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Development and potential of genetically engineered oilseeds

Published online by Cambridge University Press:  22 February 2007

John M. Dyer  and
Robert T. Mullen
John M. Dyer*
Affiliation:
USDA-ARS Southern Regional Research Center, 1100 Robert E. Lee Blvd, New Orleans, LA 70124, USA
Robert T. Mullen
Affiliation:
Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
*
Correspondence: Fax: +1 504 286 4419 Email: jdyer@srrc.ars.usda.gov
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Abstract

Oilseed crops are major sources of oils for human nutrition, and an increasing proportion is also being utilized for industrial purposes. Recent advances in our understanding of the basic biochemistry of seed oil biosynthesis, coupled with identification of genes for oilseed modification, have set the stage for the genetic engineering of oilseed crops that produce ‘designer’ plant seed oils tailored for specific applications. In this review we provide an overview of seed oil biosynthesis and highlight the enzymatic steps that have already been targeted for genetic manipulation, with the end goal of producing seed oils containing desired amounts of fatty acid components. Furthermore, we describe the identification of genes from various wild plant species that are capable of producing structurally diverse fatty acids, and how these advances open the door to the production of entirely novel oils in conventional oilseed crops. Transgenic oilseeds producing high amounts of these novel fatty acids represent renewable sources of raw materials that may compete with, and eventually replace, some petrochemicals that are derived from non-renewable crude oil.

Keywords

fatty acidgenetic engineeringoil biosynthesisoilseedwax
Type
Invited Review
Information
Seed Science Research , Volume 15 , Issue 4 , December 2005 , pp. 255 - 267
Copyright
Copyright © Cambridge University Press 2005

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References

Abbadi, A., Domergue, F., Bauer, J., Napier, J.A., Welti, R., Zahringer, U., Cirpus, P. and Heinz, E. (2004) Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation. Plant Cell 16, 2734–2748.Google Scholar
Auld, D.L., Rolfe, R.D. and McKeon, T.A. (2001) Development of castor with reduced toxicity. Journal of New Seeds 3, 61–69.CrossRefGoogle Scholar
Bao, X. and Ohlrogge, J. (1999) Supply of fatty acid is one limiting factor in the accumulation of triacylglycerol in developing embryos. Plant Physiology 120, 1057–1062.Google Scholar
Bockisch, M. (1998) Fats and oils handbook. Champaign, IL, IAOCS Press.Google Scholar
Bouvier-Nave, P., Benveniste, P., Oelkers, P., Sturley, S.L. and Schaller, H. (2000) Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase. European Journal of Biochemistry 267, 85–96.CrossRefGoogle ScholarPubMed
Broun, P. and Somerville, C. (1997) Accumulation of ricinoleic, lesquerolic, and densipolic acids in seeds of transgenic Arabidopsis plants that express a fatty acyl hydroxylase cDNA from castor bean. Plant Physiology 113, 933–942.Google Scholar
Broun, P., Boddupalli, S. and Somerville, C. (1998) A bifunctional oleate 12-hydroxylase:desaturase from Lesquerella fendleri. Plant Journal 13, 201–210.Google Scholar
