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Spin-labelled nucleic acids

Published online by Cambridge University Press:  17 March 2009

S. G. Kamzolova  and
G. B. Postnikova
S. G. Kamzolova
Affiliation:
Institute of Biological Physics, Pushchino, Moscow Region, USSR
G. B. Postnikova
Affiliation:
Institute of Biological Physics, Pushchino, Moscow Region, USSR
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Extract

The spin label method developed by McConnell 15 years ago is now widely used in studies of the structure and dynamic properties of a variety of the biological systems such as proteins and protein complexes, lipids and membranes, nucleic acids, nucleoproteins, etc.

The ESR spectrum of the nitroxide radcal – the spin label – is very sensitive to its microenvironment and permits easy registration of even subtle alterations in it. If spin labels are attached to different sites of a macromolecule the information can be gained about conformational properties of all these local regions and, as a result, about the dynamic behaviour of the object as a whole.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 14 , Issue 2 , May 1981 , pp. 223 - 288
Copyright
Copyright © Cambridge University Press 1981

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References

REFERENCES

Adler, V. V., Poverenny, A. M., Podgorodnichenko, V. D. & Shapot, V. S. (1973). Study on the process of transcription using antibodies to DNA. Mol. Biol. (USSR) 7, 203–211.Google Scholar
Arnott, S. (1970). The geometry of nucleic acids. Prog. Biophys. & molec. Biol. 21, 265–319.CrossRefGoogle ScholarPubMed
Arnott, S. (1971). The structure of transfer RNA. Prog. Biophys. & molec. Biol. 22, 179–213.CrossRefGoogle Scholar
Artyukh, R. I., Postnikova, G. B., Sukhorukov, B. I. & Kamzolova, S. G. (1972). Study of spin-labelled DNA. Chemical modification of DNA with stable free radical 2,2,6,6-tetramethyl-4-bromoacetoxypiperidine- 1-oxyl. Biokhimiya (USSR) 32, 902–906.Google Scholar
Baev, A. S., Lyubchenko, Yu. P., Lazurkin, Yu. S., Trifonov, E. M. & Frank-kamenetsii, M. D. (1972). Study of easily melting T2 DNA regions by means of electronic microscopy and kinetic formaldehyde method. Mol. Biol. (USSR) 6, 760–766.Google Scholar
Beabealashvilli, R. Sh., Ivanov, V. I., Minchenkova, L. E. & Savochkina, L. P. (1972). RNA polymerase-DNA complex. I. The study of conformation of nucleic acids at the growing point of RNA in an RNA polymerase-DNA system. Biochim. biophys. Acta 259, 35–43.CrossRefGoogle Scholar
Beres, L. & Lucas-Lenard, J. (1973). Studies of the fluorescence of the Y base of yeast tRNAphe effect of pH, aminoacylation, and interaction with elongation factor Tu. Biochemistry, N.Y. 12, 3998–4002.CrossRefGoogle Scholar
Bobst, A. M. (1972). Studies on spin-labelled polyriboadenylic acid. Biopolymers 11, 1421–1433.CrossRefGoogle Scholar
Bobst, A. M. (1979). Applications of spin labelling to nucleic acids. In Spin Labelling. Vol. II. Theory and Applications (Berliner, L. J.), pp. 291–345. New York: Academic Press.Google Scholar
