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Preparative High-Performance Liquid Chromatographic Separation of Individual Amino Acids Derived from Fossil Bone Collagen

Published online by Cambridge University Press:  18 July 2016

Gert Jaap van Klinken  and
Willem G Mook
Gert Jaap van Klinken
Affiliation:
Centrum voor Isotopenonderzoek, State University of Groningen, Westersingel 34, 9718 CM Groningen, The Netherlands
Willem G Mook
Affiliation:
Centrum voor Isotopenonderzoek, State University of Groningen, Westersingel 34, 9718 CM Groningen, The Netherlands
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Abstract

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Extracting a series of amino acids from fossil “collagen” makes it possible to compare chemically well-characterized molecules from one bone sample, both chemically and by isotopic analysis. Thus, the integrity of collagen samples and possible contamination can be checked. We describe the routine high-performance liquid chromatographic extraction of five amino acids: glycine, hydroxyproline, glutamate, threonine and alanine. Humates are eliminated during the extraction, which can be monitored continuously.

Type
Articles
Information
Radiocarbon , Volume 32 , Issue 2 , 1990 , pp. 155 - 164
Copyright
Copyright © The American Journal of Science 

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