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The utility of ISSRs for the identification of interspecific hybrids between pearl millet (Pennisetum glaucum [L.] R.Br.) × napier grass (Pennisetum purpureum Schumach)

Published online by Cambridge University Press:  23 March 2021

S. S. Jade ,
P. S. Takawale  and
R. A. Bahulikar Open the ORCID record for R. A. Bahulikar [Opens in a new window]
S. S. Jade
Affiliation:
BAIF Development Research Foundation, Central Research Station, Urulikanchan, Dist. Pune 412 202, Maharashtra, India
P. S. Takawale
Affiliation:
BAIF Development Research Foundation, Central Research Station, Urulikanchan, Dist. Pune 412 202, Maharashtra, India
R. A. Bahulikar*
Affiliation:
BAIF Development Research Foundation, Central Research Station, Urulikanchan, Dist. Pune 412 202, Maharashtra, India
*
*Corresponding author. E-mail: Rahul.Bahulikar@baif.org.in
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Abstract

Interspecific hybrids between pearl millet (Pennisetum glaucum) and napier grass (Pennisetum purpureum) give rise to perennial fodder crops characterized by high biomass, broad clumps and good palatability. These hybrids are triploid and developed by hand pollination of napier grass pollen on pearl millet panicles. The progeny shows a high percentage of pearl millet genotype due to self-pollination in the female parent. Identification of hybrids at a young stage based on morphological characters is difficult. DNA-based molecular markers have high discriminating power and were used to assess genetic differences between hybrids and their parents. Genetic diversity was studied in 18 pearl millet × napier grass hybrids along with their parents and two released national checks using inter simple sequence repeat (ISSR) markers. Eight ISSR primers gave rise to 125 bands, of which 120 bands were polymorphic. Polymorphic information content and ISSR primer index ranged from 0.40 to 0.49 and 8.88 to 11.14, respectively. The hybrids showed the presence of unique bands, besides those shared with male and female parents. Female (pearl millet) parents formed a separate group in the dendrogram constructed based on ISSR polymorphism. The male (napier grass) parents formed a separate group along with hybrids, indicating a higher similarity of hybrids with the male parents. Principal component analysis and STRUCTURE analyses showed a similar grouping. The close resemblance of hybrids to the male parents confirmed their interspecific origin. The study revealed that ISSR marker analysis could be a quick and reliable method to identify interspecific hybrids at an early stage of growth.

Keywords

genetic diversityinter-simple sequence repeatinterspecific hybridsmolecular markers
Type
Research Article
Information
Plant Genetic Resources , Volume 19 , Issue 2 , April 2021 , pp. 104 - 111
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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