Periodontitis remains a highly prevalent dental disease worldwide despite general improvements in oral hygiene, such as toothbrushing and interproximal cleaning, and availability of oral health services(Reference Peres, Macpherson and Weyant1). Central to periodontitis is chronic inflammation and progressive destruction of the supporting tissues of the teeth as an excessive immune response to specific bacterial colonisation of dental plaque.
Periodontitis shares numerous risk factors with systemic, chronic diseases like cardiovascular diseases and diabetes, e.g. age, smoking, unhealthy diet, stress and hormonal changes(Reference Chapple, Bouchard and Cagetti2). Preventive measures against periodontitis should comprise periodontal infection control, i.e. gingivitis management(Reference Chapple, Van der Weijden and Doerfer3), and the promotion of healthy lifestyle behaviours. It has been suggested that a healthy, balanced diet can help reduce the risk of periodontitis; recent evidence has highlighted associations between periodontitis and micronutrient deficiencies, high consumption of saturated fats and fermentable carbohydrates(Reference Chapple, Bouchard and Cagetti2,Reference Martinon, Fraticelli and Giboreau4) .
Recently, several studies have attempted to assess the relationship between overall diet and periodontitis(Reference Alhassani, Hu and Li5–Reference Lieske, Moszka and Borof11). Most studies have used hypothesis-driven dietary patterns based on a priori indices, such as indices derived using intake of foods and nutrients correlated with inflammatory biomarkers, e.g. the Dietary Inflammatory Index (mainly nutrient-based)(Reference Li, Chen and Schuller6,Reference Machado, Botelho and Viana7) and anti-inflammatory dietary score (based on nine food groups)(Reference Lieske, Moszka and Borof11), as well as indices that measure adherence to established evidence-based dietary patterns for chronic disease prevention, e.g. the Dietary Approaches to Stop Hypertension and the Mediterranean Diet Score(Reference Altun, Walther and Borof9,Reference Iwasaki, Ennibi and Bouziane12) , plant-based diet indices(Reference Li, Qiu and Goettsch13) and food groups according to the degree of processing(Reference Cassiano, Peres and Motta14). Most studies of a priori indices reported lower adherence to the anti-inflammatory/healthier diet in individuals with periodontitis(Reference Li, Chen and Schuller6,Reference Machado, Botelho and Viana7,Reference Altun, Walther and Borof9,Reference Lieske, Moszka and Borof11,Reference Li, Qiu and Goettsch13,Reference Cassiano, Peres and Motta14) . However, hypothesis-driven approaches neither reflect overall dietary patterns nor consider the correlated structure of the dietary components and nutrients(Reference Zhao, Li and Gao15). Commonly, a priori indices are not developed specifically for the target population; thus, they may not fully reflect the dietary behaviour of this group. Even though data-driven or a posteriori dietary patterns have limited generalisability, empirically derived dietary patterns provide important knowledge that complements the findings of hypothesis-driven methods(Reference Previdelli, de Andrade and Fisberg16). To date, few population-based epidemiological studies on the association between overall diet and periodontitis have focused on a posteriori dietary patterns on the basis of variation in food group intake(Reference Alhassani, Hu and Li5,Reference Wright, McKenna and Nugent8,Reference Alhassani, Hu and Rosner10) and those that do exist rendered inconsistent results. A longitudinal study found no overall association between the Westernised and Prudent dietary patterns, determined by principal component analysis, and self-reported periodontitis(Reference Alhassani, Hu and Li5). A cross-sectional study reported that a dietary pattern rich in salad, fruit and vegetables, poultry, seafood, and plain water or tea, as identified by treelet transformation, was associated with a lower extent of objectively measured periodontitis (i.e. proportion of sites with clinical attachment loss (≥ 3 mm)(Reference Wright, McKenna and Nugent8). Further, it has been suggested that obesity could be an effect modifier in the positive association between the Western dietary pattern and periodontitis, as the association has been found to be significant only in individuals with obesity(Reference Alhassani, Hu and Li5). Another study found no overall association between a pro-inflammatory diet and the risk of self-reported periodontitis, except among non-smokers with obesity, using reduced rank regression, which is an a posteriori method but incorporates prior knowledge about diseases and their pathways(Reference Alhassani, Hu and Rosner10).