Broun, P., Gettner, S. and Somerville, C. (1999) Genetic engineering of plant lipids. Annual Review of Nutrition 19, 197–216.CrossRefGoogle ScholarPubMed
Buchanan, B.B., Gruissen, W. and Jones, R.L. (2000) Biochemistry and molecular biology of plants. Rockville, Maryland, American Society of Plant Physiologists.Google Scholar
Burg, D.A. and Kleiman, R. (1991) Preparation of meadowfoam dimer acids and dimer esters, and their use as lubricants. Journal of the American Oil Chemists Society 68, 600–603.CrossRefGoogle Scholar
Cahoon, E.B., Shanklin, J. and Ohlrogge, J.B. (1992) Expression of a coriander desaturase results in petroselinic acid production in transgenic tobacco. Proceedings of the National Academy of Sciences, USA 89, 11184–11188.Google Scholar
Cahoon, E.B., Cranmer, A.M., Shanklin, J. and Ohlrogge, J.B. (1994) Δ 6 Hexadecenoic acid is synthesized by the activity of a soluble Δ 6 palmitoyl-acyl carrier protein desaturase in Thunbergia alata endosperm. Journal of Biological Chemistry 269, 27519–27526.Google Scholar
Cahoon, E.B., Coughlan, S.J. and Shanklin, J. (1997) Characterization of a structurally and functionally diverged acyl-acyl carrier protein desaturase from milkweed seed. Plant Molecular Biology 33, 1105–1110.Google Scholar
Cahoon, E.B., Shah, S., Shanklin, J. and Browse, J. (1998) A determinant of substrate specificity predicted from the acyl-acyl carrier protein desaturase of developing cat's claw seed. Plant Physiology 117, 593–598.CrossRefGoogle ScholarPubMed
Cahoon, E.B., Carlson, T.J., Ripp, K.G., Schweiger, B.J., Cook, G.A., Hall, S.E. and Kinney, A.J. (1999) Biosynthetic origin of conjugated double bonds: Production of fatty acid components of high-value drying oils in transgenic soybean embryos. Proceedings of the National Academy of Sciences, USA 96, 12935–12940.Google Scholar
Cahoon, E.B., Marillia, E.F., Stecca, K.L., Hall, S.E., Taylor, D.C. and Kinney, A.J. (2000) Production of fatty acid components of meadowfoam oil in somatic soybean embryos. Plant Physiology 124, 243–251.CrossRefGoogle ScholarPubMed
Cahoon, E.B., Ripp, K.G., Hall, S.E. and Kinney, A.J. (2001) Formation of conjugated Δ 8, Δ 10 double bonds by Δ 12 -oleic acid desaturase related enzymes. Biosynthetic origin of calendic acid. Journal of Biological Chemistry 276, 2637–2643.CrossRefGoogle ScholarPubMed
Cahoon, E.B., Ripp, K.G., Hall, S.E. and McGonigle, B. (2002) Transgenic production of epoxy fatty acids by expression of a cytochrome P450 enzyme from Euphorbia lagascae seed. Plant Physiology 128, 615–624.CrossRefGoogle ScholarPubMed
Davies, H.M. (1993) Medium chain acyl-ACP hydrolysis activities of developing oilseeds. Phytochemistry 33, 1353–1356.Google Scholar
Davies, H.M., Anderson, L., Fan, C. and Hawkins, D.J. (1991) Developmental induction, purification, and further characterization of 12:0-ACP thioesterase from immature cotyledons of Umbellularia californica. Archives of Biochemistry and Biophysics 290, 37–45.Google Scholar
Dehesh, K., Jones, A., Knutzon, D.S. and Voelker, T.A. (1996) Production of high levels of 8:0 and 10:0 fatty acids in transgenic canola by overexpression of Ch FatB2, a thioesterase cDNA from Cuphea hookeriana. Plant Journal 9, 167–172.Google Scholar
Domergue, F., Abbadi, A. and Heinz, E. (2005) Relief for fish stocks: oceanic fatty acids in transgenic oilseeds. Trends in Plant Science 10, 112–116.Google Scholar
