Hostname: page-component-cd9895bd7-lnqnp Total loading time: 0 Render date: 2024-12-26T08:51:52.113Z Has data issue: false hasContentIssue false
  • English
  • Français

In vivo grafting of wild Lens species to Vicia faba rootstocks

Published online by Cambridge University Press:  12 October 2011

Hai Ying Yuan ,
Monika Lulsdorf ,
Abebe Tullu ,
Valar Gurusamy  and
Albert Vandenberg
Hai Ying Yuan
Affiliation:
Crop Development Centre (CDC), University of Saskatchewan, 51 Campus Drive, Saskatoon, Canada SK S7N 5A8
Monika Lulsdorf
Affiliation:
Crop Development Centre (CDC), University of Saskatchewan, 51 Campus Drive, Saskatoon, Canada SK S7N 5A8
Abebe Tullu
Affiliation:
Crop Development Centre (CDC), University of Saskatchewan, 51 Campus Drive, Saskatoon, Canada SK S7N 5A8
Valar Gurusamy
Affiliation:
Canadian Wheat Board, 310-15 Innovation Boulevard, Saskatoon, Canada SK S7N 2X8
Albert Vandenberg*
Affiliation:
Crop Development Centre (CDC), University of Saskatchewan, 51 Campus Drive, Saskatoon, Canada SK S7N 5A8
*
*Corresponding author. E-mail: bert.vandenberg@usask.ca
Get access Rights & Permissions [Opens in a new window]

Abstract

Faba bean (Vicia faba L.) was used as the rootstock for lentil scions to test the feasibility of using in vivo inter-generic grafting techniques as a substitute for root induction and as a tool in lentil genetic improvement. An accession of each of the six wild Lens species was used as the scion in grafts to faba bean breeding line FB50-9 rootstock. Successful grafts were obtained for all species with survival of grafts to seed maturity between 70.7 and 87.7% except for Lens orientalis PI 72735 with 55.3% survival. Days to flower remained the same after grafting, except for scions of L. nigricans PI 72560 and L. orientalis PI 72735 which had a lag phase of 9 and 7 d, respectively. For all six wild species, seed diameter and seed weight were not significantly different between non-grafted controls and scions grafted onto faba bean rootstocks. This simple approach opens the possibility of using in vivo grafting techniques to rescue inter-specific hybrids of lentil. The technique has potential as a useful tool in lentil breeding, as a means of improving seed multiplication rate of rare genetic resources of wild lentil and as a way to reduce the costs of germplasm multiplication of wild lentil species.

Keywords

genetic resourcesgraftingLensrootstockVicia faba L.
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 4 , December 2011 , pp. 543 - 548
Copyright
Copyright © NIAB 2011

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cohen, D, Ladizinsky, G, Ziv, M and Muehlbauer, FJ (1984) Rescue of interspecific Lens hybrids by means of embryo rescue. Plant Cell, Tissue and Organ Culture 3: 343–347.CrossRefGoogle Scholar
Cubero, JI, Perez de la Vega, M and Fratini, R (2009) Origin, phylogeny, domestication and spread. In: Erskine, W, Muehlbauer, FJ, Sarker, A and Sharma, B (eds) The Lentil: Botany, Production and Uses. Wallingford: CABI, pp. 13–33.CrossRefGoogle Scholar
Davies, PA, Lulsdorf, MM, Ahmad, M, et al. (2007) Wild relatives and biotechnological approaches. In: Yadav, SS et al. (ed.) Lentil: An Ancient Crop for Modern Times. Dordrecht: Springer, pp. 225–240.CrossRefGoogle Scholar
Erskine, W and Sarker, A (2004) Lentil Breeding. Encyclopedia of Grain Science, pp. 142–150.CrossRefGoogle Scholar
Fernandez-Aparicio, M, Sillero, JC and Rubiales, D (2009) Resistance to broomrape in wild lentils (Lens spp.). Plant Breeding 128: 266–270.CrossRefGoogle Scholar
Fiala, JV, Tullu, A, Banniza, S, Seguin-Swartz, G and Vandenberg, A (2009) Interspecies transfer of resistance to anthracnose in lentil (Lens culinaris Medic.). Crop Science 49: 825–830.CrossRefGoogle Scholar
Food and Agriculture Organization of the United Nations, FAOSTAT 2009 http://faostat.fao.org/site/339/default.aspx.Google Scholar
Fratini, R and Ruiz, ML (2002) Comparative study of different cytokinins in the induction of morphogenesis in lentil (Lens culinaris Medik). In Vitro Cellular and Developmental Biology – Plant 38: 46–51.CrossRefGoogle Scholar
Garner, RJ (1958) The Grafter's Handbook. London: Faber and Faber Ltd, pp. 150–154.Google Scholar
Gulati, A, Schryer, P and McHughen, A (2001) Regeneration and micrografting of lentil shoots. In Vitro Cellular and Developmental Biology – Plant 37: 798–802.CrossRefGoogle Scholar
Gurusamy, V, Bett, KE and Vandenberg, A (2010) Grafting as a tool in common bean breeding. Canadian Journal of Plant Science 90: 299–304.CrossRefGoogle Scholar
Gurusamy, V, Warkentin, T and Vandenberg, A (2011) Canadian Journal of Plant Science (Accepted).Google Scholar
Khatib, F, Koudsieh, S, Ghazal, B, Barton, JE, Tsujimoto, H and Baum, M (2007) Developing herbicide resistant lentil (Lens culinaris Medikus subsp. culinaris) through Agrobacterium mediated transformation. Arab Journal of Plant Protection 25: 185–192.Google Scholar
Lee, JM (1994) Cultivation of grafted vegetables I. Current status, grafting methods, and benefits. HortScience 29: 235–239.CrossRefGoogle Scholar
Nishimura, R, Hayashi, M, Wu, G-J, Kouchi, H, Imaizumi-Anraku, H, Murakami, Y, Kawasaki, S, Akao, S, Ohmori, M and Nagasawa, M (2002) HAR1 mediates systemic regulation of symbiotic organ development. Nature 420: 426–429.CrossRefGoogle ScholarPubMed
Novák, K (2010) Early action of pea symbiotic gene NOD3 is confirmed by adventitious root phenotype. Plant Science 179: 472–478.CrossRefGoogle ScholarPubMed
Penmetsa, RV, Frugoli, J, Smith, L, Long, SR and Cook, D (2003) Genetic evidence for dual pathway control of nodule number in Medicago truncatula. Plant Physiology 131: 998–1008.CrossRefGoogle Scholar
Polanco, MC and Ruiz, ML (1997) Effect of benzylaminopurine on in vitro and in vivo root development in lentil, Lens culinaris Medik. Plant Cell Reports 17: 22–26.CrossRefGoogle ScholarPubMed
Ramsay, G and Griffiths, DW (1996) Accumulation of vicine and convicine in Vicia faba and V. narbonensis. Phytochemistry 42: 63–67.CrossRefGoogle Scholar
Santa-Cruz, A, Martinez-Rodriguez, MM, Perez-Alfocea, F, Romero-Aranda, R and Bolarin, MC (2002) The rootstock effect on the tomato salinity response depends on the shoot genotype. Plant Science 162: 825–831.CrossRefGoogle Scholar
Sheng, C and Harper, JE (1997) Shoot versus root signal involvement in nodulation and vegetative growth in wild-type and hypernodulating soybean genotypes. Plant Physiology 113: 825–831.CrossRefGoogle ScholarPubMed
Tullu, A, Buchwaldt, L, Lulsdorf, M, Banniza, S, Barlow, B, Sarker, S, Tar'an, B, Warkentin, T and Vandenberg, A (2006) Sources of resistance to anthracnose (Colletotrichum truncatum). Genetic Resources and Crop Evolution 53: 111–119.CrossRefGoogle Scholar
Tullu, A, Banniza, S, Tar'an, B, Warkentin, T and Vandenberg, A (2010) Sources of resistance to ascochyta blight in wild species of lentil (Lens culinaris Medik.). Genetic Resources and Crop Evolution 57: 1053–1063.CrossRefGoogle Scholar
Tullu, A, Diederichsen, A, Suvorova, G and Vandenberg, A (2011) Genetic and genomic resources of lentil: status, use and prospects. Plant Genetic Resources 9: 19–29.CrossRefGoogle Scholar
Venema, JH, Dijk, Boukelien E, Bax, Joze M, van Hasselt, Philip R and Elzenga, J Theo M (2008) Grafting tomato (Solanum lycopersicum) onto the rootstock of a high-altitude accession of Solanum habrochaites improves suboptimal-temperature tolerance. Environmental and Experimental Botany 63: 359–367.CrossRefGoogle Scholar
Williams, JD and McHughen, A (1986) Plant regeneration of the legume Lens culinaris Medik in vitro. Plant Cell, Tissue and Organ Culture 7: 149–153.CrossRefGoogle Scholar