Functional dentition (i.e. having ≥ 20 natural teeth) is important for chewing, speech and dental aesthetics. It is well known that periodontitis contributes to extensive or even complete tooth loss, especially among older adults. An association between an anti-inflammatory diet, investigated using the Dietary Inflammatory Index, and fewer missing teeth has recently been demonstrated(Reference Kotsakis, Chrepa and Shivappa17). It is, however, unclear whether empirically derived dietary patterns are associated with extensive tooth loss. Thus, more research is needed to identify which dietary patterns are associated with periodontitis and tooth loss.
The aim of the present study was to explore empirically derived dietary patterns in relation to periodontitis and number of teeth in a general Norwegian population.
Materials and methods
Study population
The Tromsø Study is an ongoing population-based study in Tromsø, Norway. Seven surveys (Tromsø1-Tromsø7) have been conducted between 1974 and 2016, to which total birth cohorts and random population samples have been invited (attendance 65–79 %)(18). Data collection methods comprise questionnaires and interviews, biological sampling and clinical examinations.
Study sample
The present study includes participants from the seventh survey of the Tromsø Study 2015–2016 (Tromsø7)(Reference Hopstock, Grimsgaard and Johansen19). All inhabitants aged ≥ 40 years were invited (n 32 591), and 65 % attended (n 21 083, aged 40–99 years, 53 % women). Of these, a random subsample of 3943 participants attended an oral health examination. Several questionnaires were completed including a thirteen-page semi-quantitative FFQ(Reference Lundblad, Andersen and Jacobsen20), developed and validated at the University of Oslo(Reference Carlsen, Lillegaard and Karlsen21), distributed to the participants at the examination site.
As shown in Figure 1, we excluded participants with missing data on periodontal examination and those with too few teeth (< 2) from categorisation as a periodontitis case. We further excluded participants who completed less than 90 % of the FFQ frequency questions(Reference Lundblad, Andersen and Jacobsen20) and those with implausible daily energy intake (< 500 or > 3500 kcal for women and < 800 or > 4000 kcal for men)(Reference Willett22). Finally, we excluded participants with self-reported diabetes, myocardial infarction, stroke, cancer, coronary surgery/intervention, Crohn’s disease/ulcerous colitis or rheumatoid arthritis (because individuals with these diseases could modify dietary habits) and those aged ≥ 80 years. Thus, the final analytical sample consisted of 1487 participants (55·5 % women) aged 40–79 years.
Fig. 1 Flow chart of the study sample
Dietary assessment
The FFQ(Reference Carlsen, Lillegaard and Karlsen21) in the Norwegian language was handed out in its paper version to all participants. Participants could choose to complete the FFQ at the examination site or return it by mail. Technical assistance to complete the questionnaires was available at the examination site. The FFQ includes 261 questions on the frequency and amount of intake of various food items, dishes, and beverages, as well as meals and dietary supplements(Reference Hopstock, Grimsgaard and Johansen19). Daily energy intake in kilojoules and food and nutrients in grams (g) were calculated using the nutrient calculation system KBS, with database AE14 at the University of Oslo, based on the Norwegian food composition tables from 2014 to 2015. The calculation of daily nutrient intake included the contribution of food items and dietary supplements.
Periodontal assessment and case definition of periodontitis
The oral health examination consisted of a clinical and radiographic examination, performed by calibrated dental hygienists. The clinical examination consisted of probing pocket depth, measured to the closest millimetre with a periodontal probe (UNC15 LM1100-EX) at four sites per tooth, including all natural teeth, except third molars and bleeding on probing. An orthopantomogram was used to assess interdental radiographic marginal bone level (RBL). RBL of interproximal surfaces of all teeth, excluding third molars, was measured linearly with a transparent plastic ruler on the orthopantomogram as described by Holde et al.(Reference Holde, Oscarson and Trovik23) Periodontitis was diagnosed primarily from RBL according to the American Academy of Periodontology and the European Federation of Periodontology classification system of periodontal disease(Reference Caton, Armitage and Berglundh24,Reference Tonetti, Greenwell and Kornman25) . To define periodontitis groups, we used an indirect estimation using RBL as a function of age, i.e. per cent radiographic bone loss divided by the age of the participant (% RBL/age) based on the most severely affected interproximal site in the mouth on participants who had interdental bone loss at ≥ 2 non-adjacent teeth. RBL has been demonstrated as the best predictor of future disease in the absence of treatment, reflects disease history at a given age and includes all risk factors that may have affected bone loss over the individual’s lifetime(Reference Dietrich, Ower and Tank26). For the present study, to achieve a sufficient number of participants in the subgroups, the thresholds of < 0·25, 0·25–0·75 and > 0·75 were applied. Based on these thresholds, three periodontitis groups were created for analysis: (1) no periodontitis or slow bone loss, (2) moderate bone loss and (3) rapid bone loss, respectively.