Dyer, J.M., Chapital, D.C., Kuan, J.C., Mullen, R.T., Turner, C., McKeon, T.A. and Pepperman, A.B. (2002) Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung. Implications for the evolution of plant fatty acid diversity. Plant Physiology 130, 2027–2038.CrossRefGoogle ScholarPubMed
Eccleston, V.S. and Ohlrogge, J.B. (1998) Expression of lauroyl-acyl carrier protein thioesterase in Brassica napus seeds induces pathways for both fatty acid oxidation and biosynthesis and implies a set point for triacylglycerol accumulation. Plant Cell 10, 613–621.Google Scholar
Edem, D.O. (2002) Palm oil: biochemical, physiological, nutritional, hematological, and toxicological aspects: A review. Plant Foods for Human Nutrition 57, 319–341.CrossRefGoogle ScholarPubMed
Erhan, S.M., Kleiman, R. and Isbell, T.A. (1993) Estolides from meadowfoam oil fatty acids and other monounsaturated fatty acids. Journal of the American Oil Chemists Society 70, 461–465.Google Scholar
Facciotti, M.T., Bertain, P.B. and Yuan, L. (1999) Improved stearate phenotype in transgenic canola expressing a modified acyl-acyl carrier protein thioesterase. Nature Biotechnology 17, 593–597.Google Scholar
Fernandez-Cornejo, J. and McBride, W.D. (2002) Genetically engineered crops: U.S. adoption and impacts. Agricultural Outlook 294, 24–27.Google Scholar
Fitch-Haumann, B. (1997) Structured lipids allow fat tailoring. Inform 8, 1004–1011.Google Scholar
Graef, G.L., Miller, L.A., Fehr, W.R. and Hammon, E.G. (1985) Fatty acid development in a soybean mutant with high stearic acid. Journal of the American Oil Chemists Society 62, 773–775.Google Scholar
Graham, S.A. (1989) Cuphea: A new plant source of medium-chain fatty acids. Critical Reviews in Food Science and Nutrition 28, 139–173.Google Scholar
Graham, S.A., Hirsinger, F. and Robbelen, G. (1981) Fatty acids of Cuphea ( Lythraceae ) seed lipids and their systematic significance. American Journal of Botany 68, 908–917.CrossRefGoogle Scholar
Grayburn, W.S. and Hildebrand, D.F. (1995) Progeny analysis of tobacco that express a mammalian Δ 9 desaturase. Journal of the American Oil Chemists Society 72, 317–321.CrossRefGoogle Scholar
Gunstone, F.D. (1998) Movements towards tailor-made fats. Progress in Lipid Research 37, 277–305.CrossRefGoogle ScholarPubMed
Hawkins, D.J. and Kridl, J.C. (1998) Characterization of acyl-ACP thioesterases of mangosteen ( Garcinia mangostana ) seed and high levels of stearate production in transgenic canola. Plant Journal 13, 743–752.Google Scholar
Herbers, K. and Sonnewald, U. (1999) Production of new/modified proteins in transgenic plants. Current Opinion in Biotechnology 10, 163–168.Google Scholar
Heyer, A.G., Lloyd, J.R. and Kossmann, J. (1999) Production of modified polymeric carbohydrates. Current Opinion in Biotechnology 10, 169–174.CrossRefGoogle ScholarPubMed
Huang, A.H.C. (1992) Oil bodies and oleosins in seeds. Annual Review of Plant Physiology and Plant Molecular Biology 43, 177–200.CrossRefGoogle Scholar
Huang, A.H.C. (1996) Oleosins and oil bodies in seeds and other organs. Plant Physiology 110, 1055–1061.Google Scholar
Huang, J.K., Rozelle, S., Pray, C. and Wang, Q.F. (2002) Plant biotechnology in China. Science 295, 674–677.CrossRefGoogle ScholarPubMed