Bobst, A. M., Hakam, A., Langemeier, P. W. & Kouldo, S. (1979). Electron spin resonance melting of chemically spin-labelled nucleic acids. Archs Biochem. Biophys. 194, 171–178.CrossRefGoogle Scholar
Botchan, P. (1976). An electron microscopic comparison of transcription on linear and superhelical DNA. J. molec. Biol. 105, 161–177.CrossRefGoogle ScholarPubMed
Brams, J. & Mammaerts, W. F. H. (1964). A study of conformational of nucleic acids in solution by means of circular dichroism. J. molec. Biol. 10, 73–79.CrossRefGoogle Scholar
Brandts, J. F., Jackson, W. M. & Yao-Chung, Ting. T. (1974) A calorimetric study of the thermal transitions of three specific tRNAs. Biochemistry, N.Y. 13, 3595–3600.CrossRefGoogle Scholar
Burd, J. F., Wartell, R. M., Dodgson, J. B. & Wells, R. D. (1975), Transmission of stability (telestability) in DNA. J. biol. Chem. 250, 5109–5113.CrossRefGoogle Scholar
Burd, J. F., Larson, J. E. & Wells, R. D. Further studies on telestability in DNA: the synthesis and characterization of the duplex block polymers d(C20A10). d(T10G20) and d(C20A15). d(T15G20). J. biol. Chem. 250, 6002–6007.CrossRefGoogle Scholar
Calendar, R. & Berg, P. (1967). D-tyrosyl RNA: formation, hydrolysis and utilization for protein synthesis. J. molec. Biol. 26, 39–54.CrossRefGoogle ScholarPubMed
Caron, M., Brusson, N. & Dugas, H. (1976). Evidence for a conformational change in tRNAphe upon aminoacylation. J. biol. Chem. 251, 1529–1530.CrossRefGoogle ScholarPubMed
Caron, M. & Dugas, H. (1976 a). Specific spin-labelling of transfer ribonucleic acid molecules. Nucl. Acids Res. 3, 19–34.CrossRefGoogle Scholar
Caron, M. & Dugas, H. (1976 b). A spin label study of the thermal unfolding of secondary and tertiary structure in E. coli transfer RNAs. Nucl. Acids Res. 3, 35–47.CrossRefGoogle Scholar
Caspary, W. J., Greene, J. J., Stempel, L. M. & Ts'o, P. O. P. (1976). Spin labelled nucleic acids. Nucl. Acids Res. 3, 847–863.CrossRefGoogle Scholar
Chamberlin, M. (1974). The selectivity of transcription. A. Rev. Biochem. 43, 721–741.CrossRefGoogle ScholarPubMed
Cherny, D. J., Alexandrov, A. A., Zarudnaya, M. I., Kosaganov, Yu. N., Lazurkin, Yu. S., Beabealashvilli, R. Sh. & Savochkina, G. P. (1977). Investigation of the binding of E.coli RNA polymerase to DNA from bacteriophages T2 and T7 by kinetic formaldehyde method and electron microscopy. Eur. J. Biochem. 79, 309–3 17.CrossRefGoogle ScholarPubMed
Crothers, D. M., Cole, P. E., Hilbers, C. W. & Schulman, R. G. (1974). The molecular mechanism of thermal infolding of E. coli formylmethionine tRNA. J. molec. Biol. 87, 63–88.CrossRefGoogle Scholar
Danchin, A. (1972). tRNA structure and binding sites of cations. Biopolymers 11, 1317–1333.CrossRefGoogle ScholarPubMed
Dausse, J., Sentenac, A. & Fromageot, P. (1976). Interaction of RNA polymerase from E. coli with DNA. Effect of temperature and ionic strength on selection of T7 DNA early promoters. Eur. J. Biochem. 63, 387–393.CrossRefGoogle Scholar
Davanloo, P., Sprinzl, M. & Cramer, F. (1979). Proton nuclear magnetic resonance of minor nucleosides in yeast tRNAphe. Conformational changes as a consequence of aminoacylation, removal of the Y base, and codon-anticodon interaction. Biochemistry, N.Y. 18, 3189–3199.CrossRefGoogle Scholar
Dickson, R. C., Abelson, J., Barnes, W. M. & Resnikoff, W. S. (1975). Genetic regulation. The lac control region. Science, N.Y. 187, 27–35.CrossRefGoogle ScholarPubMed
Dugas, H. (1977). Spin-labelled nucleic acids. Acc. Chem. Res. 10, 47–54.CrossRefGoogle Scholar
Eigen, M. (1968). The dynamic of conformational changes in helical macromolecules. J.chem. Phys. 65, 53–60.Google Scholar
Eisenberg, H. & Cohen, G. (1968). An interpretation of the low-angle X-ray scattering of DNA solutions. J. molec. Biol. 37, 355–362.CrossRefGoogle ScholarPubMed
Freed, J. H. & Fraenkel, G. K. (1963). Intramolecular hydrogen bonding in electron spin resonance spectra. J. chem. Phys. 39, 326–330.CrossRefGoogle Scholar
Fresco, J. R., Adams, A., Ascione, R., Henley, D. & Lindahl, T. (1966). Tertiary structure in transfer ribonucleic acids. Cold Spring Harb. Symp. Quant. Biol. 31, 527–538.CrossRefGoogle ScholarPubMed
Girshivich, A. S., Grachev, M. A., Knorre, D. G., Kumarev, V. P. & Levintahl, V. I. (1971). Reaction of a spin-labelled carbodiimide with nucleosides, poly U and tRNA. FEBS Lett. 14, 199–202.CrossRefGoogle Scholar
Grishovich, A. S., Grachev, M. A. & Obukhova, L. V. (1968). Chemical modification of tRNAphe by N-cyclohexyl, N′-(4-methylmorpholinyl)-ethyl carbodiimide. Molecular Biology (USSR) 8, 351–363.Google Scholar
Gralla, J. & Crothers, D. M. (1973). Free energy of imperfect nucleic acid helixes. II. Small hairpin loops. J. molec. Biol. 72, 497–511.CrossRefGoogle Scholar
Griffith, O. H., Cornell, D. W. & McConnell, H. M. (1965). Nitrogen hyperfine tensor and g-tensor of nitroxide radicals. J. chem. Phys. 43, 2909–2910.CrossRefGoogle Scholar
Griffith, O. H. & McConnell, H. M. (1966). A nitroxide-maleimide spin-label. Proc. natn. Acad. Sci. U.S.A. 55, 8–19.CrossRefGoogle ScholarPubMed
Hamilton, C. L. & McConnell, H. M. (1968). Spin labels. In Structural Chemistry and Molecular Biology (ed. Rich, A. and Davidson, N.), pp. 115–123. San Francisco. W. H. Freeman.Google Scholar
Hänggi, U. J. & Zachau, H. G. (1971). Partial nuclease digestion of tRNAs and aminoacylated tRNAs. Eur. J. Biochem. 18, 496–502.CrossRefGoogle Scholar
Hara, H., Horiuchi, T., Saneyoshi, M. & Nishimura, S. (1970). 4-Thiouri-dine-specific spin labeling of E. coli transfer RNA. Biochem. biophys. Res. Commun. 38, 305–311.CrossRefGoogle ScholarPubMed
Hoffman, B. M., Schofield, P. & Rich, A. (1969). Spin-labelled transfer RNA. Proc. natn. Acad. Sci. U.S.A., 62, 1195–1202.CrossRefGoogle Scholar
Holley, R. W., Apgar, J., Everett, G. A., Madison, J. T., Marguisse, M., Merrill, S. H., Penwich, J. R. & Lamir, A. (1965). Structure of a ribonucleic acid. Science, N.Y. 147, 1462–1466.CrossRefGoogle ScholarPubMed
Inman, R. B. (1967). Denaturation maps of the left and right sides of the lambda DNA molecule determined by electron microscopy. J. molec. Biol. 28, 103–116.CrossRefGoogle ScholarPubMed
Ivanov, V. I., Minchenkova, L. E., Minjat, E. E., Frank-Kamenetskii, M. D. & Schyolkina, A. K. (1974). The B to A transition of DNA in solution. J. molec. Biol. 87, 817–835.CrossRefGoogle Scholar
