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Cloning and characterization of a COBRA-like gene expressed de novo during maize germination

Published online by Cambridge University Press:  22 February 2007

Felipe Cruz-García ,
Alberto Gómez ,
José Juan Zúñiga ,
Javier Plasencia  and
Jorge M. Vázquez-Ramos
Felipe Cruz-García
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México DF, 04510, México
Alberto Gómez
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México DF, 04510, México
José Juan Zúñiga
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México DF, 04510, México
Javier Plasencia
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México DF, 04510, México
Jorge M. Vázquez-Ramos*
Affiliation:
Departamento de Bioquímica, Facultad de Química, UNAM. Avenida Universidad y Copilco, México DF, 04510, México
*
*Correspondence Fax: +52 5622 5284, Email: jorman@servidor.unam.mx
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Abstract

The search for germination-specific genes has been a laborious and unrewarding task, since many of the genes expressed during germination are also expressed in embryogenesis or in other developmental stages. By using mRNA differential display of transcript populations from maize (Zea mays L.) embryo axes, germinated for different times with or without a previous osmopriming treatment, a 682 bp cDNA was isolated that was present only after 24 h germination, and absent during osmopriming or during early germination. Screening of a cDNA library using the 682 bp probe yielded a 1554 bp cDNA that contained an open reading frame coding for 436 amino acids. This gene, referred to as ZmAA9-24, was expressed in root tissues, but was not detected in shoot or leaf tissues. Expression of ZmAA9-24 occurred earlier during germination (by 15 h) if embryo axes were imbibed in the presence of cytokinins or if seeds were previously osmoprimed. The predicted protein sequence of ZmAA9-24 is 39.6% identical to the product of the recently identified Arabidopsis gene COBRA (54.5% in the central region), which appears to participate in the regulation of cell expansion, particularly in roots, and belongs to the glycosylphosphatidylinositol (GPI)-anchored protein family. ZmAA9-24 expression might be regulated by both cell expansion and the cell cycle, processes that have a central role during seed germination.

Keywords

ZmAA9-24 geneCOBRA genecell elongationZea mays L.primingbenzyladenine
Type
Research Article
Information
Seed Science Research , Volume 13 , Issue 3 , September 2003 , pp. 209 - 217
Copyright
Copyright © Cambridge University Press 2003

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