James, C. (2001) Global status of commercialized transgenic crops Ithaca, New York, International Service for the Acquisition of Agri-Biotech Applications.Google Scholar
Kinney, A.J. (1996a) Improving soybean seed quality. Nature Biotechnology 14, 946CrossRefGoogle Scholar
Kinney, A.J. (1996b) β-Ketoacyl-ACP synthetase II genes from plants. US Patent 5,500,361Google Scholar
Kinney, A.J., Knowlton, S., Cahoon, E.B. and Hitz, W.D. (1998) Re-engineering oilseed crops to produce industrially useful fatty acids. pp. 623–628. in Sánchez, J.;, Cerdá-Olmedo, E.;, Martinez-Force, E. (Eds) Advances in plant lipid research, Seville, Spain, University of Seville Press.Google Scholar
Knutzon, D.S., Thompson, G.A., Radke, S.E., Johnson, W.B., Knauf, V.C. and Kridl, J.C. (1992) Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carrier protein desaturase gene. Proceedings of the National Academy of Sciences, USA 89, 2624–2628.CrossRefGoogle ScholarPubMed
Knutzon, D.S., Hayes, T.R., Wyrick, A., Xiong, H., Davies, H.M. and Voelker, T.A. (1999) Lysophosphatidic acid acyltransferase from coconut endosperm mediates the insertion of laurate at the sn-2 position of triacylglycerols in lauric rapeseed oil and can increase total laurate levels. Plant Physiology 120, 739–746.CrossRefGoogle ScholarPubMed
Lardizabal, K.D., Metz, J.G., Sakamoto, T., Hutton, W.C., Pollard, M.R. and Lassner, M.W. (2000) Purification of a jojoba embryo wax synthase, cloning of its cDNA, and production of high levels of wax in seeds of transgenic Arabidopsis. Plant Physiology 122, 645–655.Google Scholar
Lavers, B. (2002) Canola in Canada. Oils and Fats International 18, 18–19.Google Scholar
Lee, M., Lenman, M., Banas, A., Bafor, M., Singh, S., Schweizer, M., Nilsson, R., Liljenberg, C., Dahlqvist, A., Gummeson, P.O., Sjodahl, S., Green, A. and Stymne, S. (1998) Identification of non-heme diiron proteins that catalyze triple bond and epoxy group formation. Science 280, 915–918.Google Scholar
Liu, Q., Singh, S.P. and Green, A.G. (2002) High-stearic and high-oleic cottonseed oils produced by hairpin RNA-mediated post-transcriptional gene silencing. Plant Physiology 129, 1732–1743.CrossRefGoogle ScholarPubMed
McKeon, T.A., Lin, J.T. and Stafford, A.E. (1999) Biosynthesis of ricinoleate in castor oil. Advances in Experimental Medicine and Biology 464, 37–47.Google Scholar
Miquel, M.F. and Browse, J.A. (1994) High-oleate oilseeds fail to develop at low temperature. Plant Physiology 106, 421–427.CrossRefGoogle ScholarPubMed
Moon, H., Hazebroek, J. and Hildebrand, D.F. (2000) Changes in fatty acid composition in plant tissues expressing a mammalian Δ 9 desaturase. Lipids 35, 471–479.CrossRefGoogle Scholar
Murphy, D.J. (1993) Structure, function, and biogenesis of storage lipid bodies and oleosins in plants. Progress in Lipid Research 32, 247–280.Google Scholar
Murphy, D.J. (1999) Production of novel oils in plants. Current Opinion in Biotechnology 10, 175–180.Google Scholar
Ohlrogge, J. and Browse, J. (1995) Lipid biosynthesis. Plant Cell 7, 957–970.Google ScholarPubMed
Osorio, J., Fernandez-Martinez, J., Mancha, M. and Garces, R. (1995) Mutant sunflowers with high concentration of saturated fatty acids in their oil. Crop Science 35, 739–742.Google Scholar
Padley, F.B., Gunstone, F.D. and Harwood, J.L. (1994) Occurrence and characteristics of oil and fats. pp. 49–170. Gunstone, F.D.;, Harwood, J.L.;, Padley, F.B. (Eds) The lipid handbook. London, Chapman & Hall.Google Scholar