Ivanov, V. I., Minchenkova, L. E., Schyolkina, A. K. & Poletayev, A. J. (1973). Different conformations of double-stranded nucleic acid in solution as revealed by circular dichroism. Biopolymers 12, 89–110.CrossRefGoogle ScholarPubMed
Jack, A., Lander, J. E., Rhodes, D., Brown, R. S. & Klug, A. (1977). A crystallographic study of metalbinding to yeast phenylalanin tRNA. J. molec. Biol. 111, 315–328.CrossRefGoogle Scholar
Kabat, D., Hoffman, B. & Rich, A. (1970). Synthesis and characterization of a spin-labelled aminoacyl transfer ribonucleic acid. Biopolymers 9, 95–101.CrossRefGoogle Scholar
Kamzolova, S. G., Artyukh, R. I. & Elfimova, L. I. (1977). Studies on template properties of T2-DNA modified with 2,2,6,6 tetramethyl-4- bromoacetoxypiperidine-toxyl in RNA. polymerase system. Biokhimiya (USSR) 42, 1117–1122.Google Scholar
Kamzolova, S. G., Kolontarov, A. I., Elfimova, L. I. & Sukhorukov, B. I. (1973). The study of conformational changes of binary complex of RNA polymerase from E. coli with T2-DNA. Dokl. Akad. Nauk USSR 208, 245–247.Google Scholar
Kim, S. H., Quigley, G. J., Suddath, F. L., McPherson, A., Sneden, D., Kim, J. J., Weinzierl, J. & Rich, A. (1973). Three-dimensional structure of yeast tRNAphe: folding of the polynucleotide chain. Science, N. Y. 179, 285–288.CrossRefGoogle Scholar
Kivelson, D. (1960). Theory of ESR linewidths of free radicals. J. chem. Phys. 33, 1094–1098.CrossRefGoogle Scholar
Kosaganov, Yu. N., Zarudnaya, M. I., Beabealashvilli, R. Sh., Lazurkin, Yu. S., Frank-Kamenetsii, M. D. & Savochkina, L. D. (1971). Local unwinding of DNA during RNA synthesis in vitro. Nature (New Biol.) 231, 212–215.Google ScholarPubMed
Kraevskii, A. A., Purigyin, P. P., Rudzite, L. N., Belova, Z. S. & Gottikh, B. P. (1968). Aminoacyl derivatives of nucleosides, nucleotides and polynucleotides. I. The synthesis of 3′(2′)-o-aminoacylnucleotides. Izvestia Akad. Nauk USSR 2, 378–382.Google Scholar
Kruglakova, K. E. & Zhiltsova, V. A. (1970). Intermolecular interaction in DNA solutions under the action of UV-irradiation. Stud. Biophysics 24/25, 429–431.Google Scholar
Kumarev, V. P. & Knorre, D. G. (1970). Water soluble spin-labelled carbodiimide. Dokl. Akad. Nauk USSR, 193, 103–105.Google Scholar
Kuzetzov, A. N., Wasserman, A. M., Volkov, A. I. & Korst, N. N. (1971). Determination of rotational correlation time of nitric oxide radicals in a viscous medium. Chem. Phys. Letters 12, 103–107.Google Scholar
Lawley, P. D. & Brookes, P. (1967). Interstrand cross-linking of DNA by difunctional alkylating agents. J. moiec. Biol. 25, 143–160.CrossRefGoogle ScholarPubMed
Levy, J. & Biltonen, R. (1972). Thermodynamic studies of tRNAs. III. Thermodynamic model for the thermal unfolding of yeast tRNAphe Biochemistry, N.Y. 11, 4145–4152.CrossRefGoogle Scholar
Lichtenstein, G. I. (1974). Spin-labelling Technique in Molecular Biology. Moscow: Nauka.Google Scholar
Luzzati, V., Nikolaieff, A. & Masson, F. (1961). Structure de l'acide désoxyribonucleique en solution, étude par diffision des rayons X aux petits angles. J. molec. Biol. 3, 185–201.CrossRefGoogle Scholar