Number of teeth
Number of teeth was categorised as 25–28, 20–24 and ≤ 19. The threshold of ≤19 teeth was chosen in line with the definition of inadequate dentition proposed by the WHO(27). The threshold of twenty-five teeth represents the average number of teeth among participants in the present study sample.
Covariates
Information on covariates was taken from study questionnaires. Education was categorised as primary (primary/partly secondary: up to 10 years of schooling), secondary (upper secondary: minimum of 3 years) and tertiary (college/university education). Smoking status was categorised as never, former and current smoker. Ever-smoking was estimated by combining former and current smokers. Toothbrushing frequency was categorised as twice/day or more and once/day or less. BMI was calculated as measured weight in kilograms and height in metres squared (kg/m2) and categorised as underweight/normal weight (< 25·0 kg/m2), overweight (25·0–29·9 kg/m2) and obese (≥ 30·0 kg/m2). The few participants with underweight (n 9) were included in the normal weight group. Physical activity in leisure time was assessed by the Saltin-Grimby Physical Activity Level Scale(Reference Grimby, Börjesson and Jonsdottir28) and categorised as sedentary, light and moderate-to-vigorous. Daily energy intake (kilojoules/d) was divided into tertiles.
Identification of dietary patterns
Intake of 235 foods and beverages (g/d) was used in the analysis. According to similarities in nutritional composition and usage, single food and beverage intakes were manually aggregated into forty-nine groups (see online supplementary material, Supplemental Table 1). The forty-nine groups were used in a principal component analysis (PCA) with a correlation matrix to identify linear combinations of food groups that explained the greatest variance. Statistical software performs standardisation by default using correlation matrix. The resulting components were rotated orthogonally for interpretability. The number of principal components retained was based on eigenvalues > 1·0, inspection of the scree plot (Fig. 2) and interpretability. Loadings of food category variables > |0·20| were used to characterise principal components as dietary patterns. The Kaiser-Meyer-Olkin measure of sampling adequacy was 0·76, and the Bartlett test of sphericity P < 0·001 was satisfactory. When PCA was stratified by sex and age (40–49 v. 50–79 years), relatively similar dietary patterns were identified (data not shown). Accordingly, PCA and all subsequent statistical analyses were performed on the entire sample. When we compared participants with valid dietary data, no major chronic diseases, with and without oral examination (n 8360) to those in the present study sample (n 1487), the same dietary patterns were identified. There were only minor differences in the magnitude of factor loadings and in variability, which was explained by certain principal components. High correlation coefficients between component scores of identical patterns were detected (data not shown). We compared PCA analyses, based on unadjusted and energy-adjusted weights. We chose gram weights as input variables in PCA analysis, as unadjusted patterns were more interpretable. Five energy-adjusted dietary patterns were extracted. Four energy-adjusted patterns had similar loadings and described similar dietary patterns when compared with the unadjusted patterns. However, the fifth energy-adjusted dietary pattern was difficult to interpret. Therefore, we performed energy adjustment later in the analytical process by including energy intake in regression models. A detailed description of energy-adjusted dietary patterns and regression models with them can be found in online supplementary material, Supplemental Appendix 2: Energy-adjusted dietary pattern analysis.