Parmenter, D.L., Boothe, J.G., van Rooijen, G.J.H., Yeung, E.C. and Moloney, M.M. (1995) Production of biologically active hirudin in plant seeds using oleosin partitioning. Plant Molecular Biology 29, 1167–1180.Google Scholar
Phillips, B.E., Smith, C.R. and Tallent, W.H. (1971) Glycerides of Limnanthes douglasii seed oil. Lipids 6, 93–99.Google Scholar
Polashock, J.J., Chin, C.K. and Martin, C.E. (1992) Expression of the yeast Δ9 fatty acid desaturase in Nicotiana tobaccum. Plant Physiology 100, 894–901.CrossRefGoogle Scholar
Pollard, M.R. and Stumpf, P.K. (1980) Biosynthesis of C 20 and C 22 fatty acids by developing seeds of Limnanthes alba: chain elongation and Δ 5 desaturation. Plant Physiology 66, 649–655.Google Scholar
Pollard, M.R., Anderson, L., Fan, C., Hawkins, D.J. and Davies, H.M. (1991) A specific acyl-ACP thioesterase implicated in medium-chain fatty acid production in immature cotyledons of Umbellularia californica. Archives of Biochemistry and Biophysics 284, 306–312.CrossRefGoogle ScholarPubMed
Qiu, X., Reed, D.W., Hong, H.P., MacKenzie, S.L. and Covello, P.S. (2001) Identification and analysis of a gene from Calendula officinalis encoding a fatty acid conjugase. Plant Physiology 125, 847–855.Google Scholar
Richter, K.D., Mukherjee, K.D. and Weber, N. (1996) Fat infiltration in liver of rats induced by different dietary plant oils: high oleic-, medium oleic- and high petroselinic acid-oils. Zeitschrift Fur Ernahrungwissenschaft 35, 241–248.Google Scholar
Roesler, K., Shintani, D., Savage, L., Boddupalli, S. and Ohlrogge, J. (1997) Targeting of the Arabidopsis homomeric acetyl-coenzyme A carboxylase to plastids of rapeseeds. Plant Physiology 113, 75–81.Google Scholar
Roughan, P.G. (1997) Stromal concentrations of coenzyme A and its esters are insufficient to account for rates of chloroplast fatty acid synthesis: evidence for substrate channelling within the chloroplast fatty acid synthase. Biochemical Journal 327, 267–273.Google Scholar
Sayanova, O.V. and Napier, J.A. (2004) Eicosapentaenoic acid: biosynthetic routes and the potential for synthesis in transgenic plants. Phytochemistry 65, 147–158.Google Scholar
Sayanova, O., Smith, M.A., Lapinskas, P., Stobart, A.K., Dobson, G., Christie, W.W., Shewry, P.R. and Napier, J.A. (1997) Expression of a borage desaturase cDNA containing an N-terminal cytochrome b 5 domain results in the accumulation of high levels of Δ 6 -desaturated fatty acids in transgenic tobacco. Proceedings of the National Academy of Sciences, USA 94, 4211–4216.Google Scholar
Schnurr, J.A., Shockey, J.M., de Boer, G.J. and Browse, J.A. (2002) Fatty acid export from the chloroplast. Molecular characterization of a major plastidial acyl-coenzyme A synthetase from Arabidopsis. Plant Physiology 129, 1700–1709.Google Scholar
Schultz, D.J., Cahoon, E.B., Shanklin, J., Craig, R., Cox-Foster, D.L., Mumma, R.O. and Medford, J.I. (1996) Expression of a Δ 9 14:0-acyl carrier protein fatty acid desaturase gene is necessary for the production of ω 5 anacardic acids found in pest-resistant geranium (Pelargonium xhortorum). Proceedings of the National Academy of Sciences, USA 93, 8771–8775.CrossRefGoogle ScholarPubMed
