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A general role for splicing enhancers in exon definition

Published online by Cambridge University Press:  03 October 2002

BIANCA J. LAM  and
KLEMENS J. HERTEL
BIANCA J. LAM
Affiliation:
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine, California 92697-4025, USA
KLEMENS J. HERTEL
Affiliation:
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine, California 92697-4025, USA
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Abstract

Exonic splicing enhancers (ESEs) facilitate exon definition by assisting in the recruitment of splicing factors to the adjacent intron. Here we demonstrate that suboptimal 5′ and 3′ splice sites are activated independently by ESEs when they are located on different exons. However, when they are situated within a single exon, the same weak 5′ and 3′ splice sites are activated simultaneously by a single ESE. These findings demonstrate that a single ESE promotes the recognition of both exon/intron junctions within the same step during exon definition. Our results suggest that ESEs recruit a multicomponent complex that minimally contains components of the splicing machinery required for 5′ and 3′ splice site selection.

Keywords

spliceosomesplice site recognitionsplicing regulationU1 snRNPU2AF
Type
Research Article
Information
RNA , Volume 8 , Issue 10 , October 2002 , pp. 1233 - 1241
Copyright
2002 RNA Society

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