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Validation of chip grafting inoculation assay to assess the resistance of Solanum species against phytoplasma

Published online by Cambridge University Press:  18 March 2021

Khalid Pervaiz Akhtar Open the ORCID record for Khalid Pervaiz Akhtar [Opens in a new window] ,
Najeeb Ullah  and
Muhammad Yussouf Saleem
Khalid Pervaiz Akhtar*
Affiliation:
Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan
Najeeb Ullah
Affiliation:
Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan
Muhammad Yussouf Saleem
Affiliation:
Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan
*
*Corresponding author. E-mail: kpervaiz_mbd@yahoo.com
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Abstract

Big bud caused by several different phytoplasmas is an emerging threat to tomato production worldwide. The development of resistant varieties would be an effective approach to manage this problem, but it requires an appropriate screening technique. Recently, we have described a simple and efficient chip graft inoculation assay (CGIA) for the first time to screen tomato germplasm against Tomato leaf curl New Delhi virus. The present study was conducted to first validate the CGIA for phytoplasma transmission, then to assess the resistance of 74 genotypes belonging to different Solanum species against 16SrII-D phytoplasma. CGIA success rate and phytoplasma transmission was 100% since all the grafts survived and phytoplasma was detected in these plants using nested polymerase chain reaction. No genotype was found resistant as all the grafted plants showed typical disease symptoms. In addition to phytoplasma transmission, CGIA can be used for better understanding the plant–phytoplasma interactions and biology of phytoplasmas in tomato.

Keywords

chip graftingphyllodyphytoplasmaSolanum spptomato
Type
Short Communication
Information
Plant Genetic Resources , Volume 19 , Issue 2 , April 2021 , pp. 178 - 182
Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of NIAB

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References

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