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The re-establishment of desiccation tolerance in germinated radicles of Medicago truncatula Gaertn. seeds

Published online by Cambridge University Press:  22 February 2007

Julia Buitink ,
Benoit Ly Vu ,
Pascale Satour  and
Olivier Leprince
Julia Buitink*
Affiliation:
UMR 1191 Molecular Seed Physiology, INRA/Institut National d'Horticulture/Université d'Angers, 16 bd Lavoisier, Angers, 49045, France
Benoit Ly Vu
Affiliation:
UMR 1191 Molecular Seed Physiology, INRA/Institut National d'Horticulture/Université d'Angers, 16 bd Lavoisier, Angers, 49045, France
Pascale Satour
Affiliation:
UMR 1191 Molecular Seed Physiology, INRA/Institut National d'Horticulture/Université d'Angers, 16 bd Lavoisier, Angers, 49045, France
Olivier Leprince
Affiliation:
UMR 1191 Molecular Seed Physiology, INRA/Institut National d'Horticulture/Université d'Angers, 16 bd Lavoisier, Angers, 49045, France
*
*Correspondence Fax: +33 241 739309 Email: julia.buitink@angers.inra.fr
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Abstract

Germinated seeds of Medicago truncatula Gaertn. with a protruded radicle length of 2.7 mm did not survive drying below 0.2 g H2O g–1 dw, as indicated by vital stain assays and the absence of growth resumption after rehydration. The re-establishment of desiccation tolerance was achieved using an osmotic treatment with polyethylene glycol (PEG), combined with a cold treatment. The ability to regain desiccation tolerance after germination was restricted to a period of growth characterized by radicle lengths between 1 and 3 mm. After PEG treatment of germinated seeds with 2.7 mm long radicles at –1.7 MPa at 10°C for 3 d and subsequent drying to 0.04 g H2O g–1 dw, 90% survived and developed into normal seedlings after rehydration. Desiccation tolerance could also be re-established in excised radicles, demonstrating that cotyledons were not essential for this process. Upon PEG incubation, sucrose accumulated rapidly prior to the re-establishment of desiccation tolerance in germinated radicles, regardless of the presence of cotyledons. Induction of MtDHN (a dehydrin) gene expression was correlated with the re-establishment of desiccation tolerance. Furthermore, the PEG-induced expression of MtDHN was repressed when fluridone was added to the PEG solution.

Keywords

Medicago truncatuladesiccation tolerancesucrosedehydrinsgerminationosmotic shockabscisic acid
Type
Research Article
Information
Seed Science Research , Volume 13 , Issue 4 , December 2003 , pp. 273 - 286
Copyright
Copyright © Cambridge University Press 2003

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