Fig. 2 Scree plot for the identification of dietary patterns (components) by principal component analysis. Food intakes (g/d) were aggregated into 49 food groups and used as input variables
Statistical analysis
Component dietary pattern scores were split into tertiles (tertile 1 = low intake, tertile 2 = moderate intake, tertile 3 = high intake). We calculated descriptive statistics for the full sample and for those in tertiles 1 and 3 of the identified dietary patterns. We used multinomial logistic regression models to study the association between tertiles of dietary patterns and periodontitis groups and number of teeth adjusted for sex, age, education, smoking status, toothbrushing frequency, BMI, physical activity and energy intake. We presented results as OR with 95 % CI. To test for linear trend across tertiles of dietary patterns, we used the median of each tertile and treated it as a continuous variable. To test the significance of the interaction, we included a product term with the respective stratification variable, i.e. sex, age group (40–49 years v. 50–79 years), smoking (never- v. ever-smoker), and BMI (< 30·0 kg/m2 v. ≥ 30·0 kg/m2) and the median value of the dietary pattern’s tertiles to test the significance of the interaction. No evidence supporting an interaction effect (P < 0·05) was found; thus, these results were not presented.
All analyses were conducted using STATA version 16 (StataCorp, College Station, Texas, USA). All tests were two-tailed, and P-values < 0·05 were considered to be statistically significant.
Results
Characteristics of the study sample
The study sample comprised 48·6 % participants with moderate bone loss and 6·1 % with rapid bone loss. In total, 8·1 % of participants had 4–19 teeth (Table 1).
Table 1 Characteristics of the study population
Values are numbers (percentages) for categorical variables and mean (sd) for continuous variables.
* Low (primary/partly secondary: up to 10 years of schooling), medium (upper secondary: minimum of 3 years) and high (college/university education).
Characteristics of dietary patterns
Figure 2 shows the scree plot for identification of dietary patterns. Four dietary patterns that explained 24·3 % of the total variability in food intake were identified and labelled ‘Fruit and vegetables’, ‘Westernised’, ‘Meat/fish and potatoes’ and ‘Refined grain and dessert’ (Table 2) based on the highest food group loadings. For the fruit and vegetables pattern, the following food groups loaded ≥|0·20|: vegetables, fruit, berries, dried fruit, nuts or peanut butter, fatty fish, seafood, vegetarian dish, beans/lentils and stew/soup with fish. The Westernised pattern was characterised by high intake of processed meat dishes or fast food, rice or pasta, salty snacks, chicken, fried potato dishes, tomato sauces, salad dressing and wok with meat/chicken. The meat/fish and potatoes pattern loaded positively for red meat, sausage or bacon, stew with meat or chicken, lean fish, processed fish, boiled/baked or mashed potatoes, and sauce butter/margarine melted or creamy dressing. The refined grain and dessert pattern loaded positively for food high in refined grains, sweet spreads or sweeteners, food containing 50–100 % whole grains, cakes or dessert, butter, margarine or mix of butter, margarine and oil as a spread, whey cheese and negatively for wine.
Table 2 Loading matrix (≥ |0·20|) and explained variances for the first four PCs identified by PCA
PC: Principal Components; PCA: Principal Component Analysis.
Characteristics of the study population according to tertiles of dietary pattern are shown in Table 3. Participants in tertile 3 of the fruit and vegetables pattern were more likely to be women, have tertiary education, be never-smokers and be more physically active. Participants in tertile 3 of the Westernised pattern were more likely to be men, younger and have tertiary education. Participants in tertile 3 of the meat/fish and potatoes pattern were more likely to be men, older, have primary or secondary education and be ever-smokers. Participants in tertile 3 of the refined grain and dessert pattern were more likely to be men and never-smokers.
Table 3 Characteristics of the study population according to tertiles of dietary pattern scores
Values are percentages for categorical variables and mean (sd) for continuous variables.
* Low (primary/partly secondary: up to 10 years of schooling), medium (upper secondary: minimum of 3 years) and high (college/university education).
Association between dietary patterns and periodontitis
Compared to tertile 1 of the fruit and vegetables pattern, those in tertile 3 had lower odds of periodontitis characterised by rapid bone loss (OR 0·49, 95 % CI 0·25, 0·98, P = 0·043; P trend = 0·050) after adjustment for confounders (Table 4).
Table 4 Regression models between tertiles of dietary pattern scores and periodontitis groups
All models adjusted for sex, age, education, smoking status, toothbrushing frequency, BMI, physical activity and energy intake.
* To test for linear trend across tertiles of the dietary pattern score, we used the median of each tertile and treated the variable as continuous variable.
