Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-25T19:32:10.828Z Has data issue: false hasContentIssue false
  • English
  • Français

Improved Collagen Extraction by Modified Longin Method

Published online by Cambridge University Press:  18 July 2016

T A Brown ,
D E Nelson ,
J S Vogel  and
J R Southon
T A Brown
Affiliation:
Department of Archeology, Simon Fraser University Burnaby, BC, V5A 1S6 Canada
D E Nelson
Affiliation:
Department of Archeology, Simon Fraser University Burnaby, BC, V5A 1S6 Canada
J S Vogel*
Affiliation:
Department of Archeology, Simon Fraser University Burnaby, BC, V5A 1S6 Canada
J R Southon*
Affiliation:
Department of Archeology, Simon Fraser University Burnaby, BC, V5A 1S6 Canada
*
∗∗ Also at: Tandem Accelerator Laboratory, McMaster University, Hamilton, Ontario L8S 4K1, Canada
∗∗ Also at: Tandem Accelerator Laboratory, McMaster University, Hamilton, Ontario L8S 4K1, Canada
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

A re-evaluation of the Longin collagen-extraction method shows that a lower reflux temperature reduces degradation of protein (“collagen”) remnants. This allows additional purification through ultrafiltration to isolate the >30kDalton fraction of the reflux product.

Type
Research Article
Information
Radiocarbon , Volume 30 , Issue 2 , 1988 , pp. 171 - 177
Copyright
Copyright © The American Journal of Science 

References

Doberenz, A R and Wyckoff, R W, 1967, Fine structure of fossil collagen: Nat Acad Sci Proc, v 57, p 539–541.Google Scholar
Gurfinkel, D M, 1987, Comparative study of the radiocarbon dating of different bone collagen preparations: Radiocarbon, v 29, no. 1, p 45–52.CrossRefGoogle Scholar
Laemmli, U K 1970, Cleavage of structural proteins during the assembly of the head of Bacteriophage T4: Nature, v 227, p 680–685.CrossRefGoogle ScholarPubMed
Longin, R, 1971, New method of collagen extraction for radiocarbon dating: Nature,, v 230, Mellars, P A, Bricker, H M, Gowlett, J A J and Hedges, R E M, 1987, Radiocarbon accelerator dating of French Upper Palaeolithic sites: Current Anthropology, v 28, no. 1, p 128–133.Google Scholar
Nelson, D E, Vogel, J S, Southon, J R and Brown, T A, 1986, Accelerator radiocarbon dating at SFU, in Stuiver, M and Kra, R S, eds, Internatl 14C conf, 12th, Proc: Radiocarbon, v 28, no. 2A, p 215–222.Google Scholar
Piez, K A, 1984, Molecular and aggregate structures of the collagens, in Piez, K A and Reddi, A H, eds, Extracellular matrix biochemistry: New York, Elsevier p 1–39 Google Scholar
Piez, K A and Reddi, A H, eds, 1984, Extracellular matrix biochemistry: New York Elsevier, 473 p.Google Scholar
Stafford, T W Jr, Jull, A J T, Brendel, K, Duhamel, R C and Donahue, D, 1987, Study of bone radiocarbon dating accuracy at the University of Arizona NSF Accelerator Facility for Radioisotope Analysis: Radiocarbon, v 29, no. 1, p 24–44.CrossRefGoogle Scholar
Stevenson, F J, 1982, Humus chemistry; genesis, composition, reactions: New York, Wiley-Interscience, 443 p.Google Scholar
Tuross, N, Eyre, D R, Holtrop, M E, Glimcher, M J and Hare, P E, 1980, Collagen in fossil bones, in Hare, P E Hoering, T C and King, K Jr, eds, Biogeochemistry of amino acids: New York, John Wiley & Sons, p 53–63.Google Scholar