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Polymorphism of waxy proteins in Spanish hulled wheats

Published online by Cambridge University Press:  15 March 2011

C. Guzmán ,
L. Caballero ,
M. V. Gutierrez  and
J. B. Alvarez
C. Guzmán*
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
L. Caballero
Affiliation:
Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible, Consejo Superior de Investigaciones Científicas, Apdo. 4084, ES-14080 Córdoba, Spain
M. V. Gutierrez
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
J. B. Alvarez
Affiliation:
Departamento de Genética, Escuela Técnica Superior de Ingenieros Agrónomos y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, ES-14071 Córdoba, Spain
*
*Corresponding author. E-mail: ge2gugac@uco.es
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Abstract

Hulled wheats are neglected crops that have potential in plant breeding programmes of modern durum and common wheat. Among these wheats, three species were widely cultivated in Spain until the mid 20th century: Triticum monococcum ssp. monococcum (einkorn), Triticum turgidum ssp. dicoccum (emmer) and Triticum aestivum ssp. spelta (spelt). One important aspect of wheat grain quality is starch composition, which is related to the action of waxy proteins. A collection of 536 accessions of Spanish hulled wheats was analyzed for waxy protein composition using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Polymorphism was found for the Wx-A1, Wx-B1 and Wx-D1 proteins, including new and null alleles in the three species. An allelic variant with an electrophoretic mobility not previously described was found in einkorn wheat. In emmer and spelt, some alleles with different mobility were also found. A Wx-B1 null allele was detected in emmer wheat, and null alleles for Wx-A1, Wx-B1 and Wx-D1 were found in spelt wheat. The variations found could be used to enlarge the gene pool available to breeders, and to design new cultivars with different levels of amylose content.

Keywords

amylose contentgenetic polymorphismhulled wheatswaxy proteins
Type
Research Article
Information
Plant Genetic Resources , Volume 9 , Issue 2 , July 2011 , pp. 330 - 333
Copyright
Copyright © NIAB 2011

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