Hostname: page-component-7dd5485656-dk7s8 Total loading time: 0 Render date: 2025-10-31T16:12:57.454Z Has data issue: false hasContentIssue false
  • English
  • Français

Suppression of eukaryotic translation termination by selected RNAs

Published online by Cambridge University Press:  08 December 2000

JASON CARNES ,
LUDMILA FROLOVA ,
SHAWN ZINNEN ,
GABRIÈLE DRUGEON ,
MICHEL PHILLIPPE ,
JUST JUSTESEN ,
ANNE-LISE HAENNI ,
LESLIE LEINWAND ,
LEV L. KISSELEV  and
MICHAEL YARUS
JASON CARNES
Affiliation:
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
LUDMILA FROLOVA
Affiliation:
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 117984 Moscow, Russia
SHAWN ZINNEN
Affiliation:
Ribozyme Pharmaceuticals, Inc., Boulder, Colorado 80301-5411, USA
GABRIÈLE DRUGEON
Affiliation:
Institut Jacques Monod, 75251 Paris Cedex 05, France
MICHEL PHILLIPPE
Affiliation:
Département de Biologie Génétique du Développement, Centre National de la Recherche Scientifique Unité Propre de Recherche 41, 35043 Rennes Cedex, France
JUST JUSTESEN
Affiliation:
Department of Molecular and Structural Biology, University of Aarhus, DK-8000 Aarhus C, Denmark
ANNE-LISE HAENNI
Affiliation:
Institut Jacques Monod, 75251 Paris Cedex 05, France
LESLIE LEINWAND
Affiliation:
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
LEV L. KISSELEV
Affiliation:
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 117984 Moscow, Russia
MICHAEL YARUS
Affiliation:
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80309-0347, USA
Get access

Abstract

Using selection-amplification, we have isolated RNAs with affinity for translation termination factors eRF1 and eRF1[bull]eRF3 complex. Individual RNAs not only bind, but inhibit eRF1-mediated release of a model nascent chain from eukaryotic ribosomes. There is also significant but weaker inhibition of eRF1-stimulated eRF3 GTPase and eRF3 stimulation of eRF1 release activity. These latter selected RNAs therefore hinder eRF1[bull]eRF3 interactions. Finally, four RNA inhibitors of release suppress a UAG stop codon in mammalian extracts dependent for termination on eRF1 from several metazoan species. These RNAs are therefore new specific inhibitors for the analysis of eukaryotic termination, and potentially a new class of omnipotent termination suppressors with possible therapeutic significance.

Keywords

aptamerhumaninhibitorrelease factorstop codon

Information

Type
Research Article
Information
RNA , Volume 6 , Issue 10 , October 2000 , pp. 1468 - 1479
Copyright
2000 RNA Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable