Hostname: page-component-cd9895bd7-jn8rn Total loading time: 0 Render date: 2024-12-27T10:45:32.905Z Has data issue: false hasContentIssue false
  • English
  • Français

Molecular mapping of linked leaf rust resistance and non-glaucousness gene introgressed from Aegilops tauschii Coss. in hexaploid wheat Triticum aestivum L.

Published online by Cambridge University Press:  16 January 2017

Manny Saluja ,
Satinder Kaur ,
Urmil Bansal ,
Subhash Chander Bhardwaj  and
Parveen Chhuneja
Manny Saluja
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, Punjab, India
Satinder Kaur*
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, Punjab, India
Urmil Bansal
Affiliation:
The University of Sydney Plant Breeding Institute-Cobbitty, PMB4011, Narellan, NSW2567, Australia
Subhash Chander Bhardwaj
Affiliation:
Regional Research Station, Indian Institute of Wheat and Barley Research, Flowerdale, Shimla, HP, India
Parveen Chhuneja
Affiliation:
School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana-141004, Punjab, India
*
*Corresponding author. E-mail: satinder.biotech@pau.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

Aegilops tauschii, the D genome donor of wheat, is an invaluable source of genetic variability, which can be utilized for broadening the wheat gene pool. Linked leaf rust resistance and non-glaucousness genes transferred from Ae. tauschii to cultivated wheat variety WH542 were mapped in the present study. Genetic analysis in an F2 population from a BC3 plant derived from the cross Triticum durum cv. PBW114/Ae. tauschii acc. pau14195//4*T. aestivum cv. WH542 revealed monogenic dominant inheritance for both the traits. The leaf rust resistance and the non-glaucousness gene were tentatively named LrT and IwT, respectively. Leaf rust resistance gene exhibited all stage resistance. SSR markers Xbarc124, Xgdm5, Xgdm35, Xcfd51 and EST-derived markers Xcau96 and Xte6 on chromosome 2DS were linked with both genes. Chromosomal assignments of the genes were confirmed by testing linked SSR markers on Chinese Spring nulli-tetrasomics lines. SSR markers Xcau96 (1.6 cM) and Xbarc124 (0.6 cM) flanked LrT and Xgdm35 (4.1 cM) and Xte6 (2.5 cM) flanked non-glaucousness gene. LrT and IwT showed a recombination distance of 3.4 cM. Hence, IwT can be used as an easy to score morphological marker of LrT during its transfer to other glaucous backgrounds.

