Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-27T14:10:19.745Z Has data issue: false hasContentIssue false
  • English
  • Français

Structural and phylogenetic characterization of human SLURP-1, the first secreted mammalian member of the Ly-6/uPAR protein superfamily

Published online by Cambridge University Press:  01 April 1999

KNUT ADERMANN ,
FRANK WATTLER ,
SIGRID WATTLER ,
GABRIELE HEINE ,
MARKUS MEYER ,
WOLF-GEORG FORSSMANN  and
MICHAEL NEHLS
KNUT ADERMANN
Affiliation:
Lower Saxony Institute for Peptide Research (IPF), Feodor-Lynen-Strasse 31, D-30625 Hannover, Germany
FRANK WATTLER
Affiliation:
Lexicon Genetics Inc., 4000 Research Forest Drive, The Woodlands, Texas 77381
SIGRID WATTLER
Affiliation:
Lower Saxony Institute for Peptide Research (IPF), Feodor-Lynen-Strasse 31, D-30625 Hannover, Germany Lexicon Genetics Inc., 4000 Research Forest Drive, The Woodlands, Texas 77381
GABRIELE HEINE
Affiliation:
Lower Saxony Institute for Peptide Research (IPF), Feodor-Lynen-Strasse 31, D-30625 Hannover, Germany
MARKUS MEYER
Affiliation:
Lower Saxony Institute for Peptide Research (IPF), Feodor-Lynen-Strasse 31, D-30625 Hannover, Germany
WOLF-GEORG FORSSMANN
Affiliation:
Lower Saxony Institute for Peptide Research (IPF), Feodor-Lynen-Strasse 31, D-30625 Hannover, Germany
MICHAEL NEHLS
Affiliation:
Lower Saxony Institute for Peptide Research (IPF), Feodor-Lynen-Strasse 31, D-30625 Hannover, Germany Lexicon Genetics Inc., 4000 Research Forest Drive, The Woodlands, Texas 77381
Get access

Abstract

Members of the Ly-6/uPAR protein family share one or several repeat units of the Ly-6/uPAR domain that is defined by a distinct disulfide bonding pattern between 8 or 10 cysteine residues. The Ly-6/uPAR protein family can be divided into two subfamilies. One comprises GPI-anchored glycoprotein receptors with 10 cysteine residues. The other subfamily includes the secreted single-domain snake and frog cytotoxins, and differs significantly in that its members generally possess only eight cysteines and no GPI-anchoring signal sequence. We report the purification and structural characterization of human SLURP-1 (secreted mammalian Ly-6/uPAR related protein 1) from blood and urine peptide libraries. SLURP-1 is encoded by the ARS (component B)-81/s locus, and appears to be the first mammalian member of the Ly-6/uPAR family lacking a GPI-anchoring signal sequence. A phylogenetic analysis based on the SLURP-1 primary protein structure revealed a closer relationship to the subfamily of cytotoxins. Since the SLURP-1 gene maps to the same chromosomal region as several members of the Ly-6/uPAR subfamily of glycoprotein receptors, it is suggested that both biologically distinct subfamilies might have co-evolved from local chromosomal duplication events.

Keywords

CD59disulfide bondsfrog toxingene locusLy-6 antigensecretionsnake toxinurokinase-type plasminogen activator receptor (uPAR)
Type
Research Article
Information
Protein Science , Volume 8 , Issue 4 , April 1999 , pp. 810 - 819
Copyright
© 1999 The Protein Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)