Association between dietary patterns and number of teeth
Participants with ≤ 19 teeth were more likely to have a diet characterised by higher intake of foods included in the refined grain and dessert pattern (Table 5). Participants in tertile 2 had 2·10-fold increased odds of having ≤ 19 teeth than 25–28 teeth. Participants in tertile 3 of the refined grain and dessert pattern had 2·38- and 3·52-fold increased odds of having ≤ 19 teeth than 20–24 and 25–28 teeth, respectively (Table 5). Foods correlated with the Westernised pattern were consumed less often by participants with ≤ 24 teeth (Table 5).
Table 5 Regression models between tertiles of dietary pattern scores and number of teeth categories
All models adjusted for sex, age, education, smoking status, toothbrushing frequency, BMI, physical activity and energy intake.
* To test for linear trend across tertiles of the dietary pattern score, we used the median of each tertile and treated the variable as continuous variable.
† For model with tertiles of Westernised dietary pattern score 20–24 teeth and ≤ 19 teeth categories were combined, and a dichotomous variable was used (≤ 24 teeth v. 25–28 teeth) as there were few participants (n 5 (1·0 %)) who had ≤ 19 teeth and were categorised into third tertile of the Westernised dietary pattern.
Discussion
We aimed to explore the association between dietary patterns and (1) periodontitis and (2) number of teeth, in a population-based sample from Norway. Out of four identified dietary patterns, the fruit and vegetables pattern was associated with 50 % lower odds of the periodontitis characterised by rapid bone loss. The refined grain and dessert pattern was associated with having ≤ 19 teeth.
Diet is a complex exposure; combinations of food groups or nutrients may have different effects when compared to single food and nutrient exposures. A study by Blostein et al.(Reference Blostein, Jansen and Jones29) demonstrated that, when food groups that had the highest loadings for dietary patterns associated with caries were tested as predictors in diet-caries associations, no significant relations were found; however, dietary patterns were found to be associated with caries. Our findings are consistent with the study by Wright et al.(Reference Wright, McKenna and Nugent8), which used a data-driven approach to derive dietary patterns using food groups. Their study found associations between a dietary pattern rich in salad, fruit, vegetables, poultry, seafood, water and tea and a lower extent of clinical attachment loss. In the present study, the fruit and vegetables pattern was associated with periodontitis and included similar food groups. A more recent cross-sectional study reported that the ‘high micronutrient (i.e. β-carotene, vitamin B6, folate, vitamin C, vitamin E, iron, potassium and magnesium) and fibre’ nutrient pattern assessed by the posteriori approach using nutrient intakes was associated with reduced risk of self-reported periodontal disease(Reference Watson, Woodside and Winning30). We can expect that the intake of these nutrients is higher for those in tertile 3 of the fruit and vegetables pattern. Moreover, fruits and vegetables contain high levels of phytochemicals, bioactive components that may contribute to the beneficial effects of healthy diets. The Western or Prudent dietary patterns are often derived from exploratory patterns, and these patterns were also identified in the present study(Reference Schulz, Oluwagbemigun and Nöthlings31). However, exploratory patterns identified in different groups and populations can vary and have different levels of reproducibility, making comparison of studies difficult. Nevertheless, our findings were in line with previous studies and complemented those of hypothesis-driven methods.
It has been suggested that diet may affect periodontitis by shaping the microbiota and modulating systemic low-grade inflammation(Reference Martinon, Fraticelli and Giboreau4,Reference Minihane, Vinoy and Russell32) . Further, nutrients are involved in the formation of bones and teeth, and nutrients act as antioxidants, methyl donors and cofactors that can affect DNA methylation and contribute to the reduction of DNA damage(Reference Beetch, Harandi-Zadeh and Shen33). Many vitamins and trace elements, such as vitamins A, D, C, E, B6, and B12, folate, zinc, iron, copper and selenium, play an important role in the immune response to infection(Reference Gombart, Pierre and Maggini34). In addition to micronutrient deficiencies(Reference Varela-López, Giampieri and Bullón35), macronutrient imbalance, for example an increased consumption of refined carbohydrates and low fibre intake, may be involved in the pathogenesis of periodontitis(Reference Hujoel36,Reference Woelber, Gärtner and Breuninger37) .