Keywords

Aegilops tauschiibridging speciesgenetic mappingleaf rust resistancenon-glaucousnesswide hybridization
Type
Research Article
Information
Plant Genetic Resources , Volume 16 , Issue 1 , February 2018 , pp. 82 - 88
Copyright
Copyright © NIAB 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Bansal, M, Kaur, S, Dhaliwal, HS, Bains, NS, Bariana, NS, Chhuneja, P and Bansal, U (2017) Mapping of Aegilops umbelluleta-derived leaf rust and stripe rust resistance loci in wheat. Plant Pathology 66: 38–44.Google Scholar
Bolton, MD, Kolmer, JA and Garvin, DF (2008) Wheat leaf rust caused by Puccinia triticina . Molecular Plant Pathology 9: 563–575.Google Scholar
Chhuneja, P, Garg, T, Kumar, R, Kaur, S, Sharma, A, Bains, NS, Ahuja, M, Dhaliwal, HS and Singh, K (2010) Evaluation of Aegilops tauschii Coss. germplasm for agromorphological traits and genetic diversity using SSR loci. Indian Journal of Genetics 70: 328–338.Google Scholar
Dubcovsky, J, Echaide, M, Giancola, S, Rousset, M, Luo, MC, Joppa, LR and Dvorak, J (1997) Seed-storage-protein loci in RFLP maps of diploid, tetraploid, and hexaploid wheat. Theoretical and Applied Genetics 95: 1169–1180.Google Scholar
Friebe, B, Jiang, J, Raupp, WJ, McIntosh, RA and Gill, BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91: 59–87.Google Scholar
Jenks, MA and Ashworth, EN (1999) Plant epicuticular waxes: function, production, and genetics. Horticultural Reviews 23: 1–68.Google Scholar
Kosambi, DD (1943) The estimation of map distances from recombination values. Annals of Eugenics 12: 172–175.Google Scholar
Liu, Q, Ni, Z, Peng, H, Song, W, Liu, Z and Sun, Q (2007) Molecular mapping of a dominant non-glaucousness gene from synthetic hexaploid wheat (Triticum aestivum L.). Euphytica 155: 71–78.Google Scholar
Lorieux, M (2012) MapDisto: fast and efficient computation of genetic linkage maps. Molecular Breeding 30: 1231–1235.Google Scholar
McIntosh, RA (1998) Breeding wheat for resistance to biotic stresses. Euphytica 100: 19–34.Google Scholar
McIntosh, RA, Yamazaki, Y, Dubcovsky, J, Rogers, J, Morris, C, Somers, J, Appels, R and Devos, KM (2013) Catalogue of gene symbols for wheat. In: KOMUGI-integrated wheat science database at http://www.shigen.nig.ac.jp/wheat/komugi/genes/download.jsp Google Scholar
Nayar, SK, Prashar, M and Bhardwaj, SC (1997) Manual of current techniques in wheat rusts. Research Bull. No.2, 32 pp Regional Station, Flowerdale, Shimla 171002, India.Google Scholar
Peterson, RF, Campbell, AB and Hannah, AE (1948) A diagnostic scale for estimating rust severity on leaves and stem of cereals. Canadian Journal of Research Section C, Botanical Sciences 26: 496–500.Google Scholar
Riederer, M and Schreiber, L (2001) Protecting against water loss: analysis of the barrier properties of plant cuticles. Journal of Experimental Botany 52: 2023–2032.Google Scholar
Röder, MS, Korzun, V, Wendehake, K, Plaschke, J, Tixier, MH, Leroy, P and Ganal, MW (1998) A microsatellite map of wheat. Genetics 149: 2007–2023.CrossRefGoogle ScholarPubMed
Roelfs, AP, Singh, RP and Saari, EE (1992) Rust Diseases of Wheat: Concepts and Methods of Disease Management. Mexico, DF: CIMMYT.Google Scholar
Rosewarne, GM, Singh, RP, Huerta-Espino, J, William, HM, Bouchet, S, Cloutier, S, McFadden, H and Dah, ES (2006) Leaf tip necrosis, molecular markers and b1-proteasome subunits associated with the slow rusting resistance genes Lr46/Yr29. Theoretical and Applied Genetics 112: 500–508.Google Scholar
Saghai-Maroof, MA, Biyashev, RM, Yang, GP, Zhang, Q and Allard, RW (1994) Extraordinarily polymorphic microsatellite DNA in barley: species diversity, chromosomal locations and population dynamics. Proceedings of National Academy of Sciences of the United States of America 91: 5466–5470.Google Scholar
Shah, SJA, Hussain, S, Ahmad, M, Farhatullah, , Ali, I and Ibrahim, M (2011) Using leaf tip necrosis as a phenotypic marker to predict the presence of durable rust resistance gene pair Lr34/Yr18 in wheat. Journal of General Plant Pathology 77: 174–177.Google Scholar
Singh, RP (1992) Association between gene Lr34 for leaf rust resistance and leaf tip necrosis in Wheat. Crop Science 32: 874–878.CrossRefGoogle Scholar
Singh, S, Franks, CD, Huang, L, Brown-Guedira, GL, Marshall, DS, Gill, BS, Fritz, A (2004) Lr41, Lr39 and a leaf rust resistance gene from Aegilops cylindrica may be allelic and are located on wheat chromosome 2DS. Theoretical and Applied Genetics 108: 586–591.Google Scholar
Somers, DJ, Isaac, P and Edwards, K (2004) A high density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 109: 1105–1114.Google Scholar
Sourdille, P, Singh, S, Cadalen, T, Brown-Guedira, G, Gay, G, Qi, L, Gill, B, Dufour, P, Murigneux, A and Bernard, M (2004) Microsatellite-based deletion bin system for the establishment of genetic-physical map relationships in wheat (Triticum aestivum L.). Functional and Integrative Genomics 4: 12–25.Google Scholar
Stakman, EC, Stewart, D and Loegering, WQ (1962) Identification of physiologic races of Puccnia gramnis var. tritici. USDA Agricultural Research Service Report Number E617 Pp 53.Google Scholar
Sun, X, Bai, G and Carver, BF (2009) Molecular markers for wheat leaf rust resistance gene Lr41. Molecular Breeding 23: 311–321.Google Scholar
Tsunewaki, K and Ebona, K (1999) Production of near-isogenic lines of common wheat for glaucousness and genetics basis of the trait clarified by their use. Genes and Genetic Systems 74: 33–41.Google Scholar
Valkoun, JJ (2001) Wheat pre-breeding using wild progenitors. Euphytica 119: 17–23.Google Scholar
Supplementary material: Image

Saluja supplementary material

Saluja supplementary material 1

Download Saluja supplementary material(Image)
Image 84.7 KB
Supplementary material: Image

Saluja supplementary material

Saluja supplementary material 2

Download Saluja supplementary material(Image)
Image 28.4 KB