McConnell, H. M. & McFarland, B. G. (1970). Physics and chemistry of spin labels. Q. Rev. Biophys. 3, 91–136.CrossRefGoogle ScholarPubMed
McIntosh, A. R., Caron, M. & Dugas, H. (1973). A specific spin labelling of the anticodon of E. coli tRNAGlu. Biochem. biophys. Res. Commun. 55, 1356–1363.CrossRefGoogle Scholar
Mangel, W. & Chamberlin, M. (1974). Studies of ribonucleic acid chain initiation by E. coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. II. The effect of alteration in ionic strength on chain initiation and on the conformation of binary complexes. J. biol. Chem. 249, 3002–3009.CrossRefGoogle Scholar
Melnikova, A. F., Beabealashvilli, R. Sh. & Mirzabekov, A. D. (1978). A study of unwinding of DNA and shielding of the DNA grooves by RNA polymerase using methylation with dimethylsulphate. Eur. J. Biochem. 84, 301–311.CrossRefGoogle ScholarPubMed
Mil', E. M., Bobovich, S.I., Azizov, Yu. M., Krinitskaya, L.A., Zhiltsova, V. M., Zhizhina, G. P. & Kruglaykova, K. E. (1975). Conformational transitions in DNA from normal and cancer cells. Biofisika (USSR) 20, 757–761.Google Scholar
Mil', M., Zavriev, S. K., Grigoryan, L. L. & Kruglaykova, K. E. (1973). Studies of conformational changes of spin labelled DNA. Dokl. Akad. Nauk USSR 209, 217–220.Google Scholar
Mil', E. M., Zhiltsova, V. M. & Kruglyakova, K. E. (1974). The application of the method of paramagnetic label for studying conformational changes in DNA under UV-irradiation. Biofizika (USSR), 19, 601–604.Google Scholar
Nikiforov, V. G. & Astaurova, O. B. (1971). Template synthesis of ribonucleic acids by RNA polymerase. In Molecular Basis of Protein Biosynthesis. pp. 21–121. Moscow: Nauka.Google Scholar
Nikiforov, V. G. & Sograf, Yu. N. (1977). The regulation of gene activity of bacteria. VINITI Ser. Molek. Biologia, 13 (ed. Khesin, R. B.) Moscow.Google Scholar
Petrov, A. I., Postnikova, G. B. & Sukhorukov, B. I. (1972). The application of 2,2,5, 5-tetramethyl-3-carbonylpyrroline-1-oxyl) imidazole for preparation of spin labelled nucleic acids. Izvestia Akad. Nauk USSR 6, 1453–1454.Google Scholar
Postnikova, G. B., Artyukh, R. I., Petrov, A. I. & Sukurukov, B. I. (1972). Study of DNA by the spin label method. Proc. IVth Int. Biophys. Congr. b. 1, pp. 92–94.Google Scholar
Printz, M. P. & von, Hippel P. H. (1968). On kinetic of hydrogen exchange in DNA. Biochemistry, N.Y. 7, 3194–3206.CrossRefGoogle Scholar
Privalov, P. L., Kafiani, K. A., Monacelidze, D. R., Mrevlishvili, G. M. & Magaladze, V. A. (1966). DNA denaturation and the state of its water. In Nucleic Acids (Proc. IInd Conf. Nucleic Acids), pp. 15–19. Moscow: Medizina.Google Scholar
Quigley, G. J. & Rich, A. (1976). Structural domains of transfer RNA molecules. Science, N. Y. 194, 796–806.CrossRefGoogle ScholarPubMed
Remold-O'Donnel, E. & Zillig, W. (1969). Purification and properties of DNA-dependent RNA polymerase from Bacillus stearothermophilius. Eur. J. Biochem. 7, 318–323.CrossRefGoogle Scholar
Rich, A. (1978). Transfer RNA: three-dimensional structure and biological function. Trends in biochem. Sci. 3, 34–37.CrossRefGoogle Scholar
