete or partial GBSSI genes from their A and B genomes were polymerase chain reaction (PCR)-amplified, cloned and sequenced. Seven accessions were either wholly or partially non-waxy while six were either entirely or partially waxy. All waxy accessions carried the same putatively null alleles, designated gbssIa-tp (A-genome) and gbssIb-del (B-genome). The identification of publicly available genotypes carrying gbssIa-tp and their potential use in breeding waxy grain quinoa is discussed.

Keywords

Chenopodium berlandieri ssp. nuttalliaeGBSSIhuauzontlequinoastarchwaxy
Type
Research Article
Information
Plant Genetic Resources , Volume 14 , Issue 1 , March 2016 , pp. 57 - 66
Copyright
Copyright © NIAB 2015 

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