Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-10T15:58:45.118Z Has data issue: false hasContentIssue false
  • English
  • Français

Heat shock and recovery in aged wheat aleurone layers

Published online by Cambridge University Press:  19 September 2008

M.A. Livesley  and
C. M. Bray
M.A. Livesley
Affiliation:
Department of Biochemistry and Molecular Biology, University of Manchester, Oxford Road, Manchester M13 9PT, UK
C. M. Bray*
Affiliation:
Department of Biochemistry and Molecular Biology, University of Manchester, Oxford Road, Manchester M13 9PT, UK
*
* Correspondence
Get access Rights & Permissions [Opens in a new window]

Abstract

Ageing of wheat seeds (Triticum aestivum cv. Galahad, 1986 harvest) during storage is accompanied by an increase in the proportion of abnormal seedlings produced when aged seed lots germinate. The response of aleurone layers from normally and abnormally germinated seeds to heat shock has been investigated. [14C]-amino acid incorporation into aleurone layers from 3-d normally and abnormally germinated seeds diminished after 4 h at 42°C but the aleurone layers were able to recover significantly during a 5-h period at 20°C following heat shock. [35S]-methionine pulse-labelling of aleurone proteins showed that α-amylase isoenzyme synthesis was abolished upon heat shock in aleurone layers for both normally and abnormally germinated seeds and that only aleurone layers from normally germinated seeds were capable of recommencing substantial α-amylase synthesis during the recovery period. Heat shock caused aleurone layers from abnormally germinated seeds to synthesize polypeptides not synthesized by aleurone layers from normally germinated seeds under the same conditions. The synthesis of certain polypeptides by aleurone layers from abnormally germinated seeds was enhanced at 20°C, 42°C and during recovery compared with normally germinated seeds. These results are discussed in terms of the control of gene expression during heat shock and recovery during seed ageing.

Keywords

seed ageingaleuronegerminationheat shockwheat
Type
Research Papers
Information
Seed Science Research , Volume 3 , Issue 3 , September 1993 , pp. 179 - 186
Copyright
Copyright © Cambridge University Press 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Abernethy, R.H., Thiel, D.S., Petersen, N.S. and Helm, K. (1989) Thermotolerance is developmentally dependent in germinating wheat seed. Plant Physiology 89, 569–576.CrossRefGoogle ScholarPubMed
Belanger, F.C., Brodl, M.R. and Ho, T.H.D. (1986) Heat shock causes destabilization of specific mRNAs and destruction of E.R. in barley aleurone cells. Proceedings of the National Academy of Sciences, USA 83, 1354–1358.CrossRefGoogle Scholar
Bonner, W.M. and Laskey, R.A. (1974) A film detection method for tritium labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46, 83–88.CrossRefGoogle ScholarPubMed
Brodl, M.R. (1989) Regulation of the synthesis of normal proteins during heat shock. Physiologia Plantarum 75, 439–443.CrossRefGoogle Scholar
Buzin, C.H. and Petersen, N.S. (1982) A comparison of the multiple Drosophila heat shock proteins in cell lines and larval salivary glands by 2-Dgel electrophoresis. Journal of Molecular Biology 158, 181–201.CrossRefGoogle ScholarPubMed
Cuming, A.C. and Lane, B.G. (1979) Protein synthesis in imbibing wheat embryos. European Journal of Biochemistry 99, 217–224.CrossRefGoogle ScholarPubMed
Dell'Aquila, A., Zocchi, G., Lanzani, G.A. and De Leo, P. (1976) Different forms of EF1 and viability in wheat embryos. Phytochemistry 15, 1607–1610.CrossRefGoogle Scholar
Helm, K.W., Petersen, N.S. and Abernethy, R.H. (1989) The heat shock response of germinating embryos of wheat. Plant Physiology 90, 598–605.CrossRefGoogle ScholarPubMed
Ho, T.H.D. and Sachs, M.M. (1989) Stress induced proteins: characterization and the regulation of their synthesis. pp 347–378in, Marcus, A. (Ed.) The biochemistry of plants 15. Molecular Biology. New York, Academic Press.Google Scholar
Ho, T.H.D., Nolan, R.C., Lin, L.S., Brodl, M.R. and Brown, P.H. (1987) Regulation of gene expression in barley aleurone layers. pp. 34–39in Fox, J.E. and Jacobs, M. (Eds) Molecular biology of plant growth control. Alan R. Liss Inc. New York.Google Scholar
Livesley, M.A. and Bray, C.M. (1991) The effects of ageing upon α-amylase production and protein synthesis by wheat aleurone layers. Annals of Botany 68, 69–73.CrossRefGoogle Scholar
Mans, R.J. and Novelli, G.C. (1961) Measurement of the incorporation of radioactive amino acids into protein by a filter paper disc method. Archives of Biochemistry and Biophysics 94, 48–53.CrossRefGoogle Scholar
Osborne, D.J. (1983) Biochemical control systems operating in the early hours of germination. Canadian Journal of Botany 61, 3568–3577.CrossRefGoogle Scholar
Smith, C.A.D. and Bray, C.M. (1984) Polyadenylated RNA levels and macromolecular synthesis during loss of seed vigour. Plant Science Letters 34, 335–343.CrossRefGoogle Scholar
Wu, C., Wilson, S., Walker, B., Dawid, I., Paisley, T., Zimarino, V. and Veda, H. (1987) Purification and properties of Drosophila heat shock activator protein. Science 238, 1247–1253.CrossRefGoogle ScholarPubMed