Recent studies have indicated that high intake of fermentable carbohydrates (mainly sucrose) not only significantly increases the risk of caries but is also associated with the risk of periodontal disease(Reference Nyvad and Takahashi38). No association could be confirmed between the refined grain and dessert pattern and periodontitis in the present study. However, the refined grain and dessert dietary pattern was associated with inadequate dentition. Dental caries and periodontitis are the major causes of tooth loss, but the mechanisms by which excessive intake of carbohydrates relates to caries and periodontitis are supposed to be different. The development of caries requires sugars and acidogenic, acid-tolerant bacteria(Reference Pitts, Zero and Marsh39). Demineralisation of the enamel occurs when plaque bacteria metabolise dietary sugars and produce organic acids, which increase the solubility of the calcium hydroxyapatite that is present in the hard tissue of teeth. When it comes to periodontitis, sugars may confer pro-inflammatory properties to microbiota in the mouth and gut, thus contributing to local and systemic inflammation(Reference Martinon, Fraticelli and Giboreau4,Reference Santonocito, Giudice and Polizzi40) . It has been suggested that the dysbiosis of the oral microbiota may trigger changes in the gut microbiota, which creates a higher predisposition for the development of various chronic diseases. We have previously shown that periodontitis was associated with cardiovascular risk and higher C-reactive protein concentrations(Reference Petrenya, Hopstock and Holde41). Owing to the relation between diet and chronic inflammation, several studies reported associations between Dietary Inflammatory Index and periodontitis(Reference Li, Chen and Schuller6,Reference Machado, Botelho and Viana7,Reference Lieske, Moszka and Borof11) and more lost teeth(Reference Kotsakis, Chrepa and Shivappa17).
The present study is cross-sectional; therefore, we cannot draw conclusions about causal relationships between diet and oral health outcomes. Reverse causation can occur when people change their diet due to inadequate dentition or oral health disease. In the present study, 8·1 % of individuals had ≤ 19 teeth. Previous studies have shown that severe tooth loss and masticatory impairment may result in dietary changes like limited consumption of fruits and vegetables, and increased consumption of sugary and easy-to-chew foods(Reference Ervin and Dye42,Reference Kossioni43) . We tried to account for this challenge by performing regression analysis on the associations between the fruit and vegetables pattern and periodontitis after the exclusion of individuals with ≤ 19 teeth, and similar results were observed (data not shown). Moreover, some adults with periodontitis might change their dietary habits due to pain, discomfort, high dentinal hypersensitivity(Reference Idon, Esan and Bamise44), impaired senses of smell and taste,(Reference Schertel Cassiano, Leite and López45) or other reasons. One study reported that the periodontitis group avoided alcohol, sweets, carbonated beverages, hot and cold drinks, cold food, and hard textured and fibrous foods more often than controls(Reference Almoznino, Gal and Levin46). We also found that the Westernised dietary pattern was less common among participants with ≤ 24 teeth. We observed that the group with ≤ 24 teeth included a higher percentage of older than younger participants (40–49 years: n 67, 14·7 %; 50–59 years: n 95, 19·4 %; 60–69 years: n 188, 46 %; 70–79 years: n 440, 67·7 %; data not shown).
Previous studies found a modifying effect of obesity and smoking on the association between poor diet and periodontitis(Reference Alhassani, Hu and Li5,Reference Alhassani, Hu and Rosner10,Reference Jauhiainen, Ylöstalo and Knuuttila47) . We found no evidence of an interaction effect of obesity and smoking on the association between dietary patterns and periodontitis (data not shown).
Definition of periodontitis was based on the 2017 World Workshop on the Classification of Periodontal and Peri-Implant Diseases and Conditions, which is the most recent classification. Tonetti & Claffey(Reference Tonetti and Claffey48) suggested that the periodontitis progression case definition that demonstrates longitudinal attachment loss should be considered in risk factor research. By using the rate of bone loss related to age rather than stages, we were able to consider disease susceptibility due to life-long exposure to different causal factors, including established, modifiable risk factors like smoking and dysglycaemia,(Reference Dietrich, Ower and Tank26), and to achieve smaller age differences between the periodontitis groups. We used grading to identify periodontitis cases. Due to the few participants with grade C, and to get a more statistically robust group with advanced periodontitis, we slightly modified the cut-off and labelled the different levels of bone loss as slow, moderate, and rapid(Reference Dietrich, Ower and Tank26).