Rich, A. & Schimmel, P. R. (1977). Structure organization of complexes of transfer RNAs, with aminoacyl-tRNA-synthetases. Nucl. Acids. Res. 4, 1649–1666.CrossRefGoogle Scholar
Riesner, D., Maas, G., Thiobe, R., Philipsen, P. & Zachau, H. G. (1973). The conformational transitions in yeast tRNAphe as studied with tRNAPhe fragments. Eur. J. Biochem. 36, 76–88.CrossRefGoogle ScholarPubMed
Robertus, J. D., Ladnev, J. E., Finch, J. T., Rhodes, D., Brown, R. S., Clark, B. F. C. & Klug, A. (1974). Structure of yeast phenylalanine tRNA at 3 Å resolution. Nature, Lond. 250, 546–551.CrossRefGoogle ScholarPubMed
Rodrigufz, A., Tougas, G., Brisson, N. & Dugas, H. (1980). Interaction of 70 S Ribosomes from E. coli with spin-labelled N-Cbz-Phe-tRNAPhe. J. Biol. Chem. 255, 8116–8120.CrossRefGoogle Scholar
Römer, R., Riesner, D., Coutts, S. M. & Maas, G. (1970). The coupling of conformational transition in alanine specific tRNA from yeast studied by a modified differential absorption technique. Eur. J. Biochem. 15, 77–84.CrossRefGoogle Scholar
Savochkina, L. P., Beabealashvilli, R. Ch., Gurskii, J. V., Zasedatelev, A. S., Ivanov, V. I. & Minchenkova, L. E. (1973). The study of physical structure of DNA. sites binding RNA polymerase. In Conformational Changes of Biopolymers in Solution (Russ.), pp. 66–71, Moscow: Nauka.Google Scholar
Saucier, J. M. & Wang, J. C. (1972). Angular alteration of the DNA helix by E. coli RNA polymerase. Nature, New Biol. 239, 167–170.Google ScholarPubMed
Schofield, P., Hoffman, B. M. & Rich, A. (1970). Spin-labelling studies of aminoacyl transfer ribonucleic acid. Biochemistry, N.Y. 9, 2525–2533.CrossRefGoogle Scholar
Schwarz, U., Lührmann, R. & Gassen, H. G. (1974) On the mRNA induced conformational change of AA-tRNA exposing the TCG sequence for binding to the SOS ribosomal subunit. Biochem. biophys. Res. Commun. 56, 807–811.CrossRefGoogle Scholar
Siebenlist, U. (1979). RNA polymerase unwinds on II-base pair segment of a phage T7 promoter. Nature, Lond. 279, 651–652.CrossRefGoogle Scholar
Simonov, V. V., Rjabchenko, N. I. & Poverenny, A. M. (1967). Study on DNA melting of differential spectroscopy in the presence of formaldehyde. Mol. Biol. (USSR) 1, 297–301.Google Scholar
Smith, I. C. P. & Yamane, T. (1967). Spin-labelled nucleic acids. Proc. natn. Acad. Sci. U.S.A. 58, 884–887.CrossRefGoogle Scholar
Sprinzl, M., Krämer, E. & Stehlik, D. (1974). On the structure of phenylalanine tDNA from yeast. Eur. J. Biochem. 49, 595–605.CrossRefGoogle Scholar
Sukhorukov, B. I. (1973). Spin label studies of DNA. Stud. Biophys. B40, 33–40.Google Scholar
Suddath, F. L., Quigley, G. J., McPherson, A., Sneden, D., Kim, J. J., Kim, S. H. & Rich, A. (1974). Three-dimensional structure of yeast tRNAPhe at 30 Å resolution. Nature, Lond. 248, 20–24.CrossRefGoogle Scholar
Surzycki, S., Surzycki, J., Delorbe, W. & Gussin, G. (1976). In vitro transcription of adenovirus 2 DNA. II. Quantification and localization of promoters for E. coli RNA polymerase. Molec. Gen. Genet. 143, 177–185.CrossRefGoogle ScholarPubMed
Thomas, G. J., Chen, M. C., Lord, R. C., Kotsiopoulos, P. S., Tritton, T. R. & Mohr, S. C. (1973). Transfer RNA: change of conformation upon aminoacylation determined by Raman spectroscopy. Biochem. biophys. Res. Commun. 54, 570–577.CrossRefGoogle ScholarPubMed
