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Characterization of chitin preparations using powder and film X-ray diffraction methods

Published online by Cambridge University Press:  13 March 2013

Jeffrey R. Deschamps
Jeffrey R. Deschamps*
Affiliation:
Center for Biomolecular Science and Engineering, Naval Research Laboratory, 4555 Overlook Avenue, Washington, District of Columbia 20375
*
a)Author to whom correspondence should be addressed. Electronic mail: deschamps@nrl.navy.mil
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Abstract

Chitin is a natural polysaccharide found in many diverse phyla and almost always occurs in association with protein. In this study the chitin polymer is characterized by X-ray diffraction from its naturally occurring unprocessed state through various steps used in the purification procedure. In addition, the effect of different treatments on the final product is examined. These studies show that native chitin has a characteristic diffraction pattern that is not altered by the mild treatments used to isolate relatively pure chitin. Chitins prepared from different sources exhibit the same characteristic diffraction pattern. In addition, chitin films prepared using non-degrading solvents retain most of the characteristic patterns. De-acylation of chitin to produce chitosan results in large changes to the diffraction pattern. To a very limited extent features present in the diffraction pattern of native chitin can be recovered by re-acylation of chitosan.

Keywords

chitinchitosanpolysaccharideX-ray diffraction
Type
Technical Articles
Information
Powder Diffraction , Volume 28 , Issue 1 , March 2013 , pp. 44 - 48
Copyright
Copyright © International Centre for Diffraction Data 2013 

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