Strengths
The present study has several strengths. The Tromsø Study is a well-designed population-based cohort where data collection was performed by trained personnel using standardised protocols and instruments. To our knowledge, this is the first study that relates a posteriori food group-based dietary patterns and two objectively measured oral health outcomes (periodontitis and number of teeth). Assessments of dietary intake were based on a previously validated FFQ that captures the habitual dietary intake, and strict criteria were applied to exclude participants with unreliable dietary data. In addition, participants with major chronic diseases, including diabetes, (which might lead to changes in dietary habits) were excluded from the analysis. These exclusions were made to improve the internal validity of the present study; however, exclusions may also have resulted in less generalisable results. One study demonstrated the effect of interactions between diabetes and dietary patterns on periodontitis(Reference Feng, Jin and Dong49). Participants who consumed an anti-inflammatory diet and did not have diabetes experienced the lowest risks of periodontitis and tooth loss. However, in the context of diabetes, the efficacy of such a diet may be weakened or even eliminated. The main results based on unadjusted patterns were comparable with energy-adjusted patterns (see online supplementary material, Supplemental Appendix).
Limitations
The main limitation is the cross-sectional design of the present study, which left us unable to determine the direction of the relationships between dietary patterns and outcomes. Bias due to self-reporting and selection bias may also have occurred. The present study is observational in nature; therefore, the observed associations might be explained by unmeasured or residual confounding. PCA is the most commonly used data-driven reduction technique to identify dietary patterns. However, the limitations of PCA include subjectivity related to the selection of food groups and the determination of retained components, limited reproducibility in different populations, correlations/interactions of components with many lifestyle characteristics, and that the retained patterns can only explain part of the total variation in food intake, i.e. 24 % in our study(Reference Zhao, Li and Gao15). There are some indications of an interaction between genetic risks for age-related diseases and dietary patterns(Reference Samuelsson, Najar and Wallengren50); however, it is not known if this is the case for oral diseases.
Conclusion
Our study contributes to the evidence that overall diet may be associated with advanced periodontitis and tooth loss. Oral hygiene routines, periodontal treatment, and smoking cessation are recommended for patients with periodontitis, but dietary recommendations have not yet been developed due to limited evidence on the causal relationship. More likely, associations between dietary patterns and periodontitis are bidirectional and can be impacted by additional risk factors. Our findings are in line with current official chronic disease prevention dietary guidelines that encourage eating less foods rich in refined grains, sugar and saturated fats – especially processed foods – and eating more vegetables, fruits, berries, nuts, fish and legumes. To what degree diet is an essential component in the prevention of chronic inflammatory diseases remains uncertain; further prospective studies that measure the effectiveness of dietary interventions on periodontitis are necessary.
Acknowledgments
The authors are grateful to all the participants and staff of the seventh survey of the Tromsø Study.
Financial Support
The project was financed by the authors’ institutions, the Tromsø County Council and the Norwegian Directorate of Health.
Conflict of interest
All authors declare no conflict of interest.
Authorship
B.J. designed and planned the data collection regarding oral health in the Tromsø study. B.J. and L.A.H. contributed to the data collection. N.P. contributed to the conception and design of the study, performed the statistical analyses, drafted and revised the manuscript. M.B., L.A.H., G.E.H. and B.J. contributed to the conception and design of the study, the interpretation and discussion of findings, and reviewed and approved the final manuscript. G.E.H. was responsible for the creation of periodontal variables and for the definition of periodontitis together with B.J. All authors have read and approved the final version of the manuscript. The authors declare that they have no known competing financial interests or personal relationships that could appear to influence the work reported in this paper.
Supplementary material
For supplementary material accompanying this paper visit https://doi.org/10.1017/S1368980023002690
Ethics of human subject participation
This study was conducted according to the guidelines laid down in the Declaration of Helsinki, and all procedures involving research study participants were approved by the Regional Committees for Medical and Health Research Ethics North (REC-North 05·11·2019 ref. 406077). Written informed consent was obtained from all participants.
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