Travers, A. (1974 a). On the nature of DNA promoter conformations. Eur. J. Biochem. 47, 435–442.CrossRefGoogle ScholarPubMed
Travers, A. (1974 b). RNA polymerase-promoter interactions: some general principles. Cell 3, 97–103.CrossRefGoogle ScholarPubMed
Tunis-Schneider, M. J. B. & Maestre, M. F. (1970). Circular dichroism spectra of oriented and unoriented deoxyribonucleic acid films – A preliminary study. J. molec. Biol. 52, 521–541.CrossRefGoogle ScholarPubMed
Vogel, S. V., Slepneva, I. A. & Backer, J. M. (1975). Influence of Mn2+ ion coordination on tRNAva1, macrostructure and determination of some coordination sites of Mn2+ ions in tRNAVa11. Biopolymers 14, 2445–2456.Google Scholar
Wang, J. C. (1974). Interaction between twisted DNAs and enzymes: the effects of superhelical turns. J. molec. Biol. 87, 797–813.CrossRefGoogle ScholarPubMed
Wartell, R. M. & Burd, J. F. (1976). Evidence for long range interactions in DNA: analysis of melting curves of block polymers d(C15A15). d(T15- G15), d(C20A15). d(T15G20) and d(C20A10). d(T10G20). Biopolymers 15, 1461–1479.CrossRefGoogle Scholar
Wasserman, A. M., Kuznetsov, A. G., Kovarskii, A. Z. & Buchachenko, A. Z. (1971). Anisotropic rotational diffusion of stable nitroxide radicals. Zh. Strukt. Khimiya (USSR), 12, 609–619.Google Scholar
Watanabe, K. & Imahori, K. (1971). The conformational difference between tRNAfMet and formylmethionyl-tRNAfMet from E. coli. Biochem. biophys. Res. Commun. 45, 488–494.CrossRefGoogle Scholar
Wells, R. D., Blakesley, R. W., Burd, J. F., Chan, H. W., Dodgson, J. B., Hardies, S. C., Horn, G. T., Jensen, K. F., Larson, J. E., Nes, J. F., Slesing, E. & Wartell, R. W. (1977). The role of DNA structure in genetic regulation. CRS crit. Rev. Biochem. 4, 305–340.Google Scholar
Wong, Y. P., Reid, B. R. & Kearns, D. R. (1973). Conformation of charged and uncharged phenylalanine tRNA. Proc. natn. Acad. Sci. U.S.A., 70, no. 8, pp. 2193–2195.CrossRefGoogle Scholar
Wong, K. L., Wong, Y. P. & Kearns, D. R. (1975). Investigation of the thermal unfolding of secondary and tertiary structure in E. coli tRNAfMct by high-resolution NMR. Biopolymers 14, 749–762.CrossRefGoogle Scholar
Zavriev, S. K., Grigoryan, L. G., Krilova, I. & Karmanov, P. A. (1973). A spin-label for RNA study. Biochem. biophys. Res., Commun. 54, 123–126.CrossRefGoogle ScholarPubMed
Zavriev, S. K., Grigoryan, G. L., Minchenkova, L. E. & Ivanov, V. I. (1974 a). The study of conformational transitions within two-stranded state of spin-labelled DNA. Molecular Biology (USSR) 8, 775–783.Google Scholar
Zavriev, S. K., Grigoryan, G. L., Karmanov, P. A., Rtvealishvili, G. A. & Rozantsev, E. G. (1974 b). Study of ribosomal RNA and synthetic polyribonucleotides by the spin label method. Molekular Biology (USSR) 8, 302–309.Google ScholarPubMed
Zhiltsova, V. A., Ulanov, B. P., Kruglyakova, K. E. & Gindin, L. G. (1967). The influence of ionic strength and some other factors upon the kinetic of DNA denaturation under UV-irradiation. Biofizika (USSR) 12, 535–537.Google Scholar