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Morphological description based on DUS characters and molecular characterization of ‘Sikkim Primitive’ maize: an endangered unique genetic resource

Published online by Cambridge University Press:  10 May 2022

Chandan Kapoor*
Affiliation:
ICAR-Indian Agricultural Research Institute, Pusa, New Delhi, India
Shweta Singh
Affiliation:
ICAR-Indian Institute of Sugarcane Research, Lucknow, UP, India
R. K. Avasthe
Affiliation:
ICAR Research Complex for NEH Region, Sikkim Centre, Gangtok, India
Chandramani Raj
Affiliation:
ICAR-Indian Institute of Sugarcane Research, Lucknow, UP, India
Matber Singh
Affiliation:
ICAR-Indian Institute of Soil and Water Conservation, Dehradun, Uttarakhand, India
Hissay L. Lepcha
Affiliation:
Horticulture Department, Government of Sikkim, Gangtok, India
*
Author for correspondence: Chandan Kapoor, E-mail: chandannaarm@gmail.com

Abstract

‘Sikkim Primitive’ (SP) maize locally known as ‘murali makkai’ in Sikkim is a unique genetic resource exhibiting prolificacy and excellent popping capacity. Status of SP has reached extinction level due to its very small population size and neglected conservation efforts in-situ. In an initial effort to conserve and revive this landrace, characterization and documentation was carried out with 31 morphologically assayed traits recorded at different growth stages along with molecular characterization with simple-sequence repeat (SSR) markers. Plants exhibited prolificacy (5–6 cobs/plant) and excellent popping capacity along with other distinct traits. Plants were tall with thin stem, loose drooping tassel with anthocyanin coloration present at the base of glumes and in brace roots. Cobs were medium sized carrying small seeds with low test weight (87.90 g). A total of 22 SSR markers show amplification in murali makkai with markers bnlg1083, umc1353, umc1128, bnlg1017, bnlg2077, umc2298 and umc2373 amplified unique amplicons ranging from 100 to 800 bp. The characterized set of traits and molecular characterization for murali makkai will facilitate in utilization for genetic improvement and maintenance of genetic purity.

Type
Short Communication
Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press on behalf of NIAB

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