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Phenotypic variants among ethyl methanesulfonate M2 mutant lines in Capsicum annuum

Published online by Cambridge University Press:  16 July 2014

Doyeon Hwang ,
Hee-Jin Jeong ,
Jin-Kyung Kwon ,
Hohyun Kim ,
Si-Yong Kang  and
Byoung-Cheorl Kang
Doyeon Hwang
Affiliation:
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul151-921, Republic of Korea
Hee-Jin Jeong
Affiliation:
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul151-921, Republic of Korea
Jin-Kyung Kwon
Affiliation:
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul151-921, Republic of Korea
Hohyun Kim
Affiliation:
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul151-921, Republic of Korea
Si-Yong Kang
Affiliation:
Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, 1266 Sinjeong-dong, Jeongeup-si, Jeollabuk-do580-185, Republic of Korea
Byoung-Cheorl Kang*
Affiliation:
Department of Plant Science, Plant Genomics and Breeding Institute, and Research Institute for Agriculture and Life Sciences, College of Agricultural Sciences, Seoul National University, 599 Gwanak-ro, Gwanak-gu, Seoul151-921, Republic of Korea
*
* Corresponding author. E-mail: bk54@snu.ac.kr
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Abstract

Induction of mutations using chemical mutagens has proved to be a useful tool in crop improvement and has advantages over transgenic approaches in view of legislative restrictions and intellectual property. Among the chemical mutagens, ethyl methanesulfonate (EMS) has been widely used to generate novel traits. In this study, we constructed an EMS mutant population consisting of 3945 M2 mutant lines using a Korean landrace of Capsicum annuum ‘Yuwol-cho’. In total, 1480 M2 mutant lines were evaluated for novel traits. The mutant lines showed phenotypic variations such as plant growth (small size and dwarfism), development of leaves (variegation, colour and morphological changes), flowers (inflorescence, morphological and organ colour changes), and fruits (morphological and colour changes). Most of these mutant phenotypes were inherited recessively. Many of the mutant phenotypes were unique in pepper, while some were similar to those of known mutants in other plant species. These mutant lines will be useful for the study of gene function in C. annuum.

Keywords

ethyl methanesulfonatemutant linesnovel traitspepper
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S141 - S145
Copyright
Copyright © NIAB 2014 

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