Shanklin, J. and Cahoon, E.B. (1998) Desaturation and related modifications of fatty acids. Annual Review of Plant Physiology and Plant Molecular Biology 49, 611–641.CrossRefGoogle Scholar
Singh, S., Thomaeus, S., Lee, M., Stymne, S. and Green, A. (2001) Transgenic expression of a Δ 12 -epoxygenase gene in Arabidopsis seeds inhibits accumulation of linoleic acid. Planta 212, 872–879.Google Scholar
Singh, S.P., Zhou, X.R., Liu, Q., Stymne, S. and Green, A.G. (2005) Metabolic engineering of new fatty acids in plants. Current Opinion in Plant Biology 8, 197–203.Google Scholar
Smith, C.R.J. (1970) Occurrence of unusual fatty acids in plants. Progress in the Chemistry of Fats and Other Lipids 11, 139–177.Google Scholar
Sperling, P., Lee, M., Girke, T., Zähringer, U., Stymne, S. and Heinz, E. (2000) A bifunctional Δ 6 -fatty acyl acetylenase/desaturase from the moss Ceratodon purpureus. A new member of the cytochrome b 5 superfamily. European Journal of Biochemistry 267, 3801–3811.CrossRefGoogle ScholarPubMed
Stahl, U., Carlsson, A.S., Lenman, M., Dahlqvist, A., Huang, B., Banas, W., Banas, A. and Stymne, S. (2004) Cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from Arabidopsis. Plant Physiology 135, 1324–1335.Google Scholar
Stender, S. and Dyerberg, J. (2004) Influence of trans fatty acids on health. Annals of Nutrition and Metabolism 48, 61–66.CrossRefGoogle ScholarPubMed
Stoutjesdijk, P.A., Singh, S.P., Liu, Q., Hurlstone, C.J., Waterhouse, P.A. and Green, A.G. (2002) hpRNA-mediated targeting of the Arabidopsis FAD2 gene gives highly efficient and stable silencing. Plant Physiology 129, 1723–1731.Google Scholar
Suh, M.C., Schultz, D.J. and Ohlrogge, J.B. (2002) What limits production of unusual monoenoic fatty acids in transgenic plants?. Planta 215, 584–595.Google Scholar
Thelen, J.J. and Ohlrogge, J.B. (2002) Metabolic engineering of fatty acid biosynthesis in plants. Metabolic Engineering 4, 12–21.Google Scholar
van de, Loo F.J., Broun, P., Turner, S. and Somerville, C. (1995) An oleate 12-hydroxylase from Ricinus communis L. is a fatty acyl desaturase homolog. Proceedings of the National Academy of Sciences, USA 92, 6743–6747.Google Scholar
Voelker, T. and Kinney, A.J. (2001) Variations in the biosynthesis of seed-storage lipids. Annual Review of Plant Physiology and Plant Molecular Biology 52, 335–361.Google Scholar
Voelker, T.A., Worrell, A.C., Anderson, L., Bleibaum, J., Fan, C., Hawkins, D.J., Radke, S.E. and Davies, H.M. (1992) Fatty acid biosynthesis redirected to medium chains in transgenic oilseed plants. Science 257, 72–74.Google Scholar
Voelker, T.A., Hayes, T.R., Cranmer, A.M., Turner, J.C. and Davies, H.M. (1996) Genetic engineering of a quantitative trait: metabolic and genetic parameters influencing the accumulation of laurate in rapeseed. Plant Journal 9, 229–241.CrossRefGoogle Scholar
Weber, N., Richter, K.D., Schulte, E. and Mukherjee, K.D. (1995) Petroselinic acid from dietary triacylglycerols reduces the concentration of arachidonic acid in tissue lipids of rats. Journal of Nutrition 125, 1563–1568.Google ScholarPubMed
Zou, J., Katavic, V., Giblin, E.M., Barton, D.L., MacKenzie, S.L., Keller, W.A. Hu, X., Taylor D.C. (1997) Modification of seed oil content and acyl composition in the Brassicaceae by expression of a yeast sn-2 acyltransferase gene. Plant Cell 9, 909–923.CrossRefGoogle ScholarPubMed