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In Vitro Fermentation of the Non-Starch Polysaccahride Fraction of Tropical Forage Legumes

Published online by Cambridge University Press:  21 November 2017

A.C. Longland ,
R.D. Pilgrim ,
J. Thorpe ,
S.J. Lister ,
P. Morris  and
M.K. Theodorou
A.C. Longland
Affiliation:
AFRC Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB
R.D. Pilgrim
Affiliation:
AFRC Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB
J. Thorpe
Affiliation:
AFRC Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB
S.J. Lister
Affiliation:
AFRC Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB
P. Morris
Affiliation:
AFRC Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB
M.K. Theodorou
Affiliation:
AFRC Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB
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Extract

The fibre fraction (= non-starch polysaccharides; NSP) of tropical forage legumes is a potentially important source of dietary energy. Such legumes, however often contain tannins which can reduce the fermentability of the NSP fraction. In this study, the monosaccharide composition and in vitro digestibility of the NSP fraction of eleven tropical forage legumes varying in condensed tannin content, were investigated.

Milled, lyophilised leaves of 5 species of forage legume from Ethiopia, Sesbania goetzei (SG), Sesbania sesban (SS) Leucaena leucocephala (LL), Acacia cyanophylla (AC), Chamaecytisus palmensis (CP)) and six from Colombia, Calliandra sp. 29400 (C), Dioclea guianensis (DG), Flemingia macrophylla 77409 (FM) Tadehagi sp. 23227 (T23), 13269 (T69) and 13275 (T75)) were used. One gram samples were fermented at 39°C for 7 d under anaerobic conditions in modified Van Soest medium inoculated with bovine rumen fluid (5 replicates per species). At the end of the fermentation period, residues were filtered, washed with distilled water and lyophilised. The NSP content and composition of the original samples and their residues were determined by the method of Englyst and Cummings (1984). The bound, soluble and total condensed tannin contents of the original samples were determined by the method of Terrill et al. (1992).

Type
Tropical Feeds
Information
Proceedings of the British Society of Animal Production (1972) , Volume 1994 , March 1994 , pp. 135
Copyright
Copyright © The British Society of Animal Production 1994

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References

Englyst, H.N. and Cummings, J.H. (1984). Simplified method for the measurement of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars as alditol acetates. Analyst 9: 937–942.Google Scholar
Terrill, T.H., Rowan, A.M., Douglas, G.B. and Barry, T.N. (1992). The determination of extractable, protein-bound and fibre-bound condensed tannins in forage plants, protein concentrate meals and cereal grains. Journal of the Science of Food and Agriculture 28, 1057–